Facilitated endospore detection for <i>Bacillus</i> spp. through automated algorithm‐based image processing

Biermann, R.; Niemeyer, Laura; Rösner, Laura; Ude, Christian; Lindner, Patrick; Bice, Ismet; Beutel, Sascha

doi:10.1002/elsc.202100137

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…These shells are made of a proteinaceous coat, consisting of S-layer proteins, aminopeptidases and flagellin [23]. This coating allows the bacteria to survive harsh external conditions to germinate in the gut, making them suitable for use in animal feed as probiotics [24]. Once sporulated in the gastrointestinal tract, these probiotics can release a wide range of active substances including enzymes, nutrients and antimicrobial compounds [25].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Properties of Bacillus Probiotics as Animal Growth Promoters

et al. 2023

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Antibiotic growth promoters (AGPs) suppress the growth of infectious pathogens. These pathogens negatively impact agricultural production worldwide and often cause health problems if left untreated. Here, we evaluate six Bacillus strains (BPR-11, BPR-12, BPR-13, BPR-14, BPR-16 and BPR-17), which are known for their ability to survive harsh environmental conditions, as AGP replacements in animal feed. Four of these Bacillus strains (BPR-11, BPR-14, BPR-16 and BPR-17) showed antimicrobial activity against the pathogenic strains Clostridium perfringens, Escherichia coli and Staphylococcus aureus at 25 μg/mL, with BPR-16 and BPR-17 also able to inhibit Pseudomonas aeruginosa and Salmonella enterica at 100 μg/mL. Further chemical investigation of BPR-17 led to the identification of eight metabolites, namely C16, C15, C14 and C13 surfactin C (1–4), maculosin (5), maculosine 2 (6), genistein (7) and daidzein (8). Purified compounds (1–4) were able to inhibit all the tested pathogens with MIC values ranging from 6.25 to 50 μg/mL. Maculosin (5) and maculosine 2 (6) inhibited C. perfringens, E. coli and S. aureus with an MIC of 25 μg/mL while genistein (7) and daidzein (8) showed no activity. An animal trial involving feeding BPR-11, BPR-16 and BPR-17 to a laboratory poultry model led to an increase in animal growth, and a decrease in feed conversion ratio and mortality. The presence of surfactin C analogues (3–4) in the gut following feeding with probiotics was confirmed using an LC–MS analysis. The investigation of these Bacillus probiotics, their metabolites, their impacts on animal performance indicators and their presence in the gastrointestinal system illustrates that these probiotics are effective alternatives to AGPs.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Properties of Bacillus Probiotics as Animal Growth Promoters

et al. 2023

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“…Their idea was to use ML in order to have a more efficient sample throughput with high accuracy [57]. Biermann et al used an algorithm-based image processing for the at-line determination of endospores from microscopic pictures [79]. Here, too, the aim was to achieve a faster result using ML.…”

Section: Artificial Intelligence and Machine Learningmentioning

confidence: 99%

“…Biermann et al. used an algorithm‐based image processing for the at‐line determination of endospores from microscopic pictures [ 79 ]. Here, too, the aim was to achieve a faster result using ML.…”

Section: Digitalization Strategiesmentioning

confidence: 99%

Digitalization concepts in academic bioprocess development

Habich,

Beutel

2024

Engineering in Life Sciences

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Digitalization with integrated devices, digital and physical assistants, automation, and simulation is setting a new direction for laboratory work. Even with complex research workflows, high staff turnover, and a limited budget some laboratories have already shown that digitalization is indeed possible. However, academic bioprocess laboratories often struggle to follow the trend of digitalization. Due to their diverse research circumstances, high variety of team composition, goals, and limitations the concepts are substantially different. Here, we will provide an overview on different aspects of digitalization and describe how academic laboratories successfully digitalized their working environment. The key aspect is the collaboration and communication between IT‐experts and scientific staff. The developed digital infrastructure is only useful if it supports the laboratory worker and does not complicate their work. Thereby, laboratory researchers have to collaborate closely with IT‐experts in order for a well‐developed and maintainable digitalization concept that fits their individual needs and level of complexity. This review may serve as a starting point or a collection of ideas for the transformation toward a digitalized laboratory.

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“…After an eight step process the autolysis of the mother cell releases the matured endospore [1,2,[6][7][8][9]. This cell type is largely dehydrated [10] and includes a typical biomarker called dipicolinic acid [6,[11][12][13]. Especially the nutrient depletion acts as the main trigger for sporulation and, thus prevents active growth and sporulation at the same time [1,3].…”

Section: Endospores and Sporulation Of Bacillus Sppmentioning

confidence: 99%

Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium

Biermann,

Rösner,

Beyer

et al. 2023

Engineering in Life Sciences

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Bacillus coagulans is a promising probiotic, because it combines probiotic properties of Lactobacillus and the ability of Bacillus to form endospores. Due to this hybrid relationship, cultivation of this organism is challenging. As the probiotics market continues to grow, there is a new focus on the production of these microorganisms. In this work, a strain‐specific bioprocess for B. coagulans was developed to support growth on one hand and ensure sporulation on the other hand. This circumstance is not trivial, since these two metabolic states are contrary. The developed bioprocess uses a modified chemically defined medium which was further investigated in a one‐factor‐at‐a‐time assay after adaptation. A transfer from the shake flask to the bioreactor was successfully demonstrated in the scope of this work. The investigated process parameters included temperature, agitation and pH‐control. Especially the pH‐control improved the sporulation in the bioreactor when compared to shake flasks. The bioprocess resulted in a sporulation efficiency of 80%–90%. This corresponds to a sevenfold increase in sporulation efficiency due to a transfer to the bioreactor with pH‐control. Additionally, a design of experiment (DoE) was conducted to test the robustness of the bioprocess. This experiment validated the beforementioned sporulation efficiency for the developed bioprocess. Afterwards the bioprocess was then scaled up from a 1 L scale to a 10 L bioreactor scale. A comparable sporulation efficiency of 80% as in the small scale was achieved. The developed bioprocess facilitates the upscaling and application to an industrial scale, and can thus help meet the increasing market for probiotics.

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Facilitated endospore detection for Bacillus spp. through automated algorithm‐based image processing

Cited by 6 publications

References 35 publications

Antimicrobial Properties of Bacillus Probiotics as Animal Growth Promoters

Antimicrobial Properties of Bacillus Probiotics as Animal Growth Promoters

Digitalization concepts in academic bioprocess development

Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium

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