Facile synthesis of nano-nanocarriers from chitosan and pectin with improved stability and biocompatibility for anthocyanins delivery: An in vitro and in vivo study

Zhao, Xue; Zhang, Xuedi; Tie, Shanshan; Hou, Shuai; Wang, Haitao; Song, Yukun; Rai, Rewa; Tan, Mingqian

doi:10.1016/j.foodhyd.2020.106114

Cited by 90 publications

(50 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are in line with the previous report, showing that some polyphenols, such as blueberry polyphenols, tea polyphenols, and prunetin, can enhance the nematodes' resistance to the stress, which is reflected by the prolonged life span of C. elegans under heat stress and oxidative stress conditions [47,48]. Likewise, the heat-and oxidation-stress resistance of nematodes fed with chitosan-pectin nanocarriers and encapsulated with anthocyanins is significantly higher than those fed with free-state anthocyanin [49]. In in-vitro cellular assays, EGCG significantly reduces the damage of mesenchymal stem cells under heat stress and significantly improves the morphology and viability of the cells [50].…”

Section: Discussionsupporting

confidence: 92%

Construction and Application of EGCG-Loaded Lysozyme/Pectin Nanoparticles for Enhancing the Resistance of Nematodes to Heat and Oxidation Stresses

Lin

Cui

et al. 2021

Foods

View full text Add to dashboard Cite

Novel nanoparticles (NPs) were constructed with lysozyme (LY) and pectin (Ps) through self-assembly, which were used as a carrier to encapsulate epigallocatechin-3-gallate (EGCG). The binding of EGCG and LY is a static quenching process. Hydrogen bonds might play a major role in the formation of NPs, which has also been verified by a lower binding constant of EGCG with LY/Ps NPs. Meanwhile, EGCG could lead to conformational and microenvironmental changes of LY, resulting in more folding of LY secondary structures. In addition, attaching Ps to LY might inhibit LY aggregation induced by addition of free EGCG. At the LY/Ps mass ratio of 1:1, the constructed LY/Ps NPs had a high EGCG-loading capacity without a significant change in mean particle size, thus, our NPs could be used as an effective nanocarrier for loading EGCG. In vivo, compared with free EGCG, EGCG loaded onto LY/Ps NPs significantly increased Caenorhabditis elegans’ (C. elegans) resistance to heat stress and oxidative injury and prolonged their lifespan. This study provides theoretical basis and reference for constructing nanoactive substance carriers so as to improve the resistance of organisms to heat stress and oxidative damage and to increase their survival rate and extend their lifespan under environment stresses.

show abstract

Section: Discussionsupporting

confidence: 92%

Construction and Application of EGCG-Loaded Lysozyme/Pectin Nanoparticles for Enhancing the Resistance of Nematodes to Heat and Oxidation Stresses

Lin

Cui

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…The CC stretching vibration at 1610, 1557, and 1444 cm −1 from aromatic ring of PC significantly decreased after conjugation of 5-DTAF. 29,30 Moreover, the PCs had no green fluorescence, and F-PC showed an emission peak at 539 nm with a 13 nm red shift relative to the emission peak of 5-DTAF at 526 nm (Figure S2E). The UV−vis absorption spectrum of F-PC showed a peak at 280 nm for PCs (Figure S2F).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

pH-Responsive Core–Shell Microparticles Prepared by a Microfluidic Chip for the Encapsulation and Controlled Release of Procyanidins

Tie

Zhang

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to construct a delivery system using a microfluidic chip to protect procyanidins (PCs) and to achieve their pH-controlled release in simulated gastrointestinal fluid. The microfluidic chip was designed and fabricated to generate water-in-water-in-oil (W/W/O) templates for the preparation of sodium alginate/chitosan microparticles with a uniform size and core–shell structure, using an internal–external gelation method. Compared with free PCs, the stability of PCs embedded in microparticles was improved and a pH stimulus-responsive release of PCs from microparticles was observed under neutral pH conditions. The delivery system of microparticles was nontoxic and showed an inhibitory effect on the decrease of mitochondrial membrane potential in Caco-2 cells caused by H2O2 and acrylamide. This work provided a method for fabricating compact microfluidic chips to prepare a pH stimulus-responsive PCs delivery system with improved stability, which may have potential applications in the delivery of other nutrients.

show abstract

“…Another recent study reported the synthesis of anthocyanin‐loaded chitosan–pectin nanoparticles exploiting electrostatic interaction between the oppositely charged biopolymer. A higher molecular weight chitosan (300 kDa) was used in this study, and the reported EE was 66.68% 23 . The type of method and encapsulating materials preferred for the encapsulation process play a key role in obtaining a good EE.…”

Section: Resultsmentioning

confidence: 99%

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

et al. 2021

View full text Add to dashboard Cite

BACKGROUND Anthocyanins are flavonoids that are potential antioxidant, anti‐inflammatory, anti‐obesity, and anti‐carcinogenic nutraceutical ingredients. However, low chemical stability and low bioavailability limit the use of anthocyanins in food. Nanoencapsulation using biopolymers is a recent successful strategy for stabilization of anthocyanins. This study reports the development, characterization, and antioxidant activity of black carrot anthocyanin‐loaded chitosan nanoparticles (ACNPs). RESULTS The ionic gelation technique yielded the ACNPs. The mean hydrodynamic diameter d and polydispersity index PDI of chitosan nanoparticles and ACNPs were found to be d = 455 nm and PDI = 0.542 respectively for chitosan nanoparticles and d = 274 nm and PDI = 0.376 respectively for ACNPs. The size distribution was bimodal. The surface topography revealed that the ACNPs are spherical and display a coacervate structure. Fourier transform infrared analysis revealed physicochemical interactions of anthocyanins with chitosan. The loading process could achieve an encapsulation efficiency of 70%. The flow behavior index η of encapsulated ACNPs samples revealed Newtonian and shear thickening characteristics. There was a marginal reduction in the in vitro antioxidant potential of anthocyanins after nanoencapsulation, as evidenced from 2,2‐diphenyl‐1‐picrylhydrazyl, ferric reducing antioxidant power, and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) assays. Interestingly, the in vivo antioxidant potential of anthocyanins improved following nanoencapsulation, as observed in the serum antioxidant assays. CONCLUSION The optimized nanoencapsulation process resulted in spherical nanoparticles with appreciable encapsulation efficiency. The nanoencapsulation process improved the in vivo antioxidant activity of anthocyanins, indicating enhanced stability and bioavailability. The promising antioxidant activity of the ACNPs suggests a potential for utilization as a nutraceutical supplement. © 2021 Society of Chemical Industry.

show abstract

Facile synthesis of nano-nanocarriers from chitosan and pectin with improved stability and biocompatibility for anthocyanins delivery: An in vitro and in vivo study

Cited by 90 publications

References 50 publications

Construction and Application of EGCG-Loaded Lysozyme/Pectin Nanoparticles for Enhancing the Resistance of Nematodes to Heat and Oxidation Stresses

Construction and Application of EGCG-Loaded Lysozyme/Pectin Nanoparticles for Enhancing the Resistance of Nematodes to Heat and Oxidation Stresses

pH-Responsive Core–Shell Microparticles Prepared by a Microfluidic Chip for the Encapsulation and Controlled Release of Procyanidins

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

Contact Info

Product

Resources

About