Facile Synthesis of Hybrid Nanoflowers Using Glycine and Phenylalanine and Investigation of Their Catalytic Activity

Abstract

In the context of the proposed work, two different amino acids (Glycine, Phenylalanine) have interacted with copper ions in a phosphate buffer (PBS) in place of enzymes. This interaction resulted in the nucleation of copper phosphate crystals and the formation of flower‐shaped amino acid‐copper hybrid nanostructures (AA‐hNFs), which grew through self‐assembly. While Cu (II) ions in the structure of AA‐hNFs were used as Fenton's agent for the catalytic activity. SEM, energy dispersive X‐ray spectroscopy, X‐ray diffraction, and Fourier transform infrared spectroscopy measurements were used to define the AA‐hNFs′ characterisation. The peroxidase‐like activities of AA‐hNFs were investigated by UV/VIS spectrophotometer. Metal nanoparticles have peroxidase‐like activity. A class of enzymes known as peroxidases is able to catalyze the conversion of hydrogen peroxide into hydroxyl radicals. These radicals also take part in electron transfers with substrates, which results in color during oxidation. When cupric oxide nanoparticles are added to the peroxidase substrate while H2O2 is present, a blue color product with a maximum absorbance at=652 nm can result, demonstrating the catalytic activity of a peroxidase. The morphology and composition of AA‐hNFs were carefully characterized and the synthesized parameters were optimized systematically. Results showed that the nanoparticles were dispersed with an average diameter of 7–9 μm and indicated a uniform flower shape. The results of the investigation are anticipated to significantly advance a number of technical and scientific sectors.

Bioinspired Amino Acid Based Materials in Bionanotechnology: From Minimalistic Building Blocks and Assembly Mechanism to Applications

“…Enzymes play a crucial role in the metabolism of organisms and technological applications . Recent studies have shown that amino acids can coordinately self-assemble with metal ions that exhibited excellent catalytic activity similar to natural enzymes. − Makam et al synthesized a layered supramolecular amyloid-like ordered structure (Phe-Zn(II)) by assembling Phe with Zn 2+ in alkaline solution (Figure a), showing an excellent esterase catalytic efficiency of 76.54 M –1 s –1 , which exceeded the reported artificial biomolecular-based carbonic anhydrase mimetics. In addition, Phe-Zn(II) exhibited reversible catalysis of the hydration reaction of CO 2 in the presence of water, converting CO 2 into bicarbonate and protons, thus reducing the pH value .…”

Editorial: Enzyme immobilization technologies and their biomanufacturing applications

“…Recent years, many efforts have focused on this topic to develop biomolecule-inorganic hybrid nanoflowers with potential applications ( Cui and Jia, 2017 ). Demirbaş et al (2023) developed a facile synthesis method for hybrid nanoflowers using glycine and phenylalanine (AA-hNFs). The AA-hNFs exhibited a uniform flower shape and peroxidase-like activities.…”