Facile synthesis of bovine serum albumin conjugated low-dimensional ZnS nanocrystals

Mullamuri, Bhanu; Diwakar, Bhagavathula S.; Kasturi, Krishna C.S.B.; Reddy, Venu

doi:10.1016/j.ijbiomac.2017.03.164

Cited by 13 publications

(5 citation statements)

References 27 publications

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“…In the XRD pattern of GSH-ZnS NPs (Figure 1d), the diffraction patterns at 2θ = 29.14, 47.44, and 56.09°represented the (111), (220), and (311) planes of a cubic zinc blende phase of ZnS, respectively, which are in line with the literature. 31 Additionally, a characteristic ZnS plasmon band at 292 nm was observed in the UV−vis region of GSH-ZnS NPs, and a fluorescence peak appeared at 435 nm when excited with 305 nm (Figure 1e).…”

Section: Resultsmentioning

confidence: 96%

GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms

Zhou

Tong

Jiang

et al. 2020

ACS Appl. Bio Mater.

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Despite the good biocompatibility and antibacterial activity of zinc sulfide nanoparticles (ZnS NPs), whether they possess antiviral activity is still unclear. Here, GSHmodified ZnS NPs (GSH-ZnS NPs) were synthesized and their significant antiviral activity was demonstrated using the Arteriviridae family RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), as a model. Mechanistically, GSH-ZnS NPs were shown to reduce PRRSV-induced ROS production to prevent PRRSV multiplication, with no activating effect on the interferon (IFN) signal pathway, the first defense line against virus infection. Furthermore, isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis of GSH-ZnS NP-treated cells revealed the involvement of numerous crucial proteins in virus proliferation, with vitronectin (VTN) being confirmed as an efficient PRRSV antagonist here. Furthermore, GSH-ZnS NPs were found to have potent antiviral effects on the Herpesviridae family DNA virus, pseudorabies virus (PRV), the Coronaviridae family positive-sense RNA virus, porcine epidemic diarrhea virus (PEDV), and the Rhabdoviridae family negative-stranded RNA virus, vesicular stomatitis virus (VSV), indicating their broad-spectrum antiviral activity against viruses from different families with various genome types. Overall, GSH-ZnS NP is a prospective candidate for the development of antiviral nanomaterials and may serve as a model for investigation of potential host restriction factors in combination with proteomics.

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Section: Resultsmentioning

confidence: 96%

GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms

Zhou

Tong

Jiang

et al. 2020

ACS Appl. Bio Mater.

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“…The C-H vibrations at 3060 cm −1 were strongly suppressed in a sample of ZnS–BSA after milling and they completely disappeared in the ZnS–BSA after adsorption. In addition, more intense peaks in lower wavenumbers after the interaction of ZnS with BSA (e.g., at 1072 cm −1 , 986 cm −1 and 855 cm −1 ) could correspond to the interaction of Zn and BSA through the hydroxyl and amine groups present in BSA [ 16 ]. Moreover, as was mentioned above and also described in our previous paper [ 14 ], after the mechanochemical preparation of ZnS nanoparticles, the zeta potential of particles has a positive charge of up to 7.3, which is considerably higher than that found in the literature [ 41 , 42 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, albumin is the most abundant protein in blood plasma and plays a dominant role in controlling the distribution, excretion, therapeutic efficacy and toxicity of numerous endogenous and exogenous ligands in the body [ 15 ]. ZnS nanoparticles and nanocomposites coated with albumin were prepared by many researchers [ 16 , 17 , 18 , 19 ]. The interaction between these entities was also studied [ 20 , 21 ] with the evaluation of toxicity assessment [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy

Lukáčová Bujňáková,

Dutková,

Jakubíková

et al. 2023

Pharmaceuticals

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In this paper, ZnS nanoparticles were bioconjugated with bovine serum albumin and prepared in a form of nanosuspension using a wet circulation grinding. The stable nanosuspension with monomodal particle size distribution (d50 = 137 nm) and negative zeta potential (−18.3 mV) was obtained. The sorption kinetics and isotherm were determined. Interactions between ZnS and albumin were studied using the fluorescence techniques. The quenching mechanism, describing both static and dynamic interactions, was investigated. Various parameters were calculated, including the quenching rate constant, binding constant, stoichiometry of the binding process, and accessibility of fluorophore to the quencher. It has been found that tryptophan, in comparison to tyrosine, can be closer to the binding site established by analyzing the synchronous fluorescence spectra. The cellular mechanism in multiple myeloma cells treated with nanosuspension was evaluated by fluorescence assays for quantification of apoptosis, assessment of mitochondrial membrane potential and evaluation of cell cycle changes. The preliminary results confirm that the nontoxic nature of ZnS nanoparticles is potentially applicable in drug delivery systems. Additionally, slight changes in the secondary structure of albumin, accompanied by a decrease in α-helix content, were investigated using the FTIR method after analyzing the deconvoluted Amide I band spectra of ZnS nanoparticles conjugated with albumin. Thermogravimetric analysis and long-term stability studies were also performed to obtain a complete picture about the studied system.

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“…Magnetic nanoparticles, especially biocompatible Fe 3 O 4 (magnetite), can be easily separated from the matrix via an external magnetic field and target therapeutic agents 53 . Zinc sulfide nanoparticles, ZnS, are extensively studied semiconductor materials with optical properties that can be used in the production of luminescent NPs with multifunctional properties for novel diagnostic, therapeutic and imaging technologies in biomedicine 54 , 55 . When conjugated to drugs or diagnostic markers, FA retains its ability to bind to the folate receptor, which is overexpressed in most cancers, but not in healthy tissues 56 .…”

Section: Discussionmentioning

confidence: 99%

In vitro and ex vivo anti-myeloma effects of nanocomposite As4S4/ZnS/Fe3O4

Cholujová

Koklesová

Bujňáková

et al. 2022

Sci Rep

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Nanoparticles in medicine can integrate actively targeted imaging agents and drug delivery vehicles, and combining multiple types of therapeutics in a single particle has numerous advantages, especially in multiple myeloma. MM is an incurable hematological disorder characterized by clonal proliferation of plasma cells in the bone marrow. In this study, we evaluated the anti-myeloma activity of 3 nanocomposites (3NPs): As4S4/ZnS/Fe3O4 (1:4:1), As4S4/ZnS/Fe3O4 with folic acid (FA), and As4S4/ZnS/Fe3O4 with FA and albumin with reduced survival MM cell lines and primary MM samples by each of 3NP. Cytotoxic effects of 3NPs were associated with caspase- and mitochondria-dependent apoptosis induction and reduced c-Myc expression. Modulation of cell cycle regulators, such as p-ATM/ATM and p-ATR/ATR, and increases in p-Chk2, cyclin B1, and histones were accompanied by G2/M arrest triggered by 3NPs. In addition, 3NPs activated several myeloma-related signaling, including JNK1/2/3, ERK1/2 and mTOR. To overcome BM microenvironment-mediated drug resistance, nanocomposites retained its anti-MM activity in the presence of stroma. 3NPs significantly decreased the stem cell-like side population in MM cells, even in the context of stroma. We observed strong synergistic effects of 3NPs combined with lenalidomide, pomalidomide, or melphalan, suggesting the potential of these combinations for future clinical studies.

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Facile synthesis of bovine serum albumin conjugated low-dimensional ZnS nanocrystals

Cited by 13 publications

References 27 publications

GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms

GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms

Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy

In vitro and ex vivo anti-myeloma effects of nanocomposite As4S4/ZnS/Fe3O4

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