Facile synthesis of an aminopropylsilane layer on Si/SiO2 substrates using ethanol as APTES solvent

Miranda, Adelaide; Martínez, Lidia; Beule, Pieter De

doi:10.1016/j.mex.2020.100931

Cited by 32 publications

(27 citation statements)

References 50 publications

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“…The increased intensity of the major peak suggests that more carbon elements were found on the surface, which supported the longer linkers immobilized along with the immobilization steps. Other than the nude silicon wafer, minor peaks were observed at the binding energy range of 287.5 eV to 288.5 eV after APTES immobilization, which may be attributed to C-N/C-O/C=O [ 37 , 38 ]. Figure 2 B shows the XPS spectrum of nitrogen element of four surface modifications as aforementioned.…”

Section: Resultsmentioning

confidence: 99%

Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

et al. 2021

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The prevalence of hepatitis B virus (HBV) is a global healthcare threat, particularly chronic hepatitis B (CHB) that might lead to hepatocellular carcinoma (HCC) should not be neglected. Although many types of HBV diagnosis detection methods are available, some technical challenges, such as the high cost or lack of practical feasibility, need to be overcome. In this study, the polycrystalline silicon nanowire field-effect transistors (pSiNWFETs) were fabricated through commercial process technology and then chemically functionalized for sensing hepatitis B virus surface antigen (HBsAg) and hepatitis B virus X protein (HBx) at the femto-molar level. These two proteins have been suggested to be related to the HCC development, while the former is also the hallmark for HBV diagnosis, and the latter is an RNA-binding protein. Interestingly, these two proteins carried opposite net charges, which could serve as complementary candidates for evaluating the charge-based sensing mechanism in the pSiNWFET. The measurements on the threshold voltage shifts of pSiNWFETs showed a consistent correspondence to the polarity of the charges on the proteins studied. We believe that this report can pave the way towards developing an approachable tool for biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

et al. 2021

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“…APTES is a common organosilane of surface modification on silicon oxide surfaces. Because of the amino group, APTES can be used as an intermediate layer . Some research studies have shown that APTES in ethanol can form multilayers on oxide surfaces .…”

Section: Methodsmentioning

confidence: 99%

“…Because of the amino group, APTES can be used as an intermediate layer. 62 Some research studies have shown that APTES in ethanol can form multilayers on oxide surfaces. 62 In our work, ethanol was used as the APTES solvent to functionalize silicon dioxide surface, and this process can be carried out at room temperature.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Green Synthesis-Based Magnetic Diatoms for Biological Applications

Dao

Ankireddy

Zhao

et al. 2021

ACS Sustainable Chem. Eng.

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Here, we report the green synthesis of silica-based materials for the treatment of biological samples such as bacteria enrichment and exosome isolation. The synthesized materials were nanosized, recyclable, and magnetically separable using an eco-friendly hydrothermal synthesis approach. Fe 3 O 4 formation on the surface of biosilica porous diatomite via precipitation was used to create a magnetic diatom (mag-DE) through a bottom-up approach, which was low cost, robust, nontoxic, and eco-friendly. Following the addition of L-arginine-containing amino groups, the mag-DE showed broad absorbance in the visible light spectrum (UV−vis), and the amine functional groups of 3-aminopropyl triethoxysilane on the DE via the amidine bond were confirmed using Fourier transform infrared analysis. Further studies were conducted by conjugating the mag-DE with concanavalin A (ConA), a known micro-antimicrobial agent for bacterial enrichment, as well as with a specific antibody for exosome isolation. The large-volume samples obtained were easy to use, and the materials could enrich a minimum of 50 colony-forming units (CFUs) of Salmonella and also showed a promising ability to capture another bacterial species. In addition, the modified synthetic mag-DE could be used for exosome isolation using affinity purification. Functionalized mag-DE could be magnetically isolated in a rapid and more efficient manner than using the conventional method, which can be altered for exosome isolation in research and clinical applications. This study presents the unique physical and chemical properties of green synthesized mag-DE, whose surface can be easily modified. Thus, it creates a novel potential strategy for several desirable applications for various types of biological samples.

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“…In a second step, APTES was covalently attached to the TEOS surface of both NPs and substrates by incubating the materials in the respective sol-gel medium. The experimental procedure described in the Methods section should ensure that multilayer formation of APTES is suppressed due to the short reaction time of pre-hydrolyzed APTES in a diluted alcoholic solvent at elevated temperatures followed by rinsing with acetic acid [34,35,54,55]. The topographical surface roughness of the dip-coated planar substrates is evaluated using AFM and SEM as shown in Fig.…”

Section: Mimicking the Nanoparticle Surface On Smooth And Rough Plana...mentioning

confidence: 99%

Assessment of nanoparticle immersion depth at liquid interfaces from chemically equivalent macroscopic surfaces

Smits

Giri

Shen

et al. 2022

Journal of Colloid and Interface Science

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Facile synthesis of an aminopropylsilane layer on Si/SiO2 substrates using ethanol as APTES solvent

Cited by 32 publications

References 50 publications

Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

Green Synthesis-Based Magnetic Diatoms for Biological Applications

Assessment of nanoparticle immersion depth at liquid interfaces from chemically equivalent macroscopic surfaces

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