Facile synthesis of a highly SERS active nanosilver sol using microwaves and its application in the detection of E. coli using Victoria blue B as a molecular probe

Wang, Yaohui; Zhang, Xinghui; Wen, Guiqing; Liang, Aihui; Jiang, Zhiliang

doi:10.1039/c6ay00728g

Cited by 12 publications

(4 citation statements)

References 32 publications

(38 reference statements)

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“…However, the spectral information of these three pathogens is not completely coincident with other studies. Comparing with other studies (Xie et al, 2013; Wang et al, 2016), they appear to be different peaks. It indicates that these spectra only presented ingredient information activated by metallic nanosilver.…”

Section: Discussioncontrasting

confidence: 62%

“…Compared with silver, gold is more expensive but produces weaker SERS enhancement than silver (Fan et al, 2011; Chuang et al, 2014). In addition, nanosilver has the following advantages: a high molar extinction coefficient, excellent optical properties and nanosilver aggregates having strong SERS effects (Wang et al, 2016). Therefore, the silver colloidal nanoparticles (AgNPs) were employed widely for bacteria detection.…”

Section: Introductionmentioning

confidence: 99%

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Surface-Enhanced Raman Scattering (SERS) With Silver Nano Substrates Synthesized by Microwave for Rapid Detection of Foodborne Pathogens

Wei

Zhao

2018

Front. Microbiol.

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Rapid and sensitive methods have been developed to detect foodborne pathogens, a development that is important for food safety. The aim of this study is to explore Surface-enhanced Raman scattering (SERS) with silver nano substrates to detect and identify the following three foodborne pathogens: Escherichia coli O157: H7, Staphylococcus aureus and Salmonella. All the cells were resuspended with 10 mL silver colloidal nanoparticles, making a concentration of 107 CFU/mL, and were then exposed to 785 nm laser excitation. In this study, the results showed that all the bacteria can be sensitively and reproducibly detected directly by SERS. The distinctive differences can be observed in the SERS spectral data of the three food-borne pathogens, and the silver colloidal nanoparticles can be used as highly sensitive SERS-active substrates. In addition, the assay time required only a few minutes, which indicated that SERS coupled with the silver colloidal nanoparticles is a promising method for the detection and characterization of food-borne pathogens. At the same time, principle component analysis (PCA) and hierarchical cluster analysis (HCA) made the different bacterial strains clearly differentiated based on the barcode spectral data reduction. Therefore, the SERS methods hold great promise for the detection and identification of food-borne pathogens and even for applications in food safety.

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Section: Discussioncontrasting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Scattering (SERS) With Silver Nano Substrates Synthesized by Microwave for Rapid Detection of Foodborne Pathogens

Wei

Zhao

2018

Front. Microbiol.

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“…SERS is a label-free and highly sensitive method for detecting and analyzing biological and chemical analytes. − Silver nanoparticles (AgNPs) have been commonly used as the SERS substrate because of their high molar extinction coefficient and excellent optical properties. , Usually, the highly organized and dense AgNPs arrays can enable the highly sensitive SERS sensing, and thereby one in situ method for growing AgNPs in the detection zone of the NanoPADs was used. The design of the SERS-NanoPADs is shown in Figure a, and it consists of two microchannels that meet in the detection zone (circular area labeled in Figure bi).…”

Section: Resultsmentioning

confidence: 99%

Facile Microembossing Process for Microchannel Fabrication for Nanocellulose-Paper-Based Microfluidics

Yuan

Jiao

et al. 2023

ACS Appl. Mater. Interfaces

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Nanofibrillated cellulose paper (nanopaper) has gained growing interest as one promising substrate material for paper-based microfluidics, thanks to its ultrasmooth surface, high optical transparency, uniform nanofiber matrix with nanoscale porosity, and tunable chemical properties. Recently, research on nanopaper-based microfluidics has quickly advanced; however, the current technique of patterning microchannels on nanopaper (i.e., 3D printing, spray coating, or manual cutting and sticking), that is fundamental for application development, still has some limitations, such as ease-of-contamination, and more importantly, only enabling millimeter-scale channels. This paper reports a facile process that leverages the simple operations of microembossing with the convenient plastic micro-molds, for the first time, patterning nanopaper microchannels downing to 200 μm, which is 4 times better than the existing methods and is time-saving (<45 mins). We also optimized the patterning parameters and provided one quick look-up table as the guideline for application developments. As proof-of-concept, we first demonstrated two fundamental microfluidic devices on nanopaper, the laminar-mixer and droplet generator, and two functional nanopaper-based analytical devices (NanoPADs) for glucose and Rhodamine B (RhB) sensing based on optical colorimetry and surface-enhanced Raman spectroscopy, respectively. The two NanoPADs showed outstanding performance with low limits of detection (2 mM for glucose and 19fM for RhB), which are 1.25× and 500× fold improvement compared to the previously reported values. This can be attributed to our newly developed highly accurate microchannel patterning process that enables high integration and fine-tunability of the NanoPADs along with the superior optical properties of nanopaper.

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“…Silver and gold nanoparticles are commonly used nanomaterials for the preparation of SEIRA and SERS substrates, but gold nanoparticles are more expensive than silver nanoparticles and they produce weaker LSPR enhancement [ 56 – 60 ]. Moreover, silver nanoparticles have a higher molar extension coefficient, form metal islands that have a strong SEIRA and SERS effect, have a simple one-step synthesis procedure, and have excellent optical properties [ 56 , 61 – 63 ] so we employed silver nanoparticles in the proposed method.…”

Section: Resultsmentioning

confidence: 99%

Identification of milk quality and adulteration by surface-enhanced infrared absorption spectroscopy coupled to artificial neural networks using citrate-capped silver nanoislands

2022

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Milk is one of the most important multicomponent superfoods owing to its rich macronutrient composition. It requires quality control at all the production stages from the farm to the finished products. A localized surface plasmon resonance optical sensor based on a citrate-capped silver nanoparticle (Cit-AgNP)–coated glass substrate was developed. The fabrication of such sensors involved a single-step synthesis of Cit-AgNPs followed by surface modification of glass slides to be coated with the nanoparticles. The scanning electron microscope micrographs demonstrated that the nanoparticles formed monolayer islands on glass slides. The developed surface-enhanced infrared absorption spectroscopy (SEIRA) sensor was coupled to artificial neural networking (ANN) for the qualitative differentiation between cow, camel, goat, buffalo, and infants’ formula powdered milk types. Moreover, it can be used for the quantitative determination of the main milk components such as fat, casein, urea, and lactose in each milk type. The qualitative results showed that the obtained FTIR spectra of cow and buffalo milk have high similarity, whereas camel milk resembled infant formula powdered milk. The most difference in FTIR characteristics was evidenced in the case of goat milk. The developed sensor adds several advantages over the traditional techniques of milk analysis using MilkoScan™ such as less generated waste, elimination of pre-treatment steps, minimal sample volume, low operation time, and on-site analysis. Graphical abstract

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Facile synthesis of a highly SERS active nanosilver sol using microwaves and its application in the detection of E. coli using Victoria blue B as a molecular probe

Cited by 12 publications

References 32 publications

Surface-Enhanced Raman Scattering (SERS) With Silver Nano Substrates Synthesized by Microwave for Rapid Detection of Foodborne Pathogens

Surface-Enhanced Raman Scattering (SERS) With Silver Nano Substrates Synthesized by Microwave for Rapid Detection of Foodborne Pathogens

Facile Microembossing Process for Microchannel Fabrication for Nanocellulose-Paper-Based Microfluidics

Identification of milk quality and adulteration by surface-enhanced infrared absorption spectroscopy coupled to artificial neural networks using citrate-capped silver nanoislands

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