Facile recovery of acetic acid from waste acids of electronic industry via a partial neutralization pretreatment (PNP) – Distillation strategy

Ni, Chong; Wu, Xiang; Jin, Dan; Du, Dongyun

doi:10.1016/j.seppur.2014.04.052

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…Acetate (C 2 H 4 O 2 ) is one of the simple weak acids, which can be widely produced from syngas via chemical [1] or microbial [2] catalysis or generated from hydrolysis or pyrolysis of the cellulosic biomass [3]. Acetate is also a product of anaerobic fermentation in treated wastewater [4].…”

Section: Introductionmentioning

confidence: 99%

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Bai

Liu

et al. 2019

Biotechnol Biofuels

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BackgroundAcetate, an economical industrial chemical, which is also the precursor of acetyl-CoA, could serve as an alternative substrate for biomanufacturing. This nontraditional substrate can be widely produced from syngas via hydrolysis or pyrolysis of the cellulosic biomass, chemical or microbial catalysis, anaerobic fermentation in treated wastewater, etc. However, the toxicity of acetate to microorganisms has held back its utilization, especially for the eukaryotes that are good hosts for production of complicated pharmaceuticals or chemicals. This study seeks to improve acetate utilization in a widely used yeast host, Komagataella phaffii (previously Pichia pastoris), by metabolic engineering of acetate tolerance, transport, and metabolism.ResultsA kinase-deficient library of K. phaffii was firstly used to screen acetate-resistant kinases. The HRK1 knockout strain was sensitive to acetate and overexpression of this gene improved acetate tolerance and cell growth of the strain. Also, overexpression of HRK1 caused a 55% productivity improvement of acetyl-CoA-dependent 6-methylsalicylic acid (6-MSA). However, activation of Hrk1 on membrane H(+)-ATPase Pma1 seemed not to work in the engineered strain. Acetate transporter gene ScFPS1* was further overexpressed, despite of not improving 6-MSA biosynthesis. To enhance acetate metabolism, acetyl-CoA synthesizing related genes, yeast PpACS1, ScACS1*, and E. coli ackA/pta were overexpressed separately. Introduction of PpACS1 and ScACS1* each increased biosynthesis of 6-MSA by approximately 20% on 20 mM acetate. Finally, co-overexpression of HRK1 and ScACS1* improved 6-MSA productivity by 51% on 20 mM acetate, despite that a low expression level of HRK1 happened when genes were expressed under the same promoter.ConclusionsHRK1 screened by K. phaffii kinase-deficient library played an important role in acetate tolerance and was proved to profit the biosynthesis of acetyl-CoA-derived chemicals. It could be a potential target for metabolic engineering of acetate utilization in other eukaryotic hosts as well. A combined strategy of introducing genes for acetate tolerance and metabolism further improved biosynthesis of acetyl-CoA derived reporter compound in K. phaffii. This makes it a good choice for acetyl-CoA-derived chemicals with acetate as substrate or precursor in K. phaffii, which would also extend the use of this chassis host.Electronic supplementary materialThe online version of this article (10.1186/s13068-019-1404-0) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Bai

Liu

et al. 2019

Biotechnol Biofuels

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“…Acetate has been considered as a potential low-cost carbon source, since it could be generated in both biological and chemical processes, including acidogenesis, acetogenesis, methanogenesis and syngas fermentation. [4][5][6][7] Zhang et al successfully produced phloroglucinol from acetate by in vitro reaction. 8 However, the costings and nonrecycling of the enzymes used in vitro were the main obstacles.…”

Section: Introductionmentioning

confidence: 99%

Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli

Xian

Liu

2017

RSC Adv.

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“…In order to reduce the cost of the acid modified sorbent instead of synthetic acids, waste acids should be used as previously reported by Zhao et al [13], Li et al [18], Du et al [19].…”

Section: Figure 2 Cao Conversion Of Sorbents Modified By Acids Undermentioning

confidence: 99%

Modification of Waste-derived CaO Using Organic Acids for CO<sub>2</sub> Capture

Nawar¹,

Ali²,

Khan³

et al. 2019

IJEEE

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Calcium looping is a widely used CO 2 capture technology, where calcium oxide (CaO) is used as a sorbent. However, it has many drawbacks such as expensive raw material and reduction in sorbent capacity over multiple cycles. This study is aimed at developing economic as well as environment friendly sorbents for CO 2 capturing. For this purpose, chicken eggshells were collected from household municipal waste as a CaO sorbent for CO 2 capture. The eggshell sorbent was characterized using different techniques such as SEM-EDS, XRD and TGA. Three different organic acids were used to improve the conversion of eggshell sorbents i.e., lactic, oxalic and tartaric acid. The results showed that one out of three acids i.e., lactic acid (ES LA-10%) showed improved conversion and stability over a period of 20 cycles as compared to other acids. In terms of CaO conversion ES LA-10% displayed the maximum performance of 47.8% and had improved cyclic stability during 20 cycles. Hence, this study showed that modifying sorbent (eggshells) by using acid is a better sorbent in comparison with other natural and synthetic sorbent, therefore reducing waste and cost simultaneously.

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Facile recovery of acetic acid from waste acids of electronic industry via a partial neutralization pretreatment (PNP) – Distillation strategy

Cited by 14 publications

References 17 publications

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli

Modification of Waste-derived CaO Using Organic Acids for CO<sub>2</sub> Capture

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Facile recovery of acetic acid from waste acids of electronic industry via a partial neutralization pretreatment (PNP) – Distillation strategy

Cited by 14 publications

References 17 publications

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli

Modification of Waste-derived CaO Using Organic Acids for CO&lt;sub&gt;2&lt;/sub&gt; Capture

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Modification of Waste-derived CaO Using Organic Acids for CO<sub>2</sub> Capture