Facile production of quercetin nanoparticles using 3D printed centrifugal flow reactors

Grandi, Davide De; Meghdadi, Alireza; LuTheryn, Gareth; Carugo, Dario

doi:10.1039/d2ra02745c

Cited by 4 publications

(3 citation statements)

References 100 publications

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“…Importantly, the transition temperature of a lipid (i.e., the temperature at which the lipid transitions from the ordered gel phase to the disordered liquid crystalline phase) determines the rigidity of a lipid bilayer and can impact the physical properties and stability of the formed liposomes [34]. Moreover, liposomal formulations investigated in previous research often In previous research, we showed that the RIAC is capable of producing liposomes (as well as inorganic nanoparticles and drug nanocrystals) at a relatively low cost and without requiring specialist instrumentation [25,30]. These earlier proof-of-concept studies, however, employed model lipids that are less commonly used clinically for therapeutic purposes [31,32].…”

Section: Introductionmentioning

confidence: 99%

“…In previous research, we showed that the RIAC is capable of producing liposomes (as well as inorganic nanoparticles and drug nanocrystals) at a relatively low cost and without requiring specialist instrumentation [ 25 , 30 ]. These earlier proof-of-concept studies, however, employed model lipids that are less commonly used clinically for therapeutic purposes [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…The lipidic solution and water are separately added to the reservoirs and, upon actuation of the centrifuge, they are driven through the spiral channel where they rapidly mix with each other, leading to lipid selfassembly into liposomes. In previous research, we showed that the RIAC is capable of producing liposomes (as well as inorganic nanoparticles and drug nanocrystals) at a relatively low cost and without requiring specialist instrumentation [25,30]. These earlier proof-of-concept studies, however, employed model lipids that are less commonly used clinically for therapeutic purposes [31,32].…”

Section: Introductionmentioning

confidence: 99%

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Rapid Production of Nanoscale Liposomes Using a 3D-Printed Reactor-In-A-Centrifuge: Formulation, Characterisation, and Super-Resolution Imaging

He,

Grandi,

Chandradoss

et al. 2023

Micromachines

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Nanoscale liposomes have been extensively researched and employed clinically for the delivery of biologically active compounds, including chemotherapy drugs and vaccines, offering improved pharmacokinetic behaviour and therapeutic outcomes. Traditional laboratory-scale production methods often suffer from limited control over liposome properties (e.g., size and lamellarity) and rely on laborious multistep procedures, which may limit pre-clinical research developments and innovation in this area. The widespread adoption of alternative, more controllable microfluidic-based methods is often hindered by complexities and costs associated with device manufacturing and operation, as well as the short device lifetime and the relatively low liposome production rates in some cases. In this study, we demonstrated the production of liposomes comprising therapeutically relevant lipid formulations, using a cost-effective 3D-printed reactor-in-a-centrifuge (RIAC) device. By adjusting formulation- and production-related parameters, including the concentration of polyethylene glycol (PEG), temperature, centrifugation time and speed, and lipid concentration, the mean size of the produced liposomes could be tuned in the range of 140 to 200 nm. By combining selected experimental parameters, the method was capable of producing liposomes with a therapeutically relevant mean size of ~174 nm with narrow size distribution (polydispersity index, PDI ~0.1) at a production rate of >8 mg/min. The flow-through method proposed in this study has potential to become an effective and versatile laboratory-scale approach to simplify the synthesis of therapeutic liposomal formulations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid Production of Nanoscale Liposomes Using a 3D-Printed Reactor-In-A-Centrifuge: Formulation, Characterisation, and Super-Resolution Imaging

He,

Grandi,

Chandradoss

et al. 2023

Micromachines

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Chitosan-Coated Nanoliposomes: Exploring the Impact on Physicochemical Properties, Stability, Antioxidant Activity, and Molecular Characterization of Chlorella-Peptide Fractions

Gharehbeglou,

Sarabandi,

Akbarbaglu

2024

J Polym Environ

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Densely Packed Chemical Synthesis Equipment by 3D Spatial Design and Additive Manufacturing: Acetylene Generation Cartridge

Erokhin

Ananikov

2023

Org. Process Res. Dev.

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Preparation of and carrying out chemical reactions often require considerable laboratory space. Miniaturization of chemical equipment and reducing laboratory space requirements are an essential task to improve cost-efficiency, as well as increase safety and decrease potential risks. In this work, we discuss the miniaturization of laboratory equipment through the spatial optimization of functional parts. To demonstrate this, we have optimized the size of one of the most cumbersome processes in the lab, the generation of gases from solid sources. We have developed a ready-to-use acetylene generation cartridge with a high degree of functional filling of the internal space, which significantly compacts its dimensions. The cartridge was sealed upon three-dimensional (3D) printing, prepacked with the reagent, and found suitable for long-term storage. In addition to acetylene generation, built-in water trap channels and a drying compartment made it possible to obtain dried gas on the output of the cartridge. The cartridge showed high efficiency in the reactions for the synthesis of bis(arylthio)-substituted ethenes and butadienes. To expand the range of products based on acetylene, a procedure for the synthesis of 1,2-bis(alkylthio)ethenes was developed. The use of the cartridge allowed us to obtain target Z-ethenes with good yields and stereoselectivity.

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Facile production of quercetin nanoparticles using 3D printed centrifugal flow reactors

Abstract: A 3D printed reactor-in-a-centrifuge (RIAC) was developed to produce drug nanocrystals. Quercetin nanocrystals were manufactured at varying operational and formulation conditions, and had a small size (190–302 nm) and low size dispersity (PDI < 0.1).

Cited by 4 publications

References 100 publications

Rapid Production of Nanoscale Liposomes Using a 3D-Printed Reactor-In-A-Centrifuge: Formulation, Characterisation, and Super-Resolution Imaging

Rapid Production of Nanoscale Liposomes Using a 3D-Printed Reactor-In-A-Centrifuge: Formulation, Characterisation, and Super-Resolution Imaging

Chitosan-Coated Nanoliposomes: Exploring the Impact on Physicochemical Properties, Stability, Antioxidant Activity, and Molecular Characterization of Chlorella-Peptide Fractions

Densely Packed Chemical Synthesis Equipment by 3D Spatial Design and Additive Manufacturing: Acetylene Generation Cartridge

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