Facile preparation of ordered mesoporous silica–carbon composite nanoparticles for glycan enrichment

Qin, Hongqiang; Hu, Zhengyan; Wang, Fangjun; Zhang, Yi; Zhao, Liang; Xu, Guiju; Wu, Ren’an; Zou, Hanfa

doi:10.1039/c3cc41479e

Cited by 49 publications

(38 citation statements)

References 26 publications

(4 reference statements)

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“…Besides endogenous peptidome analysis, size-exclusive effect of mesoporous materials was also employed for selective extraction of other LMW species, including glycans [137][138][139] and lipids [80], in the presence of interference HMW proteins. Zou et al [137] prepared oxidized mesoporous carbon for selective enrichment of N-linked glycans after PNGase F cleavage in the presence of residual proteins, and 5 N-linked glycans were identified as potential biomarkers of liver cancer.…”

Section: Imprinted Polymersmentioning

confidence: 99%

“…Zou et al [137] prepared oxidized mesoporous carbon for selective enrichment of N-linked glycans after PNGase F cleavage in the presence of residual proteins, and 5 N-linked glycans were identified as potential biomarkers of liver cancer. The oxidized mesoporous carbon was replaced by mesoporous silica-carbon composite nanoparticles NP-MCM-C with mesoporous silica support and carbon modified inner surface to obtain uniform particle shape and better dispersity, and a more sensitive and comprehensive glycan enrichment was accomplished [138].…”

Section: Imprinted Polymersmentioning

confidence: 99%

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Recent advances in applications of nanomaterials for sample preparation

et al. 2016

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Section: Imprinted Polymersmentioning

confidence: 99%

Section: Imprinted Polymersmentioning

confidence: 99%

Recent advances in applications of nanomaterials for sample preparation

et al. 2016

Talanta

123

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“…The graphitized carbon deposited on the inner surface of the mesoporous silica shell yields a large graphitized hydrophobic inner surface area 30 and strong UV adsorption. 31 The outer hydrophilic mesoporous silica shell guarantees good dispersibility of the Au@GMSN.…”

Section: ■ Introductionmentioning

confidence: 99%

Facile Synthesis of Gold@Graphitized Mesoporous Silica Nanocomposite and Its Surface-Assisted Laser Desorption/Ionization for Time-of-Flight Mass Spectroscopy

Liu

Peng

et al. 2015

ACS Appl. Mater. Interfaces

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In this work, a novel core-shell structured gold@graphitized mesoporous silica nanocomposite (Au@GMSN) was synthesized by in situ graphitization of template within the mesochannels of mesoporous silica shell on gold core and demonstrated to be promising nanomaterials for surface-assisted laser desorption/ionization time-of-flight mass spectroscopy (SALDI-TOF MS). The integration of the graphitized mesoporous silica with the gold nanoparticles endowed Au@GMSN with large surface areas of graphitic structure, good dispersibility, and strong ultraviolet (UV) absorption. Au@GMSN exerted the synergistic effect on the efficient detection of small-molecular-weight analytes including amino acids, neutral saccharides, peptides, and traditional Chinese medicine. The Au@GMSN-assisted laser desorption/ionization exhibited the following superiorities: high ionization efficiency, low fragmentation interference, favorable salt tolerance, and good reproducibility. Moreover, because of the large hydrophobic inner surface area of the graphitized mesoporous silica shell, the Au@GMSN demonstrated its promising capacity in the pre-enrichment of aromatic analytes prior to SALDI-TOF MS, which favored rapid and sensitive detection.

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“…To address the issues in detection of the N-glycans by MS, a variety of glycan purification approaches have been developed, including lectins enrichment [6], hydrophilic interaction liquid chromatography (HILIC) [7], porous graphitized carbon (PGC) columns/tips [8] or porous active carbon purification [9] and reversible bond formation [10,11]. In these strategies, special care is required when working with these solid phases, as their ability to strongly adsorb a variety of polar substances may lead to low selectivity of glycans.…”

Section: Introductionmentioning

confidence: 99%

Highly specific purification of N-glycans using phosphate-based derivatization as an affinity tag in combination with Ti4+-SPE enrichment for mass spectrometric analysis

Zhang

Bin

et al. 2016

Analytica Chimica Acta

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N-linked protein glycosylation is involved in regulation of a wide variety of cellular processes and associated with numerous diseases. Highly specific identification of N-glycome remains a challenge while its biological significance is acknowledged. The relatively low abundance of glycan in complex biological mixtures, lack of basic sites for protonation, and suppression by other highly abundant proteins/peptides lead to the particularly poor detection sensitivity of N-glycans in the MS analysis. Therefore, the highly specific purification procedure becomes a crucial step prior to MS analysis of the N-glycome. Herein, a novel N-glycans enrichment approach based on phosphate derivatization combined with Ti(4+)-SPE (solid phase extraction) was developed. Briefly, in this strategy, N-glycans were chemically labeled with a phospho-group at their reducing ends, such that the Ti(4+)-SPE microspheres were able to capture the phospho-containing glycans. The enrichment method was developed and optimized using model oligosaccharides (maltoheptaose DP7 and sialylated glycan A1) and also glycans from a standard glycoprotein (asialofetuin, ASF). This method experimentally showed high derivatization efficiency (almost 100%), excellent selectivity (analyzing DP7 in the digests of bovine serum albumin at a mass ratio of 1:100), high enriching recovery (90%), good reproducibility (CV<15%) as well as high sensitivity (LOD at fmol level). At last, the proposed method was successfully applied in the profiling of N-glycome in human serum, in which a total of 31 N-glycan masses were identified.

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Facile preparation of ordered mesoporous silica–carbon composite nanoparticles for glycan enrichment

Cited by 49 publications

References 26 publications

Recent advances in applications of nanomaterials for sample preparation

Recent advances in applications of nanomaterials for sample preparation

Facile Synthesis of Gold@Graphitized Mesoporous Silica Nanocomposite and Its Surface-Assisted Laser Desorption/Ionization for Time-of-Flight Mass Spectroscopy

Highly specific purification of N-glycans using phosphate-based derivatization as an affinity tag in combination with Ti4+-SPE enrichment for mass spectrometric analysis

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