Facile preparation of magnetically functionalized graphite nanosheets for porcine pancreatic lipase immobilization

Abstract

The graphite nanosheets decorated with Fe 3 O 4 nanoparticles (about 15-20 nm), i.e., Fe 3 O 4 @graphite nanosheets, have been prepared via a facile one-pot method. The resulting composites with large specific surface area, high affinity, and rapid magnetic response were characterized by means of Fourier transform infrared spectra, Raman spectra, powder X-ray diffraction, scanning electron microscopy, transmission electron microscopy, elemental analysis, and thermogravimetric analysis, which indicated that the Fe 3 O 4 nanoparticles were chemically integrated on the graphite nanosheets. The porcine pancreatic lipase (PPL) was immobilized onto the Fe 3 O 4 @graphite nanosheets through covalent bonding using glutaraldehyde as a coupling reagent, and the properties of the resulting immobilized PPL such as enzymatic activity, stability, and reusability were researched at the same time. The result proved that the immobilized PPL displayed excellent properties and demonstrated that the prepared Fe 3 O 4 @graphite nanosheets would present a potential application as a novel carrier for enzyme immobilization, enrichment, and separation.

Robust and Recyclable Two-Dimensional Nanobiocatalysts for Biphasic Reactions in Pickering Emulsions

“…The lipase loading percent values for GO, P-rGO, and P-rGO/ZIF-8 (mass ratio of ZIF-8 to P-rGO was 1.5:1) were 104.06, 64.25, and 40.33%, respectively (Figure b). We further compared our 2D nanobiocatalysts with other catalysts, such as membranes, , CRGO, and Fe 3 O 4 decorated RGO . When utilized for enzyme immobilization, 2D polymer membranes usually possessed relatively lower enzyme loading percent, which was owing to the low surface area.…”