Facile Preparation of Hydrophilic Mesoporous Metal–Organic Framework via Synergistic Etching and Surface Functionalization for Glycopeptides Analysis

Abstract

In view of the size and hydrophilicity of glycopeptides, materials having suitable channels (size-exclusion) and strong hydrophilic surface (hydrophilic interaction) are preferred to enrich the glycopeptides in biological samples. Metal–organic frameworks (MOFs) are good candidates. However, their smaller microporous channels and low chemical stability have limited the application. Herein, a facile strategy was established to construct hydrophilic mesoporous MOF via synergistic etching and surface functionalization by using phytic acid (PA). Besides, polyvinylpyrrolidone (PVP) was added during MOF synthesis to enhance the water stability of the MOF. Owing to the expanded hydrophilic mesoporous channels, the PA-modified Ce-MOF effectively and selectively captured 422 glycopeptides from 155 glycoproteins in tryptic digests of human serum (2 μL). The present work sheds light on the easy fabrication of hydrophilic mesoporous materials, and this established material holds unique advantages for glycopeptides analysis in biological samples.

Fabrication of magnetic dual-hydrophilic metal organic framework for highly efficient glycopeptide enrichment

“…The fitted subpeaks of P2p (ESM Fig. S1c) were attributed to the contributions of Zr-O-P (134.2 eV) and R-PO 3 H 2 (133.3 eV), which indicated the presence of two chemical environments for phosphorus atoms [31,32]. XPS analysis clearly confirmed the coordination interaction between PA molecules and Zr 4+ .…”

A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry–Based Glycoproteomics

“…A common property achieved by these materials is hydrophilicity, although the avenues to achieve such properties differ. Materials include cobalt sulfide nanoleaves ( 242 ), covalent/metal organic framework approaches ( 181 , 243 , 244 , 245 , 246 ), carbon microspheres ( 247 ), and polymeric monoliths functionalized with piperazine ( 248 ), among others. As described above, some of these materials can have bifunctional properties to enable HILIC-based glycopeptide enrichment and metal ion–based phosphopeptide enrichment ( 181 , 182 , 249 ).…”

Advances in mass spectrometry‐based glycoproteomics: An update covering the period 2017–2021

“…Properties of MOFs such as a large surface area and adjustable pore sizes make them suitable for use in the enrichment of glycopeptides [84,89]. Pu et al [90] used phytic acid (PA) to synthesize the hydrophilic mesoporous ceriumbased MOF (Ce-MOF@PA). The microporosity increments and the chemical stability of the novel MOF increased the number of enriched glycopeptides with respect to HILIC, as shown in Figure 3A(1-3).…”