Facile preparation of fluorescent Au nanoclusters-based test papers for recyclable detection of Hg2+ and Pb2+

Bian, Ruixin; Wu, Xiaotong; Chai, Fang; Li, Lü; Zhang, Lingyu; Wang, Tingting; Wang, Chungang; Su, Zhong‐Min

doi:10.1016/j.snb.2016.10.120

Cited by 73 publications

(22 citation statements)

References 63 publications

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“…GSH‐AuNCs were prepared based on previous work with a little modification . Freshly prepared HAuCl 4 (25 mM, 0.4 mL) was mixed with GSH (25 mM, 0.6 mL) and 4 mL ultrapure water under vigorous stirring at ambient temperature, until the solution turned colorless.…”

Section: Methodsmentioning

confidence: 99%

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

Tan

Lan

Yin

et al. 2019

Bulletin Korean Chem Soc

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A magnetic molecularly imprinted polymer-based fluorescent (MIP-FL) probe has been synthesized by embedding glutathione-stabilized gold nanoclusters (GSH-AuNCs) and Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) into silica composites. The Fe 3 O 4 NPs/AuNCs@MIPs are characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and UV-Vis absorption spectroscopy. The results show that the spherical Fe 3 O 4 NPs/AuNCs@MIPs contain a core-shell structure with GSH-AuNCs anchored on the surface of silica-protected Fe 3 O 4 NPs. As a fluorescence probe (excitation: 360 nm, emission: 615 nm), Fe 3 O 4 NPs/AuNCs@MIPs can selectively detect metronidazole (MNZ) among other nitroimidazoles (ronidazole, ornidazole, and tinidazole). During the detection, the fluorescence intensity of the MIP-FL probe drops gradually with increasing MNZ concentration. The sensitive linear range of the fluorescence probe is from 0 to 5 μM, and the limit of detection is 4.2 nM. After the recognition sites interact with the template, the occurrence of charge transfer from the GSH-AuNCs to MNZ results in fluorescence quenching. Finally, a real sample test has been performed in spiked milk. Satisfactory recoveries spanning from 96% to 102% indicate that Fe 3 O 4 NPs/AuNCs@MIPs enable highly sensitive detection of MNZ based on fluorescence signal output, while the MIPs also have enrichment potential for the target from complex samples due to the magnetic properties.

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Section: Methodsmentioning

confidence: 99%

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

Tan

Lan

Yin

et al. 2019

Bulletin Korean Chem Soc

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“…Graphene and graphene oxide (GO) have attracted growing interest in the sensing field in recent years, since aromatic ring structures based on graphene and GO surfaces readily adsorb and combine with many biological molecules (including aptamers) through a π-π stacking mechanism and electron transfer. The resulting aromatic ring structures thus provide an outstanding platform for fluorescence-based sensing [32][33][34][35]. Notably, graphene and its derivatives are beneficial not only in preventing the aptamers from changing their configuration before binding to the target, but also serve as excellent quenchers with minimal background interference.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water

Huang

Mai

et al. 2021

Micromachines

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Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor capable of detecting mercury Hg(II) and lead Pb(II) ions simultaneously, using graphene oxide (GO) as a quenching agent and an aptamer solution as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed well with 500 ppm GO solution before injection into one inlet of the microchannel, and the heavy metal sample solution is injected into another inlet. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluorescence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The selectivity of these two ions is also shown to be clear among other mixed heavy metal ions. The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 0.70 ppb and 0.53 ppb for Hg(II) and Pb(II), respectively, which is lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Organization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.

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“…Graphene and graphene oxide (GO) have attracted growing interest in the sensing field in recent years since aromatic ring structures based on graphene and GO surfaces readily adsorb and combine with many biological molecules (including aptamers) through a π-π stacking mechanism and electron transfer. The resulting aromatic ring structures thus provide an outstanding platform for fluorescence-based sensing [12][13][14][15]. Notably, graphene and its derivatives are beneficial not only in preventing the aptamers from changing their configuration before binding to the target, but also serve as excellent quenchers with minimal background interference.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, while laser engraving has a greatly reduced cost, it induces a high surface roughness and therefore increases the risk of sample blockage in the channels [30]. Similarly, using paper-based devices as a detection platform can also effectively reduce costs and production complexity, but the fibers in the paper may block the sample circulation [15]. Accordingly, the present study employs micro-machining technology (a form of CNC machining process) to fabricate a microfluidic platform for Hg(II) and lead Pb(II) ion detection due to its advantages of speed, a low cost, a simple operation interface, and the ability to manufacture complex structures [36].…”

Section: Introductionmentioning

confidence: 99%

“…1. During the subsequent mixing process, the aptamers bond with the Hg 2+ or Pb 2+ ions to form thymine (T) or guanine(G) complexes, respectively, and the concentration of the ions is detected by observing the corresponding FRET-induced change in the fluorescence intensity [12][13][14][15]37]. Finally, the detection results are compared with those obtained from a commercial instrument -inductively coupled plasma mass spectrometry (ICP-MS).…”

Section: Introductionmentioning

confidence: 99%

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A Microfluidic Aptamer-Based Sensor for Detection of Mercury (II) and Lead (II) Ions in Water

Huang¹,

Wu²,

Yeh³

et al. 2021

Preprint

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Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor for the detection of mercury Hg(II) and lead Pb(II) ions using graphene oxide (GO) as a quenching agent and aptamer solu-tion as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed with 500 ppm GO solution in a microfluidic device. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluores-cence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 2 ppb for both metals, which is signifi-cantly lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Or-ganization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.

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Facile preparation of fluorescent Au nanoclusters-based test papers for recyclable detection of Hg2+ and Pb2+

Cited by 73 publications

References 63 publications

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water

A Microfluidic Aptamer-Based Sensor for Detection of Mercury (II) and Lead (II) Ions in Water

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