Facile Preparation of Core–Shell Magnetic Metal–Organic Framework Nanoparticles for the Selective Capture of Phosphopeptides

Abstract

In regard to the phosphoproteome, highly specific and efficient capture of heteroideous kinds of phosphopeptides from intricate biological sample attaches great significance to comprehensive and in-depth phosphorylated proteomics research. However, until now, it has been a challenge. In this study, a new-fashioned porous immobilized metal ion affinity chromatography (IMAC) material was designed and fabricated to promote the selectivity and detection limit for phosphopeptides by covering a metal-organic frameworks (MOFs) shell onto Fe3O4 nanoparticles, taking advantage of layer-by-layer method (the synthesized nanoparticle denoted as Fe3O4@MIL-100 (Fe)). The thick layer renders the nanoparticles with perfect hydrophilic character, super large surface area, large immobilization of the Fe(3+) ions and the special porous structure. Specifically, the as-synthesized MOF-decorated magnetic nanoparticles own an ultra large surface area which is up to 168.66 m(2) g(-1) as well as two appropriate pore sizes of 1.93 and 3.91 nm with a narrow grain-size distribution and rapid separation under the magnetic circumstance. The unique features vested the synthesized nanoparticles an excellent ability for phosphopeptides enrichment with high selectivity for β-casein (molar ratio of β-casein/BSA, 1:500), large enrichment capacity (60 mg g(-1)), low detection limit (0.5 fmol), excellent phosphopeptides recovery (above 84.47%), fine size-exclusion of high molecular weight proteins, good reusability, and desirable batch-to-batch repeatability. Furthermore, encouraged by the experimental results, we successfully performed the as-prepared porous IMAC nanoparticle in the specific capture of phosphopeptides from the human serum (both the healthy and unhealthy) and nonfat milk, which proves itself to be a good candidate for the enrichment and detection of the low-abundant phosphopeptides from complicated biological samples.

Magnetite nanoparticles surface-modified with a zinc(II)-carboxylate Schiff base ligand as a sorbent for solid-phase extraction of organochlorine pesticides from seawater

“…1a, the peak at 1689 and 1595 cm −1 can be assigned to the C = O stretching vibration and C = N stretching vibration of imino groups [26]. The spectrum of Fe 3 O 4 @MAA demonstrate absorption peaks at, 1595, 1220 and 1416 cm −1 which are correspond to C = O and C-O stretching vibration in carboxyl groups (−COOH) and CH 2 bending vibration, respectively [27]. Fe-O stretching vibration peaks at 571 and 585 cm −1 were observed in the (b) and (c) curves, respectively, which proved the presence of Fe 3 O 4 nanoparticles [28].…”

Fe3O4@MOF Magnetic Nanocomposites: Synthesis and Applications

“…One year later, Chen and co‐workers used the Fe 3 O 4 @MIL‐100(Fe) composite, which was synthesized through the layer‐by‐layer approach, to extract phosphopeptides from human serum . Phosphoproteome has attracted a lot of attention because of the widespread use as a modification for proteins.…”

Nanomaterials in Proteomics

“…In addition to this, they also exhibited sensitive capture ability to phosphopeptides. Moreover, Chen et al coated Fe‐MOF of MIL‐100 onto the surface of magnetic nanoparticles by layer‐by‐layer assembly, which was denoted as Fe 3 O 4 @MIL‐100(Fe) . The synthetic route was displayed in Figure F.…”