Facile potentiometric sensing of gallic acid in edible plants based on molecularly imprinted polymer

Abstract

Molecularly imprinted polymers (MIPs) have become a valuable material in the field of electrochemical sensors, due to their selective recognition capabilities towards target molecules. A low‐cost potentiometric sensor based on molecular imprinting was developed for the measurement of gallic acid (GA) in edible plants. The imprinted polymer was synthesized by bulk polymerization in the presence of trimethylolpropane triacrylate as the cross‐linker and 2,2’‐azo‐bisisobutyronitrile as the initiator. The sensing component of the sensor was fabricated by the incorporation of MIPs in a polyvinyl chloride matrix. The species and amount of MIPs were optimized, and the imprinted poly(methacrylic acid) sensor was examined and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and potential response. The proposed sensor exhibited a fast near‐Nernst response to GA in the range of 1 × 10−5 to 3.2 × 10−4mol/L. The potentiometric measurement of GA in edible plants was checked by high‐performance liquid chromatography, and the two test results showed no significant difference (P > 0.05). The imprinted sensor is applicable to the electrochemical determination of GA in edible plants. Practical Application The proposed MIP‐based potentiometric sensor provided a low‐cost, efficient, and green tool for the rapid determination of the bioactive ingredient GA in edible plants. The knowledge obtained will offer useful reference to the quality control and bioactive assessment of botanical food.

Synthesis of molecularly imprinted polymer based on cooperative imprinting for enrichment of gallic acid in Puer tea

“…Simultaneously, the stretching vibration of CC observed at 1541 cm −1 was due to the benzene skeleton of AAPBA. The similar peaks of the above functional groups have been reported in previous references 34, 43, 44 . Besides, several characteristic peaks assigned to the CH stretching vibration of methyl and methylene groups (about 2985, 2950, 1455 cm −1 ), carbonyl stretching (1725 cm −1 ), CO stretching (1153 cm −1 ) were observed in all polymer spectra, indicating the successful incorporation of EDMA.…”

Biomaterials Technology for AgroFood Resilience

“…[370] In plants, Yang and co-workers utilized a MIP synthesized by bulk polymerization to determine gallic acid, a bioactive food ingredient with antioxidant and antimicrobial effects. [371] In this study, they prepared sensor membranes by combining MIPs and conventional ion-selective electrodes for the potentiometric measurement of gallic acid. Stilman et al prepared ultrathin surface imprinted polymer layers as receptors for impedance spectroscopy and detected yeast (Saccharomyces) strains as low as 30 cells mL −1 from yogurt and beer samples.…”

“…[ 370 ] In plants, Yang and co‐workers utilized a MIP synthesized by bulk polymerization to determine gallic acid, a bioactive food ingredient with antioxidant and antimicrobial effects. [ 371 ] In this study, they prepared sensor membranes by combining MIPs and conventional ion‐selective electrodes for the potentiometric measurement of gallic acid. Stilman et al.…”

New phases for analytical scale extraction from plants: Current and future trends

“…antibiotics, pollutants and toxins) from food or environmental samples is one of the most highly developed applications of MIPs in analytical chemistry [77][78][79]. The use of MIPs in plant analyses follows a different trend, that is the extraction of endogenous bioactive compounds, including molecules with widely differing chemical structures, thanks to the possibility of obtaining tailor-made MIPs [80][81][82][83][84][85][86].…”

“…[105], with permission from Wiley D) potentiometric analysis using MIP-based electrochemical sensor, adapted from Ref. [85], with permission from Institute of Food Technologists; and E) MSPD microextraction using microcrystalline cellulose as sorbent, adapted from Ref. [62], with permission from Elsevier.…”