Facile photosynthesis of novel porphyrin-derived nanocomposites containing Ag, Ag/Au, and Ag/Cu for photobactericidal study

Abstract

In this research, the one-step synthesis of novel porphyrin-based nanocomposites was performed easily using a photochemical under visible light illumination strategy. As a result, the focus of this research is on synthesizing and using decorated ZnTPP (zinc(II)tetrakis(4-phenyl)porphyrin) nanoparticles with Ag, Ag/AgCl/Cu, and Au/Ag/AgCl nanostructures as antibacterial agents. Initially, ZnTPP NPs were synthesized as a result of the self-assembly of ZnTPP. In the next step, in a visible-light irradiation photochemically process, the self-assembled ZnTPP nanoparticles were used to make ZnTPP/Ag NCs, ZnTPP/Ag/AgCl/Cu NCs, and ZnTPP/Au/Ag/AgCl NCs. A study on the antibacterial activity of nanocomposites was carried out for Escherichia coli, and Staphylococcus aureus as pathogen microorganisms by the plate count method, well diffusion tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) values determination. Thereafter, the reactive oxygen species (ROS) were determined by the flow cytometry method. All the antibacterial tests and the flow cytometry ROS measurements were carried out under LED light and in dark. The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was applied to investigate the cytotoxicity of the ZnTPP/Ag/AgCl/Cu NCs, against Human foreskin fibroblast (HFF-1) normal cells. Due to the specific properties such as admissible photosensitizing properties of porphyrin, mild reaction conditions, high antibacterial properties in the presence of LED light, crystal structure, and green synthesis, these nanocomposites were recognized as kinds of antibacterial materials that are activated in visible light, got the potential for use in a broad range of medical applications, photodynamic therapy, and water treatment.

Recyclable and Stable Porphyrin‐Based Self‐Assemblies by Electrostatic Force for Efficient Photocatalytic Organic Transformation

“…The presence of four characteristic absorption peaks within the TPPS Q‐band confirms its metal‐free characteristics (Figure 3a ; Figure S9 , Supporting Information). [ 21 ] Power X‐ray diffraction (XRD) analysis indicates that the distinct peaks observed in the original well‐ordered packing of BV and EDA have disappeared upon assembly into TPPS‐BV and TPPS‐EDA, suggesting that TPPS‐BV and TPPS‐EDA possess amorphous structures (Figure 2d ).…”

Photocatalytic activity of co‐doped NPs based on ZnO as a new class of anti‐microbial agents: Synthesis, characterization, kinetics, isotherm, and in silico molecular docking simulation

“…These materials have a variety of applications, 7 including reduction of CO 2 , degradation of organic contaminants, removal of toxic ions and heavy metal ions, water or air purification, selfcleaning, and antibacterial properties. 8,9 ZnO has many features to become an environmentally friendly metal oxide and properties, such as inexpensive, safe, low toxicity, biodegradable, and easily prepared. 10 Moreover, zinc oxide (ZnO) has emerged as an effective photocatalyst because of its higher H 2 O 2 generation compared with other metal oxide nanomaterials.…”