Facile metabolic glycan labeling strategy for exosome tracking

Lee, Yong Jin; Kim, Young; Zhang, Weiqi; Song, In Ho; Tung, Ching–Hsuan

doi:10.1016/j.bbagen.2018.02.001

Cited by 72 publications

(55 citation statements)

References 43 publications

(47 reference statements)

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“…Especially, genetically labeled exosomes can be utilized to monitor the effects of surface modifications, such as covalent binding or membrane integration, which may cause structural or functional changes in the membranes of exosomes [67]. [74,76] Labeling of exosomes by encapsulation has been performed with various labeling probes such as radioisotopes, nanoparticles, and fluorescent dyes ( Table 4). Passive loading of probes is frequently used.…”

Section: Labeling Methodsmentioning

confidence: 99%

“…To avoid this, methods based on the gel filtration (GF) principle are possible alternatives to conventional SEC. Commercial GF columns are already available to remove free probes by simple centrifugation without a significant increase in the sample volume [44,74,82,97]. Precipitation methods were also used to remove free probes.…”

Section: Exosome Isolation Methodsmentioning

confidence: 99%

“…Metabolic labeling of exosomes is achievable with the addition of specific substances during the cell culture process. After the isolation of metabolically labeled exosomes, covalent binding of the probes can be achieved with click chemistry [74]. However, the addition of extra substances during cell culture may cause changes in the characteristics of the cells or exosomes.…”

Section: Metabolic Labelingmentioning

confidence: 99%

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Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Yi¹,

Lee²,

Kim

et al. 2020

IJMS

141

131

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Exosomes are nano-sized membranous vesicles produced by nearly all types of cells. Since exosome-like vesicles are produced in an evolutionarily conserved manner for information and function transfer from the originating cells to recipient cells, an increasing number of studies have focused on their application as therapeutic agents, drug delivery vehicles, and diagnostic targets. Analysis of the in vivo distribution of exosomes is a prerequisite for the development of exosome-based therapeutics and drug delivery vehicles with accurate prediction of therapeutic dose and potential side effects. Various attempts to evaluate the biodistribution of exosomes obtained from different sources have been reported. In this review, we examined the current trends and the advantages and disadvantages of the methods used to determine the biodistribution of exosomes by molecular imaging. We also reviewed 29 publications to compare the methods employed to isolate, analyze, and label exosomes as well as to determine the biodistribution of labeled exosomes.

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Section: Labeling Methodsmentioning

confidence: 99%

Section: Exosome Isolation Methodsmentioning

confidence: 99%

Section: Metabolic Labelingmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Yi¹,

Lee²,

Kim

et al. 2020

IJMS

141

131

View full text Add to dashboard Cite

show abstract

“…Tetraacetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) is placed in culture with the parent cells, spontaneously incorporated into glycans and uniformly redistributed on their EVs. The azido-EVs are then labeled with azadibenzylcyclooctyne (ADIBO)-fluorescent dyes by a bioorthogonal click reaction [133]. Exploiting the principle of bioluminescence for tracking EVs, in vivo Gaussian Luciferase (Gluc) is linked to a transmembrane domain of a platelet-derived growth factor receptor [134,135], or a lactadherin [136,137].…”

Section: Indirect Surface Functionalizationmentioning

confidence: 99%

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

Susa

Limongi

Dumontel

et al. 2019

Cancers

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Fast diagnosis and more efficient therapies for cancer surely represent one of the huge tasks for the worldwide researchers’ and clinicians’ community. In the last two decades, our understanding of the biology and molecular pathology of cancer mechanisms, coupled with the continuous development of the material science and technological compounds, have successfully improved nanomedicine applications in oncology. This review argues on nanomedicine application of engineered extracellular vesicles (EVs) in oncology. All the most innovative processes of EVs engineering are discussed together with the related degree of applicability for each one of them in cancer nanomedicines.

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“…in vivo tracking of fluorescent labeled cancer-derived exosomes were evaluated in various cells (MCF7 and MDA-MB-231 cells) and tumor bearing mice. Highly metastatic cancer-derived exosomes homed to the tumor and showed selective organ distribution in vivo (mostly in the liver and intestines) (Lee et al, 2018). Taken together, although FLI has drawbacks such as low penetration depth, labeling exosomes with fluorescent indicators provides information not merely about biodistribution and migration abilities of the exosomes, but also on the biological mechanisms that occur in the cell and involve exosomes biogenesis, secretion and communication with high resolution.…”

Section: Optical Imagingmentioning

confidence: 99%

Advances in imaging strategies for in vivo tracking of exosomes

Betzer

Barnoy

Sadan

et al. 2019

WIREs Nanomed Nanobiotechnol

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Exosomes have many biological functions as short‐ and long distance nanocarriers for cell‐to‐cell communication. They allow the exchange of complex information between cells, and thereby modulate various processes such as homeostasis, immune response and angiogenesis, in both physiological and pathological conditions. In addition, due to their unique abilities of migration, targeting, and selective internalization into specific cells, they are promising delivery vectors. As such, they provide a potentially new field in diagnostics and treatment, and may serve as an alternative to cell‐based therapeutic approaches. However, a major drawback for translating exosome treatment to the clinic is that current understanding of these endogenous vesicles is insufficient, especially in regards to their in vivo behavior. Tracking exosomes in vivo can provide important knowledge regarding their biodistribution, migration abilities, toxicity, biological role, communication capabilities, and mechanism of action. Therefore, the development of efficient, sensitive and biocompatible exosome labeling and imaging techniques is highly desired. Recent studies have developed different methods for exosome labeling and imaging, which have allowed for in vivo investigation of their bio‐distribution, physiological functions, migration, and targeting mechanisms. These improved imaging capabilities are expected to greatly advance exosome‐based nanomedicine applications. This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Diagnostic Tools > In Vivo Nanodiagnostics and Imaging Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Facile metabolic glycan labeling strategy for exosome tracking

Abstract: A facile and effective exosome labeling strategy was introduced by presenting azido moiety on the surface of exosome through metabolic glycan synthesis, and then conjugating a strain-promoted fluorescent dye.

Cited by 72 publications

References 43 publications

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

Advances in imaging strategies for in vivo tracking of exosomes

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