Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

Cloete, William J.; Adriaanse, Craig; Klumperman, Bert

doi:10.1039/c1py00069a

Cited by 15 publications

(11 citation statements)

References 13 publications

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“…Our research group has developed a facile approach to enzyme immobilization on electrospun nanofibers containing MAnh residues. This approach does not require rigorous modification or activation steps prior to immobilization, and therefore may provide a low-cost alternative for industrial applications [24]. Here, we extend the previous work.…”

Section: Introductionsupporting

confidence: 53%

“…The synthesis of poly(styrene- alt -maleic anhydride) and subsequent electrospinning into nanofibres were performed as previously described by Cloete et al [24] Briefly, the polymer was produced by copolymerizing, in solution, a 1:1 molar ratio of styrene and maleic anhydride under inert conditions using AIBN as an initiator at 60 °C. Electrospinning of the polymer was done from a 1:2 DMF/acetone solution (15 wt%) with the collector at a distance of 15 cm and offset of 4 cm from the needle tip that was connected to a high voltage supply (25 kV, 400 μA, 10 W) set at 15 kV, and the polymer solution was fed at 0.01 mL·min −1 by means of a feed pump (Harvard, Model 33 Twin Syringe Pump, Holliston, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

2019

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Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone.

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Section: Introductionsupporting

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

2019

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“…As a result of this higher specic surface area, these bers have been used in a wide range of biomedical applications, including drug delivery, [5][6][7][8][9][10] biosensors, 11,12 wound dressings, 13 tissue engineering, 14,15 substrate or skeleton for the growth of nanoparticles, 16,17 and enzyme immobilization. 18 In the pharmaceutical eld, electrospun nanobers have attracted an increasing interest due to their unique structure features. They can be used as carriers for various drugs including antibiotics 19 and anticancer 20 for specic treatment.…”

Section: Introductionmentioning

confidence: 99%

Antibacterials loaded electrospun composite nanofibers: release profile and sustained antibacterial efficacy

et al. 2014

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Synthetic polyacrylonitrile (PAN)/agar composite nanofibers were fabricated by introducing agar during the electrospinning process, and it was found that the nanofibers could be used for efficient, controlled drug release. Hydrophilic drugs such as ampicillin (AMC) were successfully encapsulated inside the agar, allowing the formation of uniform and smooth AMC/agar/PAN composite nanofibers. Cell viability assays showed that the as-prepared agar/PAN composite nanofibers had a good biocompatibility, and the antibacterial activity of the designed drug delivery system was investigated against Gram negative E. coli with a sustained release profile. The good biocompatibility and enhanced thermal properties-as well as the long-lasting antibacterial activity-of these agar/PAN composite nanofiber-containing drugs indicate their significant promise for a variety of potential medicinal applications. We believe that this approach could serve as a model technique in the fields of drug delivery and controlled release when considering the compatibility between polymers and drugs.

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“…Color formation was found to be different depending on both concentration of oxidized ABTS and affinity of the reaction products (obtained from the ABTS enzymatic oxidation) for different textile or polymers and green, blue and purple colors have been reported. 46,47 In our case, different behaviors were obtained based on the enzyme ratio and analyte amount introduced to the system. It could be seen that purple color started to appear when the lactate level reached 5 mM and became dominant until 10 mM.…”

Section: Resultsmentioning

confidence: 92%

Microfluidic device on a nonwoven fabric: A potential biosensor for lactate detection

Baysal

Onder

Göcek

et al. 2014

Textile Research Journal

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In the present study, a novel, wearable textile based microfluidic device was developed that provides a non-invasive, rapid, semi-quantitative detection of the lactate level in simulated sweat solution. The potential application was envisioned to be a biosensor that can monitor an athlete’s physical status during exercise. A photolithography technique was used for the fabrication of hydrophilic micro channels and reservoirs surrounded by hydrophobic barriers made from SU-8 negative photoresist. The reservoirs were functionalized by co-immobilization of lactate oxidase (LOX) and horseradish peroxidase (POX) enzymes. LOX uses L-(+)-Lactic acid as substrate and produces H2O2 which is a POX substrate. Then, POX oxidases H2O2 in the presence of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) and results in color formation. The studies showed that excess amount of analyte presence resulted in analyte inhibition. It was also shown that analyte pH and temperature were effective on the color formation. For effective results, analyte pH and temperature should be ≥5℃ and 25–30℃, respectively. Lower pH and higher temperature values resulted in a decrease in the enzyme activity. The textile based biosensor system could make a semi-quantitative visual detection to differentiate between the normal (<5 mM) and high (≥5 mM) lactate level: while a high lactate level led to a denser purple color formation, normal levels led to a light purple formation and a green color started to be observed.

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Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

Cited by 15 publications

References 13 publications

Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

Antibacterials loaded electrospun composite nanofibers: release profile and sustained antibacterial efficacy

Microfluidic device on a nonwoven fabric: A potential biosensor for lactate detection

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