Facile green synthesis of baicalein fabricated gold nanoparticles and their antibiofilm activity against Pseudomonas aeruginosa PAO1

Rajkumari, Jobina; Siddhardha, Busi; Vasu, Aneesh Cherukunnel; Reddy, P Ravi Tejeshwar

doi:10.1016/j.micpath.2017.03.044

Cited by 70 publications

(32 citation statements)

References 22 publications

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“…Similar results of biofilm inhibition using CLSM were shown by Subhaswaraj et al [43]. Our findings were also phenotypically similar to those of Rajkumari et al [36]. They showed the decrease in the biofilm-forming ability of PAO1 using Baicalin-conjugated nanoparticles.…”

Section: Discussionsupporting

confidence: 91%

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Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

et al. 2020

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Plant-based synthesis of eco-friendly nanoparticles has widespread applications in many fields, including medicine. Biofilm—a shield for pathogenic microorganisms—once formed, is difficult to destroy with antibiotics, making the pathogen resistant. Here, we synthesized gold nanoparticles (AuNPs) using the stem of an Ayurvedic medicinal plant, Tinospora cordifolia, and studied the action of AuNPs against Pseudomonas aeruginosa PAO1 biofilm. The synthesized AuNPs were characterized by techniques such as ultraviolet-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray diffraction, X-ray diffraction, scanning electron microscopy (SEM), and transmission electron microscopy. The AuNPs were spherically shaped with an average size of 16.1 nm. Further, the subminimum inhibitory concentrations (MICs) of AuNPs (50, 100, and 150 µg/mL) greatly affected the biofilm-forming ability of P. aeruginosa, as observed by crystal violet assay and SEM, which showed a decrease in the number of biofilm-forming cells with increasing AuNP concentration. This was further justified by confocal laser scanning microscopy (CLSM), which showed irregularities in the structure of the biofilm at the sub-MIC of AuNPs. Further, the interaction of AuNPs with PAO1 at the highest sub-MIC (150 µg/mL) showed the internalization of the nanoparticles, probably affecting the tendency of PAO1 to colonize on the surface of the nanoparticles. This study suggests that green-synthesized AuNPs can be used as effective nano-antibiotics against biofilm-related infections caused by P. aeruginosa.

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Section: Discussionsupporting

confidence: 91%

“…This was due to the surface plasmon vibrations with aqueous AuNPs [35]. Rajkumari et al [36] obtained similar results by obtaining the UV spectra of green-synthesized AuNPs at 550 nm.…”

Section: Discussionmentioning

confidence: 66%

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

et al. 2020

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“…The loss of biofilm formation and swimming motility ability of RS-2 when treated with MgO and MnO 2 nanoparticles indicates less virulence when invading and colonizing host tissue. The suppression of biofilm formation and swimming motility observed is consistent with the studies of [33,48] who reported a concentration-dependence on the ability to suppress biofilm formation and the motility of the bacteria.…”

Section: Antibacterial Activity Of Mgo and Mno 2 Nanoparticlessupporting

confidence: 91%

“…Biofilm formation has been attributed to contributing to the antibiotic resistance in bacterial [48]. The loss of biofilm formation and swimming motility ability of RS-2 when treated with MgO and MnO 2 nanoparticles indicates less virulence when invading and colonizing host tissue.…”

Section: Antibacterial Activity Of Mgo and Mno 2 Nanoparticlesmentioning

confidence: 99%

Biosynthesis and characterization of magnesium oxide and manganese dioxide nanoparticles using Matricaria chamomilla L. extract and its inhibitory effect on Acidovorax oryzae strain RS-2

Ogunyemi

Zhang

Abdallah

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

114

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“…To determine the efficacy of AgNPs in inhibiting established biofilm, each well of sterile microtiter plate was filled with 100 μL overnight grown bacterial suspensions and the plates were incubated for 24 hours. After incubation, the established biofilms in the plates were then added with 100 μL of different concentrations of AgNPs (i.e 0.1, 0.5, 1, 5, 10, 25, and 50 μg/mL) and incubated for another 24 hours at 37 o C. Similarly, biofilm mass was evaluated using crystal violet staining (Barapatre et al, 2006;Kanmani et al, 2013;Rajkumari et al, 2017;Cho et al, 2013). For quantification of the total biofilm formation, the absorbance was recorded at a wavelength of 570 nm.…”

Section: Anti-biofilm Activity Of the Silver Nanoparticlesmentioning

confidence: 99%

Bacterial Exopolysaccharide-Mediated Synthesis of Silver Nanoparticles and Their Application on Bacterial Biofilms

Galvez¹,

Ramos²,

Teja³

et al. 2019

J microb biotech food sci

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The development of multi-drug-resistant bacteria and their biofilm formation capabilities has decreased the effectiveness of antibiotics and other antimicrobial agents. Silver nanoparticle (AgNP) is currently being explored as an alternative strategy in countering biofilm-related infections. In this study, AgNP was biosynthesized using the extracted exopolysaccharide (EPS) from two obligate alkaliphilic bacteria, Bacillus krulwichiae M2.5 and Bacillus cellulosilyticus M4.1, isolated from a hyperalkaline spring in Zambales, Philippines. The samples exhibited an absorption peak at around 420 nm corresponding to the surface plasmon resonance of AgNPs as shown by UV-Vis analysis. The Fourier transform infrared (FTIR) spectroscopy spectra revealed functional groups in the EPS such as hydroxyl and carboxyl involved in the reduction of Ag+ to AgNP. Scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) spectroscopy showed stable irregular and spherical AgNPs with an average size of 25.88 ± 10.49 nm and 23.99± 8.43 nm for B. krulwichiae M2.5 EPS-AgNP and B. cellulosilyticus M4.1 EPS-AgNP, respectively. The biosynthesized AgNPs significantly inhibited the biofilm formation of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus in a dose-dependent manner as determined by microtiter plate assay. However, the results showed a reduced inhibitory effect of the synthesized AgNPs on established biofilms indicating the need for higher AgNP concentration. This study demonstrates that EPS from bacteria adapted to alkaline conditions can be used for efficient AgNP biosynthesis with potential biomedical applications.

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Facile green synthesis of baicalein fabricated gold nanoparticles and their antibiofilm activity against Pseudomonas aeruginosa PAO1

Cited by 70 publications

References 22 publications

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

Biosynthesis and characterization of magnesium oxide and manganese dioxide nanoparticles using Matricaria chamomilla L. extract and its inhibitory effect on Acidovorax oryzae strain RS-2

Bacterial Exopolysaccharide-Mediated Synthesis of Silver Nanoparticles and Their Application on Bacterial Biofilms

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