Facile extraction of chitin and chitosan from shrimp shell

Hisham, Firzanah; Hatta, Maziati Akmal Mohd; Ahmad, Fawwad; Ahmad, Khaliq

doi:10.1016/j.matpr.2020.12.329

Cited by 22 publications

(13 citation statements)

References 15 publications

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“…The two critical steps were the deproteinization and demineralization. In this study, the removal of protein was carried out using sodium hydroxide prior to the demineralization to control the formation of foam during the treatment process and hence, could optimize the yield of chitin [ 49 ]. The demineralization step was successfully accomplished by treating the shrimp waste with 1.0 mol L − 1 hydrochloric acid solution to dissolve calcium carbonate and remove other organic matters.…”

Section: Resultsmentioning

confidence: 99%

New Isolated Shrimp (Litopenaeus setiferus) Chitosan-Based Films Loaded with Fly Ash for Antibacterial Evaluation

Alterary

Marei

2022

Polymers

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New three fabricated chitosan (CS) loaded with fly ash (FA) films were developed in this study. The shell waste of white shrimp was used as a precursor for the isolation of chitin and converted into chitosan by carrying out a deacetylation process. The formation of chitosan was conducted by various preparation steps deproteinization, demineralization, and deacetylation. The degree of deacetylation was found to be 95.2%. The obtained chitosan was used to prepare three different chitosan loaded-fly ash films. The prepared films contained various fly ash: chitosan ratios (2:1, FA-CSF1), (1:1, FA-CSF2), and (1:2, FA-CSF3). The obtained films were characterized using FTIR, XRD, and SEM. The micrograph images of the formed films showed spherical particles with an average size of 10 µm. The surface area, adsorption-desorption properties, thermal stability, and water/fat binding features of the fabricated chitosan films were studied. The results revealed that the prepared films displayed typical BET graphs with surface areas ranging from 2.436 m2 g−1 to 8.490 m2 g−1. The fabricated FA-CSF films also showed high thermal stability at temperatures up to 284.9 °C and excellent water/fat binding capacities. The antibacterial potential of the designed films was screened against E. coli (Gram-negative) and B. cereus (Gram-positive) bacterial strains. The tested solution of CS (1%) exhibited inhibition zones for E. coli and B. cereus as 18.51 mm and 14.81 mm, respectively, while in FA solution (1%), the inhibition zones were found to be 10.16 mm, and 13.57 mm, respectively. The results encourage and open up the new and promising areas of research for applying chitosan extracted from waste materials in biological applications.

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Section: Resultsmentioning

confidence: 99%

New Isolated Shrimp (Litopenaeus setiferus) Chitosan-Based Films Loaded with Fly Ash for Antibacterial Evaluation

Alterary

Marei

2022

Polymers

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“…Deproteinization started with 60 grams of shrimp shell powder dissolved in NaOH 1 M solution with the ratio of powder to solvent was 1 : 5 (w/v) for 20 hours at 28±2 °C. The mixture was filtered, the shrimp powder residue washed until pH 7, dried for 30 minutes at 60 °C and weighted [34] . The deproteinated powder was then demineralized using HCl 1 M solution with the ratio of powder to solvent at 1 : 5 (w/v) for 16 hours at 28±2 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The deproteinated powder was then demineralized using HCl 1 M solution with the ratio of powder to solvent at 1 : 5 (w/v) for 16 hours at 28±2 °C. The mixture was filtered, the shrimp powder residue was washed until pH 7, dried for 30 minutes at 60 °C, and chitin was weighed [34] . The residue of chitin was dissolved in NaOH 50 % (w/v) with the ratio of powder to solvent at 1 : 10 (w/v) for 20 hours at a temperature of 60 °C to remove the acetyl group or deacetylation and produce chitosan.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Hydroxyapatite Composite from Cuttlebone (Sepia sp.) with Chitosan via In Situ as Antibacterial

Jamarun,

Caniago,

Septiani

et al. 2024

ChemistrySelect

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Hydroxyapatite/chitosan (HAp/CTS) was synthesized using the in situ sol‐gel method with pH variation (7, 8, 9, 10, and 11). Based on XRF, cuttlebone, as a source of calcium, contains 96.3 % CaO. The presence of OH− ions from chitosan affects the product mass of composites. FTIR spectra of composites showed the appearance of characteristic vibrations of HAp and chitosan. The cystallite size measured from XRD pattern using Scherrer equation obtained as 20.18 nm and 17.69 nm for HAp and the HAp/CTS‐11 composite, respectively. The morphology of HAp/CTS‐11 was agglomerated spherical with a Ca/P ratio of 1.86 based on SEM‐EDS analysis. Also, the HAp/CTS‐11 composite revealed antibacterial activity against Escherichia coli and Staphylococcus aureus. HAp/CTS‐11 may be a promising biomaterial for bone implants with antibacterial activity to overcome osteomyelitis.

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“…It only partially dissolves in organic acids, although it dissolves in aqueous acids with a pH of less than 6.5 and is insoluble in sulfuric and phosphoric acids. 13,14 Chitosan has proved effective as a carrier system because of its biological characteristics and aqueous solubility. Moreover, it is hypothesized that a combination of mucoadhesion and impact on mucosal cell membrane tight junction regulation is the mechanism of action behind its excellent transdermal penetration enhancement capabilities.…”

Section: Introductionmentioning

confidence: 99%

“…Crab, shrimp, and lobster shells contain the natural polymer chitosan, which is produced when chitin is deacetylated. It only partially dissolves in organic acids, although it dissolves in aqueous acids with a pH of less than 6.5 and is insoluble in sulfuric and phosphoric acids 13,14 . Chitosan has proved effective as a carrier system because of its biological characteristics and aqueous solubility.…”

Section: Introductionmentioning

confidence: 99%

Alkylated chitosan nanomaterials for enhanced antimicrobial activity and delivery of fusidic acid: Preparation and preliminary in vitro investigation

Bostanudin

Salam

Kayed

et al. 2023

J of Applied Polymer Sci

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Nanomaterials manufactured of amphiphilically‐altered polysaccharides have sparked a great deal of interest due to their capability to elevate drug transport across the skin. The study presents the fabrication of alkylated chitosan through 2‐tert‐butoxymethyloxirane alteration (alkylation degree 29.7%), that was typified by spectroscopic, chromatographic, and thermal analysis approaches before being constructed into nanomaterials for the analysis of their capability in governing fusidic acid diffusion percutaneously. The nanomaterials were constructed via the ionic interaction of positively charged chitosan and negatively charged cross linker sodium tripolyphosphate, with a loading degree of fusidic acid of ca. 20% reported. Fusidic acid release was slower in nanomaterials constructed of alkylated chitosan than in neat chitosan. Under application‐relevant conditions, in vitro incubation with HaCaT cells exhibited insignificant toxicity. Using Franz diffusion cells, the alkylated chitosan nanomaterials induced fusidic acid to infiltrate the Strat‐M® membrane at a 2‐fold greater rate than the neat chitosan nanomaterials. Based on the agar diffusion test, fusidic acid was shown to have better antimicrobial activity when loaded into alkylated chitosan nanomaterials than when loaded into neat chitosan nanomaterials. Ultimately, the in vitro results revealed that alkylated chitosan nanomaterials offer a great deal of potential for percutaneous delivery, which merits greater analysis.

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Facile extraction of chitin and chitosan from shrimp shell

Cited by 22 publications

References 15 publications

New Isolated Shrimp (Litopenaeus setiferus) Chitosan-Based Films Loaded with Fly Ash for Antibacterial Evaluation

New Isolated Shrimp (Litopenaeus setiferus) Chitosan-Based Films Loaded with Fly Ash for Antibacterial Evaluation

Synthesis and Characterization of Hydroxyapatite Composite from Cuttlebone (Sepia sp.) with Chitosan via In Situ as Antibacterial

Alkylated chitosan nanomaterials for enhanced antimicrobial activity and delivery of fusidic acid: Preparation and preliminary in vitro investigation

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