Facile construction of a reusable multi-enzyme cascade bioreactor for effective fluorescence discrimination and quantitation of amino acid enantiomers

Signal amplification surface-enhanced Raman scattering immunosorbent assay of human chorionic gonadotrophin based on repeated enzyme biocatalytic precipitation

“…Briefly, HRP catalyzes the oxidation of OPD by H 2 O 2 to produce DAP molecules. 39,40 The SERS spectrum of product DAP is significantly different from that of the substrate OPD. For example, as shown in Fig.…”

Co‐Immobilization of GOD & HRP on Y‐Shaped DNA Scaffold and the Regulation of Inter‐Enzyme Distance

“…[13] Traditional multienzymes coimmobilization methods, including adsorption, covalent binding, and cross-linking, are all random coimmobilization, which is difficult to accurately regulate the spatial position of enzyme and the inter-enzyme distance and thus results in low cascade efficiency. [10,14] To solve this problem and make multiple enzymes to generate substrate channeling effect, [15] various new strategies and artificial scaffolds are developed to immobilize multiple enzymes, [13,16,17,18] such as layer-by-layer coimmobilization strategy to coimmobilize glucose oxidase (GOD) and horseradish peroxidase (HRP), [19] surface displayed fusion technology to establish multienzymes complex, [14] MOF co-encapsulation strategy to co-immobilize GOD and HRP, [20] etc. Recently, Mafferis et al also utilized the nano chamber "cluster" to control the distance between GOD and lactoperoxidase, effectively improving the cascade efficiency.…”

Simultaneous Discrimination and Quantification of Enantiomeric Amino Acids under Physiological Conditions by Chiral 19F NMR Tag

“…An abnormal d -amino acid level is associated with various symptoms, such as schizophrenia and Alzheimer’s disease. − Discrimination and quantification of enantiomeric molecules is highly desirable and is a routine necessity in the field of biological processes, synthetic chemistry, and the pharmaceutical industry. Methods toward chiral analysis, including high-performance liquid chromatography, − mass spectrometry, − circular dichroism, − and fluorescence − were proposed. The optical analysis of amino acids that have low or no UV absorption generally requires chemical derivatization followed by chiral chromatography. , Fluorescence spectroscopy is a sensitive method to determine enantiomeric amino acids via coordination by a chiral host or a covalent attachment to a fluorophore, , however, the varied binding strength between different analytes with the host or the reversible process between the free host and the host–guest complex compromises the quantification of individual analytes in a mixture .…”