Facile Chemoenzymatic Synthesis of O‐Mannosyl Glycans

Abstract

OMannosylation is av ital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lacko fs tructurally defined glycoforms.A ne fficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzedb yg lycosyltransferases.I nt otal, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.S ubsequent glycan microarraya nalysis revealed fine specificities of glycan-binding proteins and specific antisera.