Facile assay method for norfloxacin and ciprofloxacin by sequential injection chromatography

Abstract

Summary. Sequential injection chromatography (SIC) is a simple, inexpensive, portable, reagent-saving, and environmentally benign separation technique. This paper reports the development of a facile assay method for norfloxacin (NF) and ciprofloxacin (CF) in their formulations (tablets and eye drops) using SIC. The analytes were rapidly chromatographed on a short C18 monolithic column (4.6 × 50 mm) at a flow rate of 30 μL s −1 . An isocratic mode was adopted. The optimum mobile phase composition was 90% water, 10% acetonitrile, and 0.3% triethylamine at pH 3.0. Miniaturized fiber optic spectrometric devices were coupled to the SIC system for UV detection at 280 nm. Sufficient separation with a resolution of 1.6 and numbers of theoretical plates of more than 1500 was achieved. Additionally, acceptable peak shapes with peak symmetries of 0.89 and 0.92 for NF and CF, respectively, were obtained. The method was validated and found to be reliable (the recovery was 98.0-100.6%) and precise (the RSDs of intra-/inter-day precision were less than 2.0%). The method is also sensitive. The limits of detection were less than 0.27 μg mL −1 . The provided method could be considered an industrial scale since the sample frequency was seven samples h −1 and the total volume of consumed reagents was 11.5 mL.

Flow Analysis: A Novel Approach For Classification

“…SIA compared to CFA and FIA allows to reduce the reagent consumption and waste generation significantly. In this respect, the most progressive The sequential injection chromatography [49][50][51][52] should be also included to the DCFM.…”

Development of a low pressure chromatographic flow system for monitoring the biodegradation of ofloxacin and ciprofloxacin

“…27,28 This type of column has already been applied to the determination of FQs. 5,29,30 However, the studies by Pena et al and Seifrtová et al 5,29 use an HPLC platform which requires expensive instrumentation, and the work by Elgorashe et al 30 describes a sequential injection low pressure chromatography method that was applied to pharmaceutical samples.…”

Estratégias para aumento de sensibilidade e seletividade de separações cromatográficas em sistemas de análises em fluxo

“…Fase móvel ácida foi utilizada para a eluição pela protonação das sulfonamidas. A eluição deve ser rápida e quantitativa, afim de se obter uma zona estreita de analitos para introdução na coluna cromatográfica para separação, A resina com grupos funcionais 2-dietilamino-etil (Figura 4.9) apresentou maior dependência do pH da amostra do que aquela com grupos aminopropil, pois apresenta maior valor de pka (11,5), permanecendo predominantemente protonada em toda a faixa de pH avaliada. O aumento do pH resultou em maior eficiência de extração e pré-concentração para a maioria das espécies devido à maior ionização A Figura 4.15 apresenta uma comparação das resinas avaliadas em condições de pH de amostra e fase móvel ótimas para cada uma delas, evidenciando o melhor desempenho da resina 3-trimetilamino-2-hidroxipropil, a qual apresentou melhor desempenho com amostra em pH 11,0 e fase móvel com pH 5,0.…”