Facile and rapid generation of 3D chemical gradients within hydrogels for high-throughput drug screening applications

Ahadian, Samad; Ramón‐Azcón, Javier; Estili, Mehdi; Obregón, Raquel; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1016/j.bios.2014.03.031

Cited by 36 publications

(30 citation statements)

References 41 publications

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“…The addition of gold [77, 78] and other inorganic particles [79, 80] has been used to improve the mechanical strengths [81], conductivity [77], response to thermal or magnetic stimuli, and biological functionality of GelMA hydrogels [78]. For example, Heo et al .…”

Section: Hybrid Hydrogels Based On Gelmamentioning

confidence: 99%

“…For example, dielectrophoresis (DEP) techniques can be used to engineer accurate 3D concentration gradients of micro and nanoparticles loaded with drugs (or other compounds) in GelMA hydrogels (Figure 6A) [77]. The nanoparticle concentration gradient and the degree of crosslinking of the hydrogels offer the possibility of customizing programmed release profiles.…”

Section: Biomedical Applications Of Gelma Hydrogelsmentioning

confidence: 99%

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Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

et al. 2015

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Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.

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Section: Hybrid Hydrogels Based On Gelmamentioning

confidence: 99%

Section: Biomedical Applications Of Gelma Hydrogelsmentioning

confidence: 99%

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

et al. 2015

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“…bacteria [93]. Moreover, by integrating a dielectrophoresis system with gold microparticle-laden GelMA hydrogels, the controlled release of drugs was achieved with a concentration gradient for high-throughput drug screening applications [34]. On the other hand, by incorporating chitosan nanoparticles into GelMA hydrogel, which provides a sustained release of basic fibroblast growth factor, this hybrid hydrogel can be used for angiogenesis in various diseases, particularly ischemic diseases [75].…”

Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

“…For drug delivery, photo-crosslinkable hydrogels offer the opportunity for on-demand delivery of different therapeutics (e.g.,drugs and growth factors) in a single cytocompatible hydrogel-based drug-delivery system with high loading efficiency. The hydrogel could achieve dose-controlled and timed release of drugs at desired sites for therapeutic applications [34]. …”

Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. We first highlight the advantages of photopolymerization and discuss diverse photosensitive systems used for the synthesis of photo-crosslinkable hydrogels. We then introduce their synthesis methods and review their latest state of development in biomedical applications, including tissue engineering and regenerative medicine, drug delivery, cancer therapies and biosensing. Lastly, the existing challenges and future perspectives of engineering photo-crosslinkable hydrogels for biomedical applications are briefly discussed.

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“…stiffness), could be a valuable tool to study stem cell fate in 3D and guide tissue regeneration. Hydrogels containing gradients of micro-and nanoparticle-immobilized drugs have also been generated for high-throughput drug screening applications (Ahadian et al, 2014). Briefly, dielectrophoresis was used to manipulate gold micro-and nano-particles of different sizes within GelMA hydrogels in a rapid and facile way to generate 3D gradients in a micro chamber.…”

Section: Engineering 3d Cellular Gradient Microenvironmentmentioning

confidence: 99%

Hydrogel-based methods for engineering cellular microenvironment with spatiotemporal gradients

Wang

Huang

et al. 2015

Critical Reviews in Biotechnology

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Natural cellular microenvironment consists of spatiotemporal gradients of multiple physical (e.g. extracellular matrix stiffness, porosity and stress/strain) and chemical cues (e.g. morphogens), which play important roles in regulating cell behaviors including spreading, proliferation, migration, differentiation and apoptosis, especially for pathological processes such as tumor formation and progression. Therefore, it is essential to engineer cellular gradient microenvironment incorporating various gradients for the fabrication of normal and pathological tissue models in vitro. In this article, we firstly review the development of engineering cellular physical and chemical gradients with cytocompatible hydrogels in both two-dimension and three-dimension formats. We then present current advances in the application of engineered gradient microenvironments for the fabrication of disease models in vitro. Finally, concluding remarks and future perspectives for engineering cellular gradients are given.

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Facile and rapid generation of 3D chemical gradients within hydrogels for high-throughput drug screening applications

Cited by 36 publications

References 41 publications

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Hydrogel-based methods for engineering cellular microenvironment with spatiotemporal gradients

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