Facile and mild preparation of fluorescent ZnO nanosheets and their bioimaging applications

Jiang, Hui; Wang, Huangping; Wang, Xuemei

doi:10.1016/j.apsusc.2011.03.053

Cited by 30 publications

(12 citation statements)

References 28 publications

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“…The pictures show the localization of quantum dots (observed as fluorescence emission) around and inside cells, which is a proof that QDs were attached onto cell membrane or penetrated into the cells. Our observations are consistent with those described in the work on interactions of fluorescent crystalline ZnO hexagonal nanosheets with K652 cells (Jiang et al 2011), where their applicability for bio-imaging was confirmed. The fluorescence emission was observed in that case around or inside the cells after their culturing with ZnO nanosheets.…”

Section: Resultssupporting

confidence: 92%

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

et al. 2019

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The purpose of the presented work was to modify the surface of core/shell-type CdSe x S 1-x /ZnS quantum dots (QDs) with an anticancer drug to form bifunctional nanoconjugate for imaging and drug delivery. 6-Thioguanine (6-TG) which is used to treat acute myeloid and lymphoblastic leukemia was applied for this aim. The modification of the nanocrystals was carried out using a biphasic method, based on transferring of QDs from the organic phase to the aqueous one with the simultaneous exchange of hydrophobic ligand to the hydrophilic one. To enable a complex evaluation of 6-TG bioactivity, the surface of quantum dots was also modified using mercaptoacetic acid (MAA). Mercaptoacetic acid was chosen as a biologically neutral ligand; so, it was possible to evaluate the effect of nanocrystals toxicity without the participation of the drug (6-TG ligand versus neutral ligand). Moreover, the effect of 6-TG itself on cells was also studied. The studies were carried out on three cell lines: K562 (human myeloid leukemia), A549 (human lung cancer cells) and MRC-5 (normal human lung cells). Evaluation of the cytotoxicity of nanostructures, at various concentrations, was carried out after 24-and 48-h incubation of the cell culture with nanoparticles/drug solutions. MTT and the Alamar Blue assays were used to determine cell viability. Based on the cytotoxicity measurements, it was found that quantum dots modified with 6-TG are more toxic than the drug itself or QDs modified with 3-mercaptoacetic acid. Images taken using fluorescence and confocal microscopy allowed to define the location of examined QDs inside cells.

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Section: Resultssupporting

confidence: 92%

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

et al. 2019

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“…Jiang et al constructed ZnO nanosheets for the imaging of cultured cells. They treated drug sensitive leukemia line K562 cells with ZnO nanosheets, and the yellow-orange light emission was clearly observed around or inside the cells under UV irradiation (365 nm) at room temperature [ 122 ]. ZnO nanostructures were successfully attached onto or penetrated into the cells, which suggested that ZnO nanosheets with visible yellow-orange emission could act as a feasible label for the bioimaging.…”

Section: Biomedical Applications Of Zno Nanoparticlesmentioning

confidence: 99%

The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications

Jiang

Cai

2018

Bioinorganic Chemistry and Applications

880

463

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Zinc oxide nanoparticles (ZnO NPs) are used in an increasing number of industrial products such as rubber, paint, coating, and cosmetics. In the past two decades, ZnO NPs have become one of the most popular metal oxide nanoparticles in biological applications due to their excellent biocompatibility, economic, and low toxicity. ZnO NPs have emerged a promising potential in biomedicine, especially in the fields of anticancer and antibacterial fields, which are involved with their potent ability to trigger excess reactive oxygen species (ROS) production, release zinc ions, and induce cell apoptosis. In addition, zinc is well known to keep the structural integrity of insulin. So, ZnO NPs also have been effectively developed for antidiabetic treatment. Moreover, ZnO NPs show excellent luminescent properties and have turned them into one of the main candidates for bioimaging. Here, we summarize the synthesis and recent advances of ZnO NPs in the biomedical fields, which will be helpful for facilitating their future research progress and focusing on biomedical fields.

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“…Such nanotools indeed could be intrinsically toxic (Bisht and Rayamajhi, 2016), e.g., through the release of metal ions, or could be remotely activated to achieve cell death, as in the photothermal and photodynamic therapies (Lim et al, 2015). Zinc Oxide (ZnO) in particular has raised researchers' interest thanks to its biocompatibility and peculiar piezoelectric and semiconductive properties (Jiang et al, 2018;Racca et al, 2018) useful for its exploitation for imaging (Jiang et al, 2011), biosensing (Sanginario et al, 2016;Shanmugam et al, 2017;Stassi et al, 2017), tissue engineering (Laurenti and Cauda, 2017) and drug delivery (Laurenti and Cauda, 2018;Martínez-Carmona et al, 2018) purposes. Remarkably, ZnO nanoparticles are also studied for their intrinsic anticancer properties thanks to their selective toxicity toward cancer cells (Bisht and Rayamajhi, 2016).…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

Racca

Limongi

Vighetto

et al. 2020

Front. Bioeng. Biotechnol.

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In the last years, different nanotools have been developed to fight cancer cells. They could be administered alone, exploiting their intrinsic toxicity, or remotely activated to achieve cell death. In the latter case, ultrasound (US) has been recently proposed to stimulate some nanomaterials because of the US outstanding property of deep tissue penetration and the possibility of focusing. In this study, for the first time, we report on the highly efficient killing capability of amino-propyl functionalized ZnO nanocrystals (ZnO NCs) in synergy with high-energy ultrasound shock waves (SW) for the treatment of cancer cells. The cytotoxicity and internalization of ZnO NCs were evaluated in cervical adenocarcinoma KB cells, as well as the safety of the SW treatment alone. Then, the remarkably high cytotoxic combination of ZnO NCs and SW was demonstrated, comparing the effect of multiple (3 times/day) SW treatments toward a single one, highlighting that multiple treatments are necessary to achieve efficient cell death. At last, preliminary tests to understand the mechanism of the observed synergistic effect were carried out, correlating the nanomaterial surface chemistry to the specific type of stimulus used. The obtained results can thus pave the way for a novel nanomedicine treatment, based on the synergistic effect of nanocrystals combined with highly intense mechanical pressure waves, offering high efficiency, deep and focused tissue penetration, and a reduction of side effects on healthy cells.

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Facile and mild preparation of fluorescent ZnO nanosheets and their bioimaging applications

Cited by 30 publications

References 28 publications

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

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