Facile and green fabrication of biocatalytic chitosan beads by one-step genipin-mediated β-glucosidase immobilization for production of bioactive genistein

Phadungcharoen, Noppharat; Winotapun, Weerapath; Khomniyawanit, Awattha; Krataichan, Fontip; Rojanarata, Theerasak

doi:10.1016/j.scp.2019.100187

Cited by 9 publications

(5 citation statements)

References 56 publications

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“…It is worth mentioning that other research teams have also used genipin as a matrixactivating compound for immobilisation, and their results confirm our assumptions about its potential use in the immobilisation of cellobiose dehydrogenase. Moreover, there are several literature reports on biomedical applications of genipin [52][53][54].…”

Section: Immobilisation Of Enzymes On Chitosan Microspheresmentioning

confidence: 99%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

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Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications.

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Section: Immobilisation Of Enzymes On Chitosan Microspheresmentioning

confidence: 99%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

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“…Endoglucanase is the main enzyme involved in the hydrolysis of biomass; it initiates cellulose hydrolysis and increases the cellulose hydrolysis rate. Ten genes encoded β-glucosidase, which hydrolyzes cellobiose into glucose [57,58] and is the rate-limiting component of the cellulase-hydrolyzing reaction [59]. The biosynthesis of cellulose can be promoted by sucrose synthase [60], and three genes encoded sucrose synthase.…”

Section: Genes Involved In Photosynthesis and Carbon Metabolismmentioning

confidence: 99%

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Wang

et al. 2021

Plant Physiology and Biochemistry

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BackgroundDrought is one of the main abiotic factors that affect alfalfa yield. The identi cation of genes that control this complex trait can provide important insights for alfalfa breeding. However, little is known about how alfalfa responds and adapts to drought stress, particularly in cultivars of differing drought tolerance. ResultsIn this study, the drought-tolerant cultivar Dryland 'DT' and the drought-sensitive cultivar WL343HQ 'DS' were used to characterize leaf and root physiological responses and transcriptional changes in response to water de cit. Under drought stress, Dryland roots (DTR) showed more differentially expressed genes than WL343HQ roots (DSR), whereas WL343HQ leaves (DSL) showed more differentially expressed genes than Dryland leaves (DTL). Many of these genes were involved in stress-related pathways, carbohydrate metabolism, and lignin and wax biosynthesis, which may have improved the drought tolerance of alfalfa. We also observed that several genes related to ABA metabolism, root elongation, peroxidase activity, cell membrane stability, ubiquitination, and genetic processing responded to drought stress in alfalfa. We highlighted several candidate genes, including sucrose synthase, xylan 1,4-betaxylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA reductase, for future studies on drought stress resistance in alfalfa and other plant species. ConclusionsIn summary, our results reveal the unique drought adaptation and resistance characteristics of two alfalfa genotypes. These ndings, which may be valuable for drought resistance breeding, warrant further gene functional analysis to augment currently available information and to clarify the drought stress regulatory mechanisms of alfalfa and other plants. HighlightsWe assembled leaf and root transcriptomes of drought-tolerant and drought-sensitive alfalfa cultivars under control and drought conditions.Under drought, the drought-tolerant cultivar exhibited more differentially expressed metabolites than the drought-sensitive cultivar.Genes associated with carbon metabolism, amino acid metabolism, hormones, and secondary metabolites were differentially expressed under drought stress.The drought-tolerant cultivar exhibited higher expression of genes encoding sucrose synthase, xylan 1,4-beta-xylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA.

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“…In another paper, commercial β-glucosidase was immobilized by mixing it with chitosan beads and genipin [101]. The biocatalysts beads improved their resistance under acidic conditions after crosslinking.…”

Section: Enzyme Immobilization On Preexisting Supports Using Genipinmentioning

confidence: 99%

“…The genipin-immobilized enzyme was also more stable than the free or the glutaraldehyde-immobilized enzyme. This biocatalyst was applied to hydrolyze genistin to genistein, maintaining 85% of conversion after 5 cycles [101].…”

Section: Enzyme Immobilization On Preexisting Supports Using Genipinmentioning

confidence: 99%

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications

et al. 2019

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Genipin is a reagent isolated from the Gardenia jasminoides fruit extract, and whose low toxicity and good crosslinking properties have converted it into a reactive whose popularity is increasing by the day. These properties have made it widely used in many medical applications, mainly in the production of chitosan materials (crosslinked by this reactive), biological scaffolds for tissue engineering, and nanoparticles of chitosan and nanogels of proteins for controlled drug delivery, the genipin crosslinking being a key point to strengthen the stability of these materials. This review is focused on the mechanism of reaction of this reagent and its use in the design of biocatalysts, where genipin plays a double role, as a support activating agent and as inter- or intramolecular crosslinker. Its low toxicity makes this compound an ideal alterative to glutaraldehyde in these processes. Moreover, in some cases the features of the biocatalysts prepared using genipin surpassed those of the biocatalysts prepared using other standard crosslinkers, even disregarding toxicity. In this way, genipin is a very promising reagent in the design of biocatalysts.

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Facile and green fabrication of biocatalytic chitosan beads by one-step genipin-mediated β-glucosidase immobilization for production of bioactive genistein

Cited by 9 publications

References 56 publications

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications

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