Facile and effective separation of polysaccharides and proteins from Cordyceps sinensis mycelia by ionic liquid aqueous two-phase system

Yan, Jing; Le, Hai; Pei, Juan Juan; Wang, Zhen Bin; Wu, Jianyong

doi:10.1016/j.seppur.2014.03.020

Cited by 67 publications

(45 citation statements)

References 28 publications

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“…This phenomenon could be attributed to the fact that increasing the crude extract concentration causes the aggregation of OA molecules and the enhancement of intra-molecular hydrogen bonds, which weaken the interactions between OA and water molecules. Moreover, excessive addition of crude extract caused accumulation at the interface, which negatively affected mass transfer [21]. These results suggest that the crude extract concentration should be 8%(w/w).…”

Section: Effects Of Crude Extract Concentration On the Partition Coefmentioning

confidence: 95%

Aqueous Two-phase Extraction of Oleanolic acid from Grape Skins and Its Antioxidant Activity

Zhang¹,

Zhang²,

Luo³

et al. 2015

JFNR

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The aim of the current study is to focus on separating the oleanolic acid (OA) from previously prepared aqueous two-phase extraction (ATPE) of grape skins. Several different influential extraction parameters, such as ethanol concentration (v/w), ammonium sulphate concentration (w/w), crude extract concentration (w/w), extraction temperature, and pH, were also investigated. The optimal differential partitioning of OA was achieved in a system (at pH4.0, temperature=25°C)composed of 23% (v/w) ethanol, 18% (w/w) ammonium sulphate, 8% (w/w) crude extract and 41% (w/w) water. The recovery of extracted OA from experiments was determined to be 93.54%. The antioxidant activity of the separated ATPE in relative to VC were, also, investigated in this study at the proper conditions.The ATPE extract showed a relatively high antioxidant ability compared with that of Vitamin C. This proposed extraction technique opens up new possibilities in extraction of other active ingredients in natural plants or biologic samples.

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Section: Effects Of Crude Extract Concentration On the Partition Coefmentioning

confidence: 95%

Aqueous Two-phase Extraction of Oleanolic acid from Grape Skins and Its Antioxidant Activity

Zhang¹,

Zhang²,

Luo³

et al. 2015

JFNR

View full text Add to dashboard Cite

show abstract

“…Most polysaccharides were enriched into the salt‐rich phase, while the majority of proteins were extracted into IL‐rich phase. [C 4 mim][BF 4 ] or [C 4 mim][Cl] based ATPS showed good performance for the purification of polysaccharides from water extracts of plant . With [C 4 mim][Cl]‐based ATPS, the mannan and protein species in mixtures could be separated effectively and their extraction efficiency were 95–100 and 92–97% in a one‐step process, respectively .…”

Section: Introductionmentioning

confidence: 99%

A novel strategy to utilize ethylene glycol‐ionic liquids for the selective precipitation of polysaccharides

Zhao

et al. 2019

J of Separation Science

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In the present work, three hydrophilic ionic liquids based on the combination between imidazolium cations attached with ethylene glycol polymers of various lengths and hexafluorophosphate anion were designed and synthesized for the separation of polysaccharides. By employing dextran 100 kDa as model compound, the effects of ionic liquid content, solvent/anti‐solvent volume, and temperature on its recovery efficiency were investigated systematically. The ability of these ionic liquids to precipitate dextran 100 kDa, increases with the elongation of ethylene glycol polymer chain. The established ionic liquid‐based precipitation system was successfully applied to selectively precipitate polysaccharides from water extracts of three traditional Chinese medicines and the precipitation could be achieved in about 15 min. In addition, the different precipitation responses of acidic, neutral, and basic polysaccharides in the ionic liquid‐based precipitation system and theoretical calculations both suggested that the selective precipitation of polysaccharides was probably mediated by interaction between ionic liquids and polysaccharides. The proposed strategy facilitated the isolation and purification of polysaccharides and may trigger a novel application of ionic liquids in carbohydrate research.

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“…However, most polymers used to form the phases have high viscosity and form turbid solutions, interfering in determination of the analytes. In recent years it has been demonstrated that ionic liquids are a viable alternative for ATPS composed of common polymers since they present unique advantages, such as low viscosity, formation of emulsion, absence of volatile organic solvents, rapid phase separation and high extraction efficiency (Yan et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Thermophysical Properties of 1-Ethyl-3-Methylimidazolium Chloride Solution From 293.15 to 323.15 K

Gandolfi

Gonçalves

Pimentel

et al. 2019

Braz. J. Chem. Eng.

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Aqueous systems composed of ionic liquid make up a new alternative for use in processes involving the separation of biomolecules. The objective of this experiment was to obtain the thermo-physical properties of density, refractive index, electrical conductivity, molar volume, thermal expansion coefficient and apparent specific volume of the ionic liquid 1-ethyl-3-methylimidazolium chloride. The thermo-physical properties of aqueous solutions of this ionic liquid were measured as a function of the mass fraction w = (0.05, 0.125, 0.2, 0.275 and 0.35), temperature T = (293.15, 303.15, 313.15 and 323.15) K and pH = (7.5, 8.0 and 8.5). Models representing the combined effects between variables were fit since they are required for industrial applications where the physical parameters must be accurately calculated. Models representing the combined effects of the variables temperature, mass fraction and pH values of ionic liquid were adjusted and presented good fit.

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Facile and effective separation of polysaccharides and proteins from Cordyceps sinensis mycelia by ionic liquid aqueous two-phase system

Cited by 67 publications

References 28 publications

Aqueous Two-phase Extraction of Oleanolic acid from Grape Skins and Its Antioxidant Activity

Aqueous Two-phase Extraction of Oleanolic acid from Grape Skins and Its Antioxidant Activity

A novel strategy to utilize ethylene glycol‐ionic liquids for the selective precipitation of polysaccharides

Thermophysical Properties of 1-Ethyl-3-Methylimidazolium Chloride Solution From 293.15 to 323.15 K

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