Facial Skin Microbiota-Mediated Host Response to Pollution Stress Revealed by Microbiome Networks of Individual

Wang, Lu; Xu, Yining; Chu, Chung-Ching; Jing, Zehua; Chen, Yabin; Zhang, Jingsong; Pu, Mingming; Mi, Tingyan; Du, Yaping; Liang, Zong-Qi; Doraiswamy, Chandraprabha; Zeng, Tao; Wu, Jiarui; Chen, Luonan

doi:10.1128/msystems.00319-21

Cited by 8 publications

(7 citation statements)

References 39 publications

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“…Single sample networks were produced following methodologies described previously 27 . The production of n-1 networks facilitates the analysis of networks at the subject level and additionally allows improved statistical analysis of associated network metrics.…”

Section: Resultsmentioning

confidence: 99%

“…It is well documented that a high level of inter-individual variation occurs in the human skin microbiome in particular with regards to less dominant community members 4 . Recently, methodologies have been developed to examine microbiome networks at an individual level to facilitate the examination of an individual’s response to intervention 27 . Examination of single sample networks showed that all study subjects showed improvement in commonly used network analysis metrics including mean degree, edge number, node number and network robustness.…”

Section: Discussionmentioning

confidence: 99%

“…Networks for individual samples within the population were calculated using the microbiome network of an individual (MNI) method 53 . Networks generated via this method with an individual sample removed, are subtracted from the MNPs of the whole sample set, resulting in a network specific for that sample.…”

Section: Methodsmentioning

confidence: 99%

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Alteration of barrier properties, stratum corneum ceramides and microbiome composition in response to lotion application on cosmetic dry skin

Murphy

Grimshaw

Hoptroff

et al. 2022

Sci Rep

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Xerosis, commonly referred to as dry skin, is a common dermatological condition affecting almost a third of the population. Successful treatment of the condition traditionally involves the application of cosmetic products facilitating the moisturisation of the skin with a range of ingredients including glycerol and fatty acids. While the effectiveness of these treatments is not in question, limited information exists on the impact on the skin microbiome following use of these products and the improvement in skin hydration. Here, we describe improvements in skin barrier properties together with increased levels of cholesterol, ceramides and long-chain fatty acids following application of Body Lotion. Concomitant alterations in the skin microbiome are also seen via 16S rRNA metataxonomics, in combination with both traditional and novel informatics analysis. Following 5 weeks of lotion use, beneficial skin bacteria are increased, with improvements in microbiome functional potential, and increases in pathways associated with biosynthesis of multiple long chain fatty acids.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Alteration of barrier properties, stratum corneum ceramides and microbiome composition in response to lotion application on cosmetic dry skin

Murphy

Grimshaw

Hoptroff

et al. 2022

Sci Rep

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“…Co-occurrence network analyses based on metagenomic and amplicon sequences have been frequently used to infer microbial relations in environmental or host microbiomes, and further predict microbial interactions (positive or negative) in these environments 9 , 23 , 24 , 40 , 41 , which are informatic for discovering the true microbial interactions attributing to either microbial properties (such as metabolic coupling, nutrient competition, etc.) or from environmental filtration (such as responding similarly to environmental factors or due to physical separation) 42 , 43 .…”

Section: Discussionmentioning

confidence: 99%

Droplet microfluidics-based high-throughput bacterial cultivation for validation of taxon pairs in microbial co-occurrence networks

Jiang

Zhu

Zhou

et al. 2022

Sci Rep

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Co-occurrence networks inferred from the abundance data of microbial communities are widely applied to predict microbial interactions. However, the high workloads of bacterial isolation and the complexity of the networks themselves constrained experimental demonstrations of the predicted microbial associations and interactions. Here, we integrate droplet microfluidics and bar-coding logistics for high-throughput bacterial isolation and cultivation from environmental samples, and experimentally investigate the relationships between taxon pairs inferred from microbial co-occurrence networks. We collected Potamogeton perfoliatus plants (including roots) and associated sediments from Beijing Olympic Park wetland. Droplets of series diluted homogenates of wetland samples were inoculated into 126 96-well plates containing R2A and TSB media. After 10 days of cultivation, 65 plates with > 30% wells showed microbial growth were selected for the inference of microbial co-occurrence networks. We cultivated 129 bacterial isolates belonging to 15 species that could represent the zero-level OTUs (Zotus) in the inferred co-occurrence networks. The co-cultivations of bacterial isolates corresponding to the prevalent Zotus pairs in networks were performed on agar plates and in broth. Results suggested that positively associated Zotu pairs in the co-occurrence network implied complicated relations including neutralism, competition, and mutualism, depending on bacterial isolate combination and cultivation time.

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“…A higher rate of horizontal gene transfer is also linked to the industrialization era, which might facilitate acquiring of new functions and adaptation to the change in the environment (Groussin et al, 2021;Lau et al, 2021). A pioneering study shows that the skin microbiome is altered in the urban population and this alteration changes the physiological response of the skin and is implicated in skinrelated diseases (Lau et al, 2021;Wang et al, 2021c).…”

Section: Urbanization and Microbiome Compositionmentioning

confidence: 99%

Recent Advances in Understanding the Structure and Function of the Human Microbiome

2022

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Trillions of microbes live within our bodies in a deep symbiotic relationship. Microbial populations vary across body sites, driven by differences in the environment, immunological factors, and interactions between microbial species. Major advances in genome sequencing enable a better understanding of microbiome composition. However, most of the microbial taxa and species of the human microbiome are still unknown. Without revealing the identity of these microbes as a first step, we cannot appreciate their role in human health and diseases. A shift in the microbial balance, termed dysbiosis, is linked to a broad range of diseases from simple colitis and indigestion to cancer and dementia. The last decade has witnessed an explosion in microbiome research that led to a better understanding of the microbiome structure and function. This understanding leads to potential opportunities to develop next-generation microbiome-based drugs and diagnostic biomarkers. However, our understanding is limited given the highly personalized nature of the microbiome and its complex and multidirectional interactions with the host. In this review, we discuss: (1) our current knowledge of microbiome structure and factors that shape the microbial composition, (2) recent associations between microbiome dysbiosis and diseases, and (3) opportunities of new microbiome-based therapeutics. We analyze common themes, promises, gaps, and challenges of the microbiome research.

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Facial Skin Microbiota-Mediated Host Response to Pollution Stress Revealed by Microbiome Networks of Individual

Abstract: The association between the skin microbiome and skin health has been widely reported. However, the role of the skin microbiome in mediating skin physiology remains a challenging and yet priority subject in the field.

Cited by 8 publications

References 39 publications

Alteration of barrier properties, stratum corneum ceramides and microbiome composition in response to lotion application on cosmetic dry skin

Alteration of barrier properties, stratum corneum ceramides and microbiome composition in response to lotion application on cosmetic dry skin

Droplet microfluidics-based high-throughput bacterial cultivation for validation of taxon pairs in microbial co-occurrence networks

Recent Advances in Understanding the Structure and Function of the Human Microbiome

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