Facial Skeletal Augmentation Using Hydroxyapatite Cement

Shindo, Maisie L.; Costantino, Peter D.; Friedman, Craig D.; Chow, Laurence C.

doi:10.1001/archotol.1993.01880140069012

Cited by 178 publications

(132 citation statements)

References 28 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the CPC scaffold is advantageous over sintered hydroxyapatite implants, because CPC can self-harden in situ and conform to complex cavity shapes, while sintered hydroxyapatite requires machining which is difficult due to its brittleness. In addition, CPC can be resorbed and replaced by new bone [9,12,33], while sintered hydroxyapatite remains more stable.…”

Section: Discussionmentioning

confidence: 99%

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

Simon

2004

Journal Orthopaedic Research

View full text Add to dashboard Cite

Calcium phosphate cement (CPC) sets in situ to form solid hydroxyapatite, can conform to complex cavity shapes without machining, has excellent osteoconductivity, and is able to be resorbed and replaced by new bone. Therefore, CPC is promising for craniofacial and orthopaedic repairs. However, its low strength and lack of macroporosity limit its use. This study investigated CPC reinforcement with absorbable fibers, the effects of fiber volume fraction on mechanical properties and macroporosity, and the cytotoxicity of CPC–fiber composite. The rationale was that large‐diameter absorbable fibers would initially strengthen the CPC graft, then dissolve to form long cylindrical macropores for colonization by osteoblasts. Flexural strength, work‐of‐fracture (toughness), and elastic modulus were measured vs. fiber volume fraction from 0% (CPC Control without fibers) to 60%. Cell culture was performed with osteoblast‐like cells, and cell viability was quantified using an enzymatic assay. Flexural strength (mean ± SD; n == 6) of CPC with 60% fibers was 13.5 ± 4.4 MPa, three times higher than 3.9 ± 0.5 MPa of CPC Control. Work‐of‐fracture was increased by 182 times. Long cylindrical macropores 293 ± 46 μm in diameter were created in CPC after fiber dissolution, and the CPC–fiber scaffold reached a macroporosity of 55% and a total porosity of 81%. The new CPC–fiber formulation supported cell adhesion, proliferation and viability. The method of using large‐diameter absorbable fibers in bone graft for mechanical properties and formation of long cylindrical macropores for bone ingrowth may be applicable to other tissue engineering materials. Published by Elsevier Ltd. on behalf of Orthopuedic Research Society. © 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

show abstract

Section: Discussionmentioning

confidence: 99%

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

Simon

2004

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…[36][37][38][39] CPC is comprised of a mixture of tetracalcium phosphate [TTCP: Ca 4 (PO 4 ) 2 O] and dicalcium phosphate anhydrous (DCPA: CaHPO 4 ), which forms resorbable HA. 36,40,41 Due to its excellent osteoconductivity, CPC was approved in 1996 by the Food and Drug Administration for repairing craniofacial defects, thus becoming the first CPC available for clinical use. 40 However, the brittleness of CPC limits its use to only nonload-bearing areas.…”

mentioning

confidence: 99%

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Thein-Han

2011

Tissue Engineering Part A

View full text Add to dashboard Cite

Human umbilical cord mesenchymal stem cells (hUCMSCs) avoid the invasive procedure required to harvest bone marrow MSCs. The addition of collagen fibers into self-setting calcium phosphate cement (CPC) may increase the scaffold strength, and enhance cell attachment and differentiation. The objectives of this study were to develop a novel class of collagen-CPC composite scaffolds, and to investigate hUCMSC attachment, proliferation, and osteogenic differentiation on collagen-CPC scaffolds for the first time. Collagen fibers in CPC improved the load-bearing capability. Flow cytometry showed that the hUCMSCs expressed cell surface markers characteristic of MSCs, and were negative for hematopoietic and endothelial cell markers. hUCMSCs proliferated rapidly in all CPC composite scaffolds, with cell number increasing by sevenfold in 8 days. Cellular function was enhanced with collagen fibers in CPC scaffolds. Cell density increased from (645 -60) cells/mm 2 on CPC with 0% collagen, to (1056 -65) cells/mm 2 on CPC with 8% collagen ( p < 0.05). The actin stress fibers inside the hUCMSCs were stained, and the fluorescence intensity was doubled when the collagen in CPC was increased by 0% to 8%. RT-PCR showed that hUCMSCs on CPC with collagen had higher osteogenic expression than those on CPC without collagen. Alizarin Red S staining revealed a great increase in mineralization by hUCMSCs on CPC with collagen than that without collagen. In conclusion, hUCMSCs showed excellent proliferation, differentiation, and synthesis of bone minerals in collagen-CPC composite scaffolds for the first time. The novel hUCMSC-seeded collagen-CPC construct with superior cell function and load-bearing capability is promising to enhance bone regeneration in a wide range of orthopedic and craniofacial applications.

show abstract

“…In addition, it may be used in the sinus region for facial augmentation [127,562] and the formulation can be supported by metal hardware [127]. In dogs, BoneSource TM was employed to supplement the supraorbital ridge and to augment skull base defects [563]. Another group performed trials to ascertain the inflammation around the site and the degree of loss of the implanted BoneSource TM .…”

Section: Oral Maxillofacial and Craniofacial Applicationsmentioning

confidence: 99%

Self-Setting Calcium Orthophosphate Formulations

Dorozhkin¹

2012

Calcium Orthophosphates

View full text Add to dashboard Cite

Abstract:In early 1980s, researchers discovered self-setting calcium orthophosphate cements, which are bioactive and biodegradable grafting bioceramics in the form of a powder and a liquid. After mixing, both phases form pastes, which set and harden forming either a non-stoichiometric calcium deficient hydroxyapatite or brushite. Since both of them are remarkably biocompartible, bioresorbable and osteoconductive, self-setting calcium orthophosphate formulations appear to be promising bioceramics for bone grafting. Furthermore, such formulations possess excellent molding capabilities, easy manipulation and nearly perfect adaptation to the complex shapes of bone defects, followed by gradual bioresorption and new bone formation. In addition, reinforced formulations have been introduced, which might be described as calcium orthophosphate concretes. The discovery of self-setting properties opened up a new era in the medical application of calcium orthophosphates and many commercial trademarks have been introduced as a result. Currently such formulations are widely used as synthetic bone grafts, with several advantages, such as pourability and injectability. Moreover, their low-temperature setting reactions and intrinsic porosity allow loading by drugs, biomolecules and even cells for tissue engineering purposes. In this review, an insight into the self-setting calcium orthophosphate formulations, as excellent bioceramics suitable for both dental and bone grafting applications, has been provided.

show abstract

Facial Skeletal Augmentation Using Hydroxyapatite Cement

Cited by 178 publications

References 28 publications

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Self-Setting Calcium Orthophosphate Formulations

Contact Info

Product

Resources

About