Fabrication, structure and surface charges of albumin-chitosan hybrids

He, Naipu; Wang, Rong‐Min; He, Yufeng; Dang, XiMei

doi:10.1007/s11426-012-4604-z

Cited by 14 publications

(9 citation statements)

References 50 publications

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“…The relatively large difference in the spectral change at 1669 cm −1 (due to the C=O group) for the C(2:1) samples compared to the C(3:1) samples suggests that conjugate formation depended on the ratio of LF to HA used. This result was in agreement with previous findings suggesting that HA was grafted to the LF partially via the formation of a new amide bond [18,20].…”

Section: Resultssupporting

confidence: 93%

“…On the other hand, SDS was unable to disrupt the covalent amide bonds holding the LF and HA molecules together. A similar phenomenon has been reported by He et al [18], who showed that the formation of an amide bond between bovine serum albumin and chitosan could be achieved using EDC as a coupling reagent.…”

Section: Resultssupporting

confidence: 84%

“…EDC is widely used as a carboxyl activating agent to promote the formation of amide bonds in proteins [18]. In our study, EDC was used to initiate the reactive carboxyl groups on HA, and NHS was used to increase the efficiency of the EDC coupling reaction.…”

Section: Resultsmentioning

confidence: 99%

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Formation and Characterization of Lactoferrin-Hyaluronic Acid Conjugates and Their Effects on the Storage Stability of Sesamol Emulsions

et al. 2018

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The purpose of this study was to fabricate biopolymer conjugates from lactoferrin (LF) and hyaluronic acid (HA) and then to investigate their potential as emulsifiers for forming sesamol-loaded emulsions. Initially, LF-HA covalent conjugates were formed using the carbodiimide coupling method in aqueous solutions at pH = 4.5, and then the nature of the conjugates was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy, and fluorescence spectroscopy. The results demonstrated the formation of an amide link between the amine groups of LF and the carboxyl groups of HA. Sesamol emulsions were prepared using the LF-HA conjugates as emulsifiers and their stability was determined. The conjugates improved both the physical and chemical stability of the emulsions during storage. Optimum stability of the emulsion was obtained at a LF-to-HA molar ratio of 2:1. Our results suggest that LF-HA conjugates may be effective emulsifiers for use in food stuffs and other applications.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 84%

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Formation and Characterization of Lactoferrin-Hyaluronic Acid Conjugates and Their Effects on the Storage Stability of Sesamol Emulsions

et al. 2018

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“…NHS was used to enhance coupling efficiency of EDC. 51 The unreacted Tf was quantified spectrophotometrically at λ max = 280 nm using the equation obtained from the standard curve of Tf. The results demonstrated that about 80% of Tf was conjugated to chitosan.…”

Section: Resultsmentioning

confidence: 99%

Transferrin-targeted poly(butylene adipate)/terephthalate nanoparticles for targeted delivery of 5-fluorouracil in HT29 colorectal cancer cell line

Varshosaz

Riahi

Ghassami

et al. 2017

Journal of Bioactive and Compatible Polymers

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The purpose of this study was to design 5-fluorouracil-loaded poly(butylene adipate)/terephthalate (Ecoflex®) nanoparticles for targeting colorectal cancer. The nanoparticles were prepared by emulsification–solvent evaporation method and optimized by a full factorial design. The effects of polymer and surfactant concentration, surfactant type, and stirrer rate were studied on the particle size, zeta potential, loading efficiency, and release efficiency of nanoparticles. For production of targeted nanoparticles, chitosan was conjugated to transferrin which was then coated on the surface of Ecoflex nanoparticles via electrostatic interactions. The conjugation of transferrin/chitosan was verified by Fourier transform infrared spectroscopy, ultraviolet spectroscopy, and SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) methods and quantified by ultraviolet spectroscopy assay. The cytotoxicity of 5-fluorouracil loaded in targeted and non-targeted nanoparticles was studied on human colon adenocarcinoma cell line (HT29), Michigan Cancer Foundation-7 (MCF-7), and human umbilical vein endothelial cells using MTT (thiazolyl blue tetrazolium bromide) assay. The best results were obtained from nanoparticles prepared by 0.2% of the polymer, 2% of Tween 20, and stirrer speed of 17,500 r/min. The successful conjugation of transferrin/chitosan was confirmed by Fourier transform infrared spectrum and SDS-PAGE results and was about 80%. The targeted nanoparticles showed significantly more cytotoxic effects on HT29 cells compared to free 5-fluorouracil and non-targeted nanoparticles. Blocking transferrin receptors resulted in a significantly higher cell survival for targeted nanoparticles which confirmed receptor-mediated cellular uptake of targeted nanoparticles.

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“…An EDC-mediated reaction was carried out to conjugate Tf with PAH through formation of amide linkages between COOH groups of Tf and NH 2 groups of PAH. 46 Briefly, a solution of Tf (10 mg) in 10 mM phosphate buffer solution at pH 7.4 was prepared, followed by addition of EDC (24 mg) and NHS ( the coupling reagents to activate the carboxyl groups of Tf. The resulting solution was gently stirred for 2 h under nitrogen atmosphere at 10 °C in darkness.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Targeted Delivery of Docetaxel by Use of Transferrin/Poly(allylamine hydrochloride)-functionalized Graphene Oxide Nanocarrier

Nasrollahi

Varshosaz²,

Khodadadi

et al. 2016

ACS Appl. Mater. Interfaces

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The exceptional chemical and physical properties of graphene oxide (GO) make it an attractive nanomaterial for biomedical applications, particularly in drug delivery. In this work we synthesized a novel, GO-based nanocarrier for the delivery of docetaxel (DTX), a potent hydrophobic chemotherapy drug. The GO was functionalized with transferrin (Tf)-poly(allylamine hydrochloride) (PAH), which provided targeted and specific accumulation to extracellular Tf receptors and stabilized GO in physiological solutions. Tf was conjugated to PAH via amide covalent linkages, and Tf-PAH coated the surface of DTX-loaded GO through electrostatic interactions. The morphology and structure of the resulting nanostructure, along with its surface modifications, were verified by use of Fourier transform infrared (FT-IR) and UV-vis spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). DTX was loaded at a relatively high loading capacity of 37% and released in a pH-dependent and sustained manner under physiological conditions. The targeting efficiency and cytotoxicity of this drug delivery system were evaluated on MCF-7 breast cancer cells. Improved efficacy of targeted DTX-loaded nanocarrier was observed compared to nontargeted carrier and free DTX, especially at high drug concentrations. The Tf-PAH-functionalized GO nanocarrier is a promising candidate for targeted delivery and controlled release of DTX.

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Fabrication, structure and surface charges of albumin-chitosan hybrids

Cited by 14 publications

References 50 publications

Formation and Characterization of Lactoferrin-Hyaluronic Acid Conjugates and Their Effects on the Storage Stability of Sesamol Emulsions

Formation and Characterization of Lactoferrin-Hyaluronic Acid Conjugates and Their Effects on the Storage Stability of Sesamol Emulsions

Transferrin-targeted poly(butylene adipate)/terephthalate nanoparticles for targeted delivery of 5-fluorouracil in HT29 colorectal cancer cell line

Targeted Delivery of Docetaxel by Use of Transferrin/Poly(allylamine hydrochloride)-functionalized Graphene Oxide Nanocarrier

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