Fabrication of UV-crosslinked chitosan scaffolds with conjugation of RGD peptides for bone tissue engineering

Tsai, Wei‐Bor; Chen, Yi-Ru; Liu, Hsuan‐Liang; Lai, Juin‐Yih

doi:10.1016/j.carbpol.2011.02.003

Cited by 66 publications

(32 citation statements)

References 31 publications

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“…Based on the previous studies, the difference here was probably caused by the RGD. RGD in the scaffold affects cell adhesion, which indirectly causes better cell proliferation and growth [11]. This was consistent with a previous study that used a chitosan scaffold with RGD immobilization.…”

Section: Discussionsupporting

confidence: 92%

“…This result was also supported by other studies that indicated that modification of RGD on the surface of nanofiber chitosan could significantly improve fibroblast adhesion to the nanofiber chitosan itself [20]. Another study reported that RGD immobilization to a chitosan scaffold showed an even better ability to support osteoblast growth in cultures [11,14]. From these studies, it can be concluded that combining a chitosan scaffold with RGD can improve cell growth, so the hypothesis states that RCSSS in the form of a membrane is not toxic to human dental pulp cells can be accepted.…”

Section: Discussionsupporting

confidence: 77%

“…One way to improve the cell adhesion with a chitosan scaffold is to combine the scaffold with Arg-Gly-Asp (RGD). According to the previous studies, adding RGD to a chitosan scaffold supported scaffold adhesion to tissue [11].…”

Section: Discussionmentioning

confidence: 92%

“…As an attempt to improve its role as a scaffold, chitosan can be combined with other biomaterials, such as Arg-Gly-Asp (RGD) [11]. RGD is a peptide linked to cell adhesion and also plays a role in tissue regeneration by guiding cell migration [12].…”

Section: Introductionmentioning

confidence: 99%

“…RGD is commonly found in various bone proteins, such as fibronectin, vitronectin, fibrinogen, osteopontin, and bone sialoprotein [13]. Based on the previous studies, adding or combining RDG into a chitosan scaffold was reported to improve the biocompatibility of that chitosan [11,14]. Currently, the Indonesia National Atomic Energy Agency (BATAN) has been developing chitosan scaffolds from shrimp shells in the form of a membrane combined with RGD.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity Analysis of RGD-Chitosan From Shrimp Shell Scaffold Membranes Toward Human Dental Pulp Cells

et al. 2017

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Objective:This study aimed to analyze RGD-Chitosan from Shrimp Shells' Scaffolds' (RCSSS) and CSSS membrane toxicity toward human dental pulp cells. Methods:Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determined using an MTT assay method.Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSS group 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells compared to CSSS membranes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toxicity Analysis of RGD-Chitosan From Shrimp Shell Scaffold Membranes Toward Human Dental Pulp Cells

et al. 2017

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Developments in Hydrogel‐based Scaffolds and Bioceramics for Bone Regeneration

Stancu

Chappard

2019

Bioceramics and Biocomposites

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Liposomes‐in‐Chitosan Hydrogels: Challenges and Opportunities for Biomedical Applications

Grijalvo

Eritja

Díaz

2019

Materials Science and Technology

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4 drug delivery (17) and tissue engineering (e.g. bone (18), cartilage (18,19) or neural (20) tissue regenerations). From the structural point of view, CS is a linear polysaccharide made of randomly distributed mixtures of deacetylated residues (β-(14)-linked-D-glucosamine) and acetylated residues (N-acetyl-Dglucosamine). This polysaccharide is commercially produced by deacetylation of chitin ( Figure 1A). The percentage of deacetylated and acetylated residues (so-called degree of deacetylation, DA) in CS determines its physicochemical properties as well as solubility (21), biodegradability and biological activity (22).The protonation of the amino groups on the CS backbone under acidic conditions permits the biopolymer solubility before reaching pH values that can range from 6.2 and 6.5. As a consequence, the amino groups of the Dgalactosamine units are predominantly positively charged at pH values below 6.2 (23, 24). The development of physical entanglements in CS hydrogels is governed by reversible non-covalent forces such as hydrogen, ionic and/or hydrophobic intermolecular interactions that depend on the pH, temperature, chemical composition and polymer chain length (24,25,26). Importantly, the robustness of CS-based hydrogels permits the incorporation of small molecules during the gelation process under mild conditions. In this regard, β-glycerophosphate (GP) has been described as an efficient catalyst to trigger the CS sol-to-gel transition at physiological pH. This process has led to transparent physically cross-linked hydrogels with potential use as injectable biomaterials (24) ( Figure 1B). Other small anionic molecules have also been described as effective ionic cross-linking agents including a) citrate and sulphates, b) transition metal ions like Pt (II) (27) or Mo (IV) (28), and c) the use

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Fabrication of UV-crosslinked chitosan scaffolds with conjugation of RGD peptides for bone tissue engineering

Cited by 66 publications

References 31 publications

Toxicity Analysis of RGD-Chitosan From Shrimp Shell Scaffold Membranes Toward Human Dental Pulp Cells

Toxicity Analysis of RGD-Chitosan From Shrimp Shell Scaffold Membranes Toward Human Dental Pulp Cells

Developments in Hydrogel‐based Scaffolds and Bioceramics for Bone Regeneration

Liposomes‐in‐Chitosan Hydrogels: Challenges and Opportunities for Biomedical Applications

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