Fabrication of Ultrathin, Protein-containing Films by Layer-by-Layer Assembly and Electrochemical Characterization of Hemoglobin Entrapped in the Film

Shang, Libin; Liu, Xinjian; Zhong, Jun; Chen, Fan; Suzuki, I.; Li, Genxi

doi:10.1246/cl.2003.296

Cited by 37 publications

(29 citation statements)

References 10 publications

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“…However, at Myb/ RTIL/Nafion/BPG electrode, a new cathodic peak appears at about À 0.810 V (vs. Ag/AgCl) in the negative direction of the redox peaks of Myb. According to the previous report [29], this peak corresponded to the reduction of NO, which might be facilitated by electroactive Myb. With increasing the NO concentration, the peak currents of Myb decreased.…”

Section: Electrocatalytic Reduction Of No On Myb/rtil/supporting

confidence: 59%

Direct Electrochemistry of Myoglobin on a Room Temperature Ionic Liquid Modified Electrode and Its Application to Nitric Oxide Biosensing

2008

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Myoglobin (Myb) was successfully immobilized on a room temperature ionic liquid (RTIL), 1-ethyl-3-methyl imidazolium tetrafluoroborate ([EMIM][BF 4 ]) modified basal plane graphite (BPG) electrode. The electrochemical behavior of Myb on RTIL modified BPG electrode was explored and the results from cyclic voltammetry (CV) showed a well-defined and quasi-reversible CV peaks with a formal potential of À 0.379 V (versus Ag/AgCl) in a phosphate buffer solution (pH 7.0). RTIL shows an obvious promotion for the direct electron-transfer between Myb and BPG electrode. Myb adsorbed on electrode surface exhibits an obvious electrocatalytic activity for the reduction of nitric oxide (NO). The catalytic current is corresponding linearly to the NO concentration in the range of 7.0 Â 10 À7 to 7.0 Â 10 À6 M with a limit of detection of 2.0 Â 10 À7 M (three times the ratio of signal to noise, S/N ¼ 3).

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Section: Electrocatalytic Reduction Of No On Myb/rtil/supporting

confidence: 59%

Direct Electrochemistry of Myoglobin on a Room Temperature Ionic Liquid Modified Electrode and Its Application to Nitric Oxide Biosensing

2008

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“…As can be seen, a new cathodic peak appears at about À 0.8 V (curve b). According to the previous report [58], this peak corresponds to the reduction of NO while the reaction was facilitated by the electroactive Mb. The current response was sensitive enough, suggesting the MWNTNafion-Mb film modified Ch/GCE could be used as a biosensor to detect the concentration of NO.…”

Section: Catalytic Reactivity Toward O 2 and H 2 Osupporting

confidence: 56%

“…The current response was sensitive enough, suggesting the MWNTNafion-Mb film modified Ch/GCE could be used as a biosensor to detect the concentration of NO. The possible reaction processes could be described in following equations [58]:…”

Section: Catalytic Reactivity Toward O 2 and H 2 Omentioning

confidence: 99%

Fabrication of a Nanobiocomposite Film Containing Heme Proteins and Carbon Nanotubes on a Choline Modified Glassy Carbon Electrode: Direct Electrochemistry and Electrochemical Catalysis

Lin

Jiang

2006

Electroanalysis

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A nanocomposite electrochemical sensing film is assembled on choline (Ch) modified glassy carbon electrode (GCE), which contains multiwalled carbon nanotubes (MWNTs), Nafion cation exchanger, and myoglobin (Mb) or hemoglobin (Hb). The MWNTs provide a 3D porous and conductive network for the enzyme immobilization and Nafion acts as polymeric binder to give cast thin films. Both MWNTs and Nafion provide negative functionalities to bind to the positively charged redox proteins and to attach at the positively charged Ch modified layer, and drive the formation of homogeneous and stable nanocomposite film, the MWNT-Nafion-Mb. The nanocomposite film was characterized by field emission scanning electron microscope (FE-SEM). The Mb in the nanocomposite film showed a pair of well-defined and nearly reversible cyclic voltammetric peaks at about À 0.32 V vs. SCE at pH 7.0 solution for the heme Fe(III)/Fe(II) redox couple. The immobilized heme proteins can display the features of peroxidase in electrocatalytic reductions of oxygen, hydrogen peroxide, nitric oxide, trichloroacetic acid (TCA), and bromate.

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“…[20][21][22][23] The results are in good agreement with the characteristics of the heme Fe(III)/Fe(II) redox couples. While no voltammetric peak appeared at bare CILE or PVA/Clay/CILE.…”

Section: Electrochemical Behaviors Of Pva/clay/hb/cilesupporting

confidence: 78%

Direct electrochemistry of hemoglobin immobilized in polyvinylalcohol and clay composite film modified carbon ionic liquid electrode

Hui¹,

Gao²,

Li³

et al. 2009

J. Braz. Chem. Soc.

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Neste trabalho, líquido iônico hexaflúor-fosfato de 1-butil-3-metilimidazólio (BMIMPF 6 ), em temperatura ambiente, foi usado como ligante na fabricação de um eletrodo líquido iônico de carbono (CILE). Hemoglobina (Hb) foi imobilizada na superfície de CILE com argila e um filme composto por polivinil álcool (PVA) pelo método camada por camada. Espectros de UV-Vis e FT-IR mostraram que Hb no filme reteve as características essenciais de sua estrutura original. In this paper room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6 ) was used as binder to fabricate a carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on the surface of CILE with clay and polyvinyl alcohol (PVA) composite film by layer to layer method. UV-Vis and FT-IR spectra showed that Hb in the film retained the essential features of its native structure. Electrochemical experiments indicated that a pair of well-defined quasi-reversible redox peak was obtained in pH 7.0 Britton-Robinson (B-R) buffer solution. The reduction and oxidation peak potentials were located at −0.405 V and −0.274 V (vs. SCE) with the formal potential as −0.340 V, which was contributed to the electrochemical reaction of heme Fe(III)/Fe(II) redox couples. The results suggested that the direct electron transfer of Hb in the PVA/Clay film with the CILE was accomplished. The direct electrochemical behaviors of Hb were carefully studied with the electrochemical parameters calculated. The PVA/ Clay/Hb modified CILE gave excellent electrocatalytic ability to the reduction of H 2 O 2 and the apparent Michaelis-Menten constant (K M app ) value of Hb in the PVA/Clay film was calculated as 56.26 μmol L -1 .

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Fabrication of Ultrathin, Protein-containing Films by Layer-by-Layer Assembly and Electrochemical Characterization of Hemoglobin Entrapped in the Film

Cited by 37 publications

References 10 publications

Direct Electrochemistry of Myoglobin on a Room Temperature Ionic Liquid Modified Electrode and Its Application to Nitric Oxide Biosensing

Direct Electrochemistry of Myoglobin on a Room Temperature Ionic Liquid Modified Electrode and Its Application to Nitric Oxide Biosensing

Fabrication of a Nanobiocomposite Film Containing Heme Proteins and Carbon Nanotubes on a Choline Modified Glassy Carbon Electrode: Direct Electrochemistry and Electrochemical Catalysis

Direct electrochemistry of hemoglobin immobilized in polyvinylalcohol and clay composite film modified carbon ionic liquid electrode

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