Peptide-adsorption studies in the biomaterials field are typically performed in phosphate-buffered saline (PBS) to represent the physiological environment. However, the study of peptide adsorption in pure water or low-salt media (e.g., 10 mM potassium phosphate buffered water; PPB) is of interest for many other applications in the broader field of biotechnology. In previous studies we used surface plasmon resonance spectroscopy (SPR) and atomic force microscopy (AFM) to determine the standard state adsorption free energy of peptide adsorption (ΔG°ads) and the force required for peptide desorption (Fdes), respectively, for a wide range of surface chemistries in PBS and showed that these two parameters were strongly correlated. The objective of this present research was to repeat these studies in PPB rather than in PBS in order to determine the influence of the differences in salt concentration between these two environments and also to determine if the same correlation between ΔG°ads and Fdes held for peptide adsorption in PPB. The results from these studies show that ΔG°ads and the correlation between ΔG°ads and Fdes in PPB are not significantly different than in PBS for the evaluated set of peptide-surface systems.