Fabrication of Robust Biomolecular Patterns by Reactive Microcontact Printing onN-Hydroxysuccinimide Ester-Containing Polymer Films

Reactive Microcontact Printing on Block Copolymer Films: Exploiting Chemistry in Microcontacts for Sub‐micrometer Patterning of Biomolecules

“…The chemical contrast is in full agreement with previously reported data for a related polymer platform and data obtained on functionalized PS 690 -b-PtBA 1210 films in micrometer-scale patterns. [17,23] No evidence for pronounced TFA diffusion was observed at these length scales, indicating that the minimum feature size is limited by the feature dimensions on the elastomeric stamp at this point. On the nanopatterned substrates exposing 300 nm wide lines of fibronectin and 500 nm wide lines of PEG 5000 , the cells were observed to spread in highly unusual very elongated shapes.…”

“…After peeling off the stamp, the films were rinsed three times using Milli-Q water, activated with NHS/ EDC (i.e, treated in an aqueous solution of EDC (1 M) and NHS (0.2 M) for 30 min, and rinsed with Milli-Q water), and reacted with fluoresceinamine, PEG 5000 NH 2 , or fibronectin (100 lM in PB, pH 7.4). Before the direct transfer of PEG 5000 NH 2 (carried out according to a published procedure [23]), the polymer films were hydrolyzed in neat TFA for 20 min, rinsed three times using Milli-Q water, and activated with NHS/EDC. After soaking in the PEG 5000 NH 2 solution for 60 min, the stamp was loaded with an excess of PEG by solution-casting a PEG 5000 NH 2 solution onto the top of the stamp.…”

Graded‐Bandgap Quantum‐ Dot‐Modified Nanotubes: A Sensitive Biosensor for Enhanced Detection of DNA Hybridization

“…The amino-functionalized single-stranded DNA was covalently bonded on the inner walls of the NTs from a 1 lM aqueous solution (phosphate buffer (PB), pH 7.4) for 30 min, followed by rinsing with PB [18]. The aqueous solution containing Cy5-labeled target DNA (pH 7.4, 100 nM) was then applied to the NTs for 30 min at room temperature.…”

X-ray Photoelectron Spectroscopy, Time-of-Flight Secondary Ion Mass Spectrometry, and Principal Component Analysis of the Hydrolysis, Regeneration, and Reactivity of N-Hydroxysuccinimide-Containing Organic Thin Films

“…the apparent amine reactivity of NHS-bearing surfaces can be addressed. In a series of studies of surface structure -reactivity relationships, Schönherr et al 17,18,[20][21][22][23][24] applied quantitative methods, such as XPS and FTIR, to obtain chemical information about surface succinimidyl rings. However, XPS and FTIR do not discriminate chemically bound surface NHS moieties from reacted, surface-resident NHS moieties.…”