Fabrication of PVA-chitosan-based nanofibers for phytase immobilization to enhance enzymatic activity

Kamacı, Umran Duru; Peksel, Ayşegül

doi:10.1016/j.ijbiomac.2020.08.226

Cited by 41 publications

(17 citation statements)

References 40 publications

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“…PVA is also widely used in biomedical fields due to its non-toxic, hydrophilic, and biocompatible properties [123]. Related research studies [124][125][126] have described some cases of nanofibers made by electrospinning PVA and chitosan, whereby the aqueous PVA solution also removes potential organic solvent residues. As a result, antibacterial materials made of PVA and CS are considered harmless, are biocompatible, and offer easy adaptation for defined applications.…”

Section: Auxiliary Solvents and Compositesmentioning

confidence: 99%

Electrospinning of Chitosan for Antibacterial Applications—Current Trends

2021

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Chitosan is a natural biopolymer that can be suitable for a wide range of applications due to its biocompatibility, rigid structure, and biodegradability. Moreover, it has been proven to have an antibacterial effect against several bacteria strains by incorporating the advantages of the electrospinning technique, with which tailored nanofibrous scaffolds can be produced. A literature search is conducted in this review regarding the antibacterial effectiveness of chitosan-based nanofibers in the filtration, biomedicine, and food protection industries. The results are promising in terms of research into sustainable materials. This review focuses on the electrospinning of chitosan for antibacterial applications and shows current trends in this field. In addition, various aspects such as the parameters affecting the antibacterial properties of chitosan are presented, and the application areas of electrospun chitosan nanofibers in the fields of air and water filtration, food storage, wound treatment, and tissue engineering are discussed in more detail.

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Section: Auxiliary Solvents and Compositesmentioning

confidence: 99%

Electrospinning of Chitosan for Antibacterial Applications—Current Trends

2021

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“…This valid promotion of enzyme activity is perhaps based on the presence of Zn–Zr APHNs, which possess a larger surface area and a high charge carrier property that will accelerate the interaction between enzymes and TMB (Figure a). In addition, Zn and Zr could support another enzymatic catalysis site and promote the activity of Cyt-c to transform metalloenzymes without any difficult molecular designs. − A comparison of other materials (Table S2 ,,, ) with the Zn–Zr APHNs showed a relatively better adsorption capacity or catalysis activity of Zn–Zr APHNs. This superior capacity or catalysis activity promotion for enzymes has significance for practical applications.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, Zn and Zr could support another enzymatic catalysis site and promote the activity of Cyt-c to transform metalloenzymes without any difficult molecular designs. 36−38 A comparison of other materials (Table S2 10,11,13,39 ) with the Zn−Zr APHNs showed a relatively better adsorption capacity or catalysis activity of Zn−Zr APHNs. This superior capacity or catalysis activity promotion for enzymes has significance for practical applications.…”

Section: Resultsmentioning

confidence: 99%

Construction of Metal Hydrate-Based Amorphous Magnetic Nanosheets for Enhanced Protein Enrichment and Immobilization

Guo

Zhang

Jiao

et al. 2021

ACS Appl. Mater. Interfaces

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Inspired by the hierarchical fabrication technique, many self-assembly procedures have improved the construction of nanomaterials with unique physicochemical characteristics and multiple functions. The generation of multiple complexes is always accompanied by hierarchical structures and intriguing properties that are distinct from their individual segments. An interesting composite is amorphous magnetic Zn−Zr phosphate hydrated nanosheets (Zn−Zr APHNs), generated using templated synthesis and nanoparticle codeposition. The special porous structure of this construct, together with the abundance of metal ions and hydrate present, endows it with many interaction sites for proteins, provides high loading efficiency, and enhances bioactivity. Then, a series of proteins, including enzymes, was immobilized by the Zn−Zr APHNs by multiple interactions, high ionization, and larger surface of the nanosheets. In this study, novel methods for the enrichment of bioactive proteins while retaining the activity of protein payloads are presented. As a verification method, it is indicated that the Zn−Zr APHNs can deliver enzyme proteins (i.e., Cyt-c) to increase the catalytic activity with their biological function and structural integrity, resulting in a highly increased activity to free proteins.

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“…When used for metal ion adsorption filtration, water filtration, special gas filtration, a high temperature condition, and the extended drug release of wound dressings, PVA/CS membranes demand a specified processing, e.g., thermal cross-linking, hydrogen bond cross-linking, or solvent cross-linking, thereby attaining more general applications. However, the specified processing is usually accompanied with toxic solvents [76][77][78][79][80][81][82].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Needleless Electrospinning Polyvinyl Alcohol/Water-Soluble Chitosan Nanofibrous Membranes: Antibacterial Property and Filter Efficiency

et al. 2022

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Electrospinning is an efficient method of producing nanofibers out of polymers that shows a great potential for the filtration territory. Featuring water-soluble chitosan (WS-CS), a low-pollution process and a self-made needleless machine, PVA/WS-CS nanofibrous membranes were prepared and evaluated for nanofiber diameter, bacteriostatic property, filtration efficiency, pressure drop, and quality factor. Test results indicate that the minimal fiber diameter was 216.58 ± 58.15 nm. Regardless of the WS-CS concentration, all of the PVA/WS-CS nanofibrous membranes attained a high porosity and a high water vapor transmission rate (WVTR), with a pore size of 12.06–22.48 nm. Moreover, the membranes also exhibit bacteriostatic efficacy against Staphylococcus aureus, an optimal quality factor of 0.0825 Pa−1, and a filtration efficiency as high as 97.0%, that is 72.5% higher than that of common masks.

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Fabrication of PVA-chitosan-based nanofibers for phytase immobilization to enhance enzymatic activity

Cited by 41 publications

References 40 publications

Electrospinning of Chitosan for Antibacterial Applications—Current Trends

Electrospinning of Chitosan for Antibacterial Applications—Current Trends

Construction of Metal Hydrate-Based Amorphous Magnetic Nanosheets for Enhanced Protein Enrichment and Immobilization

Preparation of Needleless Electrospinning Polyvinyl Alcohol/Water-Soluble Chitosan Nanofibrous Membranes: Antibacterial Property and Filter Efficiency

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