Fabrication of protease XIV-loaded microspheres for cell spreading in silk fibroin hydrogels

Xiao, Wenqian; Zhang, Jing; Qu, Xiaohang; Chen, Ke; Gao, Haiming; He, Jisu; Ma, Tao; Li, Bo; Liao, Xiaoling

doi:10.1007/s10856-020-06466-7

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…188 Streptomyces griseus (XIV) protease porous microsphere loaded (CaCO 3 @HA) silk fibroin (SF) hydrogels constitute a multiscale delivery system that controls calcium ion release to promote long-term stability and osteogenic differentiation of MSCs. 189 Thus, ionic composite hydrogelbased multiscale delivery systems could be a promising therapeutic strategy in the field of IVD tissue engineering. In addition, ion-responsive hydrogels based on responsive Na + , K + , and Ba 2+ have been widely used in sensors and detectors 182,[190][191][192] and are expected to be promising strategies for regenerating IVD in the future.…”

Section: Ion-sensitive Hydrogelsmentioning

confidence: 99%

Aggressive strategies for regenerating intervertebral discs: stimulus-responsive composite hydrogels from single to multiscale delivery systems

Gao

Zhang

et al. 2022

J. Mater. Chem. B

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Section: Ion-sensitive Hydrogelsmentioning

confidence: 99%

Aggressive strategies for regenerating intervertebral discs: stimulus-responsive composite hydrogels from single to multiscale delivery systems

Gao

Zhang

et al. 2022

J. Mater. Chem. B

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“…In the future, chemical and biological cues can be introduced to the hydrogel to provide a suitable microenvironment for cell growth and proliferation. [44,45]…”

Section: Cell Encapsulation Experimentsmentioning

confidence: 99%

Conformational Transition‐Driven Self‐Folding Hydrogel Based on Silk Fibroin and Gelatin for Tissue Engineering Applications

Wang

Yan

Liu

et al. 2022

Macromolecular Bioscience

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Self‐folding is a rapidly evolving method for converting flat objects into three‐dimensional (3D) structures. However, because there are few materials with suitable properties, the application of self‐folding in tissue engineering has been hindered greatly. Herein, a novel self‐folding hydrogel using conformational transition mechanism is developed by employing photocrosslinkable silk fibroin and gelatin composite hydrogel. It is hypothesized that differences in the amount of beta‐sheet (β‐sheet) formation between the upper and lower layers will supply additional folding stress and drive the self‐folding behavior of a bilayer patch, which can improve the mechanical properties and long‐term stability of the self‐folded structure. In this study, the impact of various proportions of β‐sheets in composite hydrogels on their swelling, mechanics, and internal microstructures are investigated. Subsequently, the folding process parameters are optimized, and diffusion through the folded tubular structure is studied with a perfusion test. Finally, it is proven that the self‐folding hydrogel system is cytocompatible and can be utilized to build a 3D coculture system of “endothelial cells–smooth muscle cells”. These findings suggest that the self‐folding hydrogel can be a promising candidate for applications in blood vessel tissue engineering and regenerative medicine.

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“…Silk fibroin (SF) has been widely used as a protein biomaterial for the fabrication of membranes, porous scaffolds, hydrogels, and nanoparticles for a variety of biomedical purposes, credited to its biological compatibility, adjustable degradability, and strong mechanical characteristics. [1][2][3][4][5][6][7] Regenerated silk fibroin (RSF) possesses exceptional film-forming capabilities, and is also compatible with the human body. 8,9 In their hydrated form, SF films have a high level of dissolved oxygen permeability similar to that of human skin, suggesting potential applications for these films in artificial skin systems and wound dressing.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Incorporation of nanoparticles in silk fibroin solution: Toward water‐stable silk fibroin films possessing silk I structure

Tariq,

Zhu,

Tariq

et al. 2024

Polymers for Advanced Techs

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Water‐stable silk fibroin (SF) films are normally prepared by transforming the secondary structure of SF, from amorphous silk I (random coils) to silk II (β‐sheet), via physical or chemical means. Although mechanically strong, these films are generally brittle. Herein, we prepared water‐stable flexible SF films possessing silk I rather than silk II structure. Films were prepared by casting SF solution possessing SF nanoparticles induced by two different methods: (1) pre‐prepared SF nanoparticles by ethanol were added into SF solution; (2) nanoparticles were induced in SF solution via autoclaving. These films were compared with each other and with water‐annealed and methonol‐annealed films for their secondary structure and physical properties. The as‐prepared films were primarily composed of silk I structure, as validated by x‐ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. However, the water‐annealed and methanol‐annealed films exhibited silk II structure. The films prepared by induction of nanoparticles were water‐stable, transparent, mechanically strong (tensile strength as high as 5.14 ± 1.43 MPa), exhibited tunable degradation and sustained drug release properties. As for mechanical properties, these films displayed 4 times and 12 times improved mechanical ductility as compared with water‐annealed and methanol‐annealed films, respectively. Moreover, these films exhibited fast enzymatic degradation as compared with their counterparts with silk II structure. These films hold great promise as a biomaterial for tissue engineering applications especially where flexibility and fast degradation of scaffold are important.

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Fabrication of protease XIV-loaded microspheres for cell spreading in silk fibroin hydrogels

Cited by 6 publications

References 35 publications

Aggressive strategies for regenerating intervertebral discs: stimulus-responsive composite hydrogels from single to multiscale delivery systems

Aggressive strategies for regenerating intervertebral discs: stimulus-responsive composite hydrogels from single to multiscale delivery systems

Conformational Transition‐Driven Self‐Folding Hydrogel Based on Silk Fibroin and Gelatin for Tissue Engineering Applications

Incorporation of nanoparticles in silk fibroin solution: Toward water‐stable silk fibroin films possessing silk I structure

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