Fabrication of porous three‐dimensional fibroin structures through a freezing process

Kolahreez, Davood; Morshed, Mohammad

doi:10.1002/app.46537

Cited by 9 publications

(2 citation statements)

References 73 publications

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“…The mechanical properties (experimental and predicted) did not, however, correlate with the microstructure of the sponges (Panda et al, 2018). Kolahreez and Morshed concluded similarly (Kolahreez and Morshed, 2018). The new commonality with those studies is the lack of accurate 3D structures.…”

Section: Introductionmentioning

confidence: 90%

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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Three-dimensional scaffolds play an essential role in tissue engineering. Although essential, the tunability of the 3D scaffolds mechanical and transport properties remains a challenge. In this work, we present new approaches to advance the field. First, we applied our progressive pH acidification to mimic the natural silk gelation process before ice-templating (−20 and −80 • C); second, we fitted the mechanical properties using a connectivity model; third, we fitted the scaffolds mechanical relaxation to understand the transport properties; and fourth we used micro-CT to correlate the process parameters to the scaffolds' performances. Our results suggested that the free shrinkage of the scaffolds determined their final properties. We found, however, that the porosity (above 90%) was anisotropic, similarly the tortuosity (between 1 and 1.3). We identified two major pore dimensions, the first one between 10 and 20 µm, and the second between 50 and 130 µm. Mechanically, our model suggested that the bulk modulus captured the elastic contribution and was controlled predominantly by the silk concentration. We tentatively associated the fractional modulus 1 to the collapse of the larger pores structures and was controlled mostly by the process temperature. We assigned the slow relaxation to the transport of fluid in the silk sponge scaffolds; and the fast relaxation with a viscoelastic relaxation. The silk concentration and process temperatures did not influence the latter. Overall, our use of the tomography, mechanical test, and detailed statistical analysis provides inroads into the interplay between process parameters (silk concentration and process temperature) and the multiple responses of the silk sponge scaffolds. The development of a new mechanical fitting for the compression test helped capture simply the different failure modes in the sponge scaffolds as well as correlating those events to relaxation and eventually transport properties.

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Section: Introductionmentioning

confidence: 90%

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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“…Similarly, freezing the protein solution [9,16,[20][21][22] produces a lower energy state for the water (i.e., as ice), thereby enticing it to leave the hydration shell. This example is particularly instructive, as it is the increased entropy of liquid water overcoming the stronger enthalpic component of ice crystals that defines its melting point.…”

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confidence: 99%

Seeking Solvation: Exploring the Role of Protein Hydration in Silk Gelation

Laity

Holland

2022

Molecules

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The mechanism by which arthropods (e.g., spiders and many insects) can produce silk fibres from an aqueous protein (fibroin) solution has remained elusive, despite much scientific investigation. In this work, we used several techniques to explore the role of a hydration shell bound to the fibroin in native silk feedstock (NSF) from Bombyx mori silkworms. Small angle X-ray and dynamic light scattering (SAXS and DLS) revealed a coil size (radius of gyration or hydrodynamic radius) around 12 nm, providing considerable scope for hydration. Aggregation in dilute aqueous solution was observed above 65 °C, matching the gelation temperature of more concentrated solutions and suggesting that the strength of interaction with the solvent (i.e., water) was the dominant factor. Infrared (IR) spectroscopy indicated decreasing hydration as the temperature was raised, with similar changes in hydration following gelation by freezing or heating. It was found that the solubility of fibroin in water or aqueous salt solutions could be described well by a relatively simple thermodynamic model for the stability of the protein hydration shell, which suggests that the affected water is enthalpically favoured but entropically penalised, due to its reduced (vibrational or translational) dynamics. Moreover, while the majority of this investigation used fibroin from B. mori, comparisons with published work on silk proteins from other silkworms and spiders, globular proteins and peptide model systems suggest that our findings may be of much wider significance.

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Modulation of Biophysical Cues in Nature Inspired Patterning of Porous Silk Fibroin Scaffold for Replenishable Controlled Drug Delivery

Mukhopadhyay

Gope

Choudhury

et al. 2023

Macromolecular Bioscience

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While a sticking plasteris enough for healing of most of the minor cuts they may get routinely, critical situations like surgical, gunshot, accidental or diabetic wounds;lacarations and other cutaneous deep cuts may require implants and simultaneous medications for healing. From the biophysical standpoint, an internal force‐based physical surface stimulusis crucial for cellular sensing during wound repair. In this paper, the authors report the fabrication of a porous, biomimmetically patterned silk fibroin scaffold loaded with ampicillin, which exhibits controlled release of the drug along with possible replenishment of the same. In vitro swelling study reveals that the scaffolds with hierarchical surface patterns exhibit lower swelling and degradation than other types of scaffolds. The scaffolds, that show remarkable broad–spectrum antibacterial efficacy, exhibit Korsemeyer–Peppas model for the ampicillin release patterns due to the structural hydrophobicity imparted by the patterns. Four distinct cell‐matrix adhesion regimes are investigated for the fibroblasts to eventually form cell sheets all over the hierarchical surface structures. 4',6–diamidino–2–phenylindole (DAPI) and Fluorescein Diacetate (FDA) fluorescent staining clearly demonstrate the superiority of patterned surface over its other variants. A comparative immunofluorescence study among collagen I, vinculin, and vimentin expressions substantiated the patterned surface to be superior to others.

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Fabrication of porous three‐dimensional fibroin structures through a freezing process

Cited by 9 publications

References 73 publications

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

Seeking Solvation: Exploring the Role of Protein Hydration in Silk Gelation

Modulation of Biophysical Cues in Nature Inspired Patterning of Porous Silk Fibroin Scaffold for Replenishable Controlled Drug Delivery

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