Fabrication of polycaprolactone/chitosan/hydroxyapatite structure to improve the mechanical behavior of the hydrogel‐based scaffolds for bone tissue engineering: Biscaffold approach

Salami, S. Jedari; Soleimanimehr, Hamid; Maghsoudpour, Adel; Haghighi, Shahram Etemadi

doi:10.1002/pc.27428

Cited by 9 publications

(3 citation statements)

References 32 publications

(44 reference statements)

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“…The size of the scaffolds is a key factor that directly affects cell migration and viability. Increasing the size of the scaffold could decrease the cell count in the central areas 7 . Furthermore, in nonvascularized scaffolds, the gas interchanges and delivery of nutrients are very difficult and cause major cell death in the central zones of the scaffolds 8 .…”

Section: Introductionmentioning

confidence: 99%

“…Increasing the size of the scaffold could decrease the cell count in the central areas. 7 Furthermore, in nonvascularized scaffolds, the gas interchanges and delivery of nutrients are very difficult and cause major cell death in the central zones of the scaffolds. 8 In segmental tissue engineering, small-size scaffolds are considered as filler blocks, which can have pre-cell seeding to increase bone healing rapidity.…”

Section: Introductionmentioning

confidence: 99%

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In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

Khodabakhshi,

Soleimanimehr,

Etemadi Haghighi

et al. 2023

Polymer Engineering & Sci

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Segmental bone tissue engineering is a highly effective approach for the repair of large bone defects. In this article, 3D printing was used to fabricate the polycaprolactone (PCL)/NaCl‐based cylindrical structures. The effects of the structure's thickness (L) and NaCl concentration on the mechanical properties, degradability, swelling behavior, porosity, and cytotoxicity of the samples were investigated. Response surface methodology was employed to study the mechanical behavior using the central composite design (CCD). Results showed that increasing the NaCl concentration up to 10% wt significantly improved the degradability, swelling, and hydrophilicity of the structures. It was also indicated that the maximum stiffness of the guide structures under vertical loading was almost five times larger than the horizontal loading direction, but the samples showed greater compressive strength and elongation under horizontal compressive loading. Results indicated that the mechanical properties of the structures were more dependent on the structure thickness so the thicker structures with an 800 μm thickness had better mechanical properties in both vertical and horizontal loading. Cytotoxicity assay also approved the nontoxic effect of the PCL structures on the MC3T3 osteoblast cell line. Based on the results, the PCL‐based structures were a suitable candidate to act as a guide for segmental bone tissue engineering.Highlights A 3D‐printed conduit was manufactured to guide bone reconstruction Response surface methodology (RSM) using central composite design (CCD) was employed NaCl concentration and the diameter of strands were the main parameters This 3D‐printed guiding approach is useful for segmental bone tissue engineering

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

Khodabakhshi,

Soleimanimehr,

Etemadi Haghighi

et al. 2023

Polymer Engineering & Sci

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“…3 The synthesis of such polymeric networks using various combinations of polymers such as poly(acrylic acid)/linseed mucilage/chitosan, 4 carboxylated xanthan-carboxylated guar gum, 5 carbapol and poly(acrylamide)-functionalized Azadirachta indica gum, 6 cellulose acetate-poly(acrylic) acid, 7 sodium alginate/chitosan, 8 and polyacrylamide/chitosan/polypyrrole 9 is reported in the literature for controlled drug release applications. Various polysaccharide hydrogels, including but not limited to gellan gum, 10,11 chitosan, 12,13 alginate, 14 hyaluronic acid, 15 and carboxymethyl cellulose 16,17 were also developed for tissue engineering applications. These structures in multi-particulate forms have piqued the interest for controlled delivery of therapeutic molecules, 18 due to various therapeutic advantages.…”

Section: Introductionmentioning

confidence: 99%

Inorganic metal salts as additives in the design of cellulose/arabinogalactan hydrogels for prolonged delivery of simvastatin in simulated bio‐fluids

Agrawal,

Yadav,

Patel

et al. 2023

Vinyl Additive Technology

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In this study, carboxymethylcellulose and arabinogalactan were used to create an interpenetrating network (IPN) hydrogel system in the presence of AlCl3 alone or in combination with ZnSO4 salts. Ionotropic gelation caused the polymer sol droplets to transform into hydrogel particles in the presence of salts. The impact of salts on hydrogel properties was evaluated in terms of morphology, drug entrapment efficiency, swelling, and drug release kinetics. The cellulose IPN hydrogel particles containing 50% arabinogalactan looked spherical with evidence of surface folding when treated with AlCl3. Surface folding was reduced by an additional treatment with ZnSO4. Following treatment of the IPN particles with dual salts, a maximum drug entrapment efficiency of 88.77% was obtained. Surface erosion, as seen with aluminum‐IPN hydrogel particles, was minimized with the use of mixed salts as gelation medium. Furthermore, the use of mixed salts allowed the hydrogel particles to swell and consistently release simvastatin in simulated gastrointestinal fluids for up to 9 h. Thermal and x‐ray analyses revealed that the crystallinity of the drug reduced considerably after entrapment in the IPN hydrogel matrix. The infrared spectra analysis did not indicate any evidence of drug polymer interaction. The release of drug from the IPN hydrogel particles followed non‐Fickian diffusion mechanism. The dual metallic salts were found to be effective in creating physically stable cellulose‐arabinogalactan IPN hydrogel particles for sustained release of simvastatin in a varying pH environment of gastrointestinal tract.Highlights Dual inorganic salts allowed synthesis of cellulose/arabinogalactan hydrogel Additional use of ZnSO4 improved surface morphology of hydrogel particles Compatible environment of hydrogels allowed more than 88% drug entrapment Concentration of neutral arabinogalactan was crucial in dictating drug release Mixed salts controlled swelling and release of simvastatin from hydrogels

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Nanoscale hydroxyapatite/chitosan polymer coatings: Synthesis and characterization

Saeedi,

Montazeri

2023

Polymer Composites

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The present work involves the coating synthesis of nano‐hydroxyapatite/chitosan (nHA/CS) on a Mg‐2 wt% Zn scaffold with the pulse electrodeposition (PED) technique. In practice, to render coatings more corrosion‐resistant and biocompatibility in simulated body fluids, the impact of PED factors, such as mean current density (j), a duty cycle, and coating duration (t), was investigated. Further, Fourier transform infrared spectroscopy, scanning electron microscope, x‐ray diffraction, energy‐dispersion spectrometer, and water contact angle (WCA) were studied to characterize coatings. The corrosion resistance of coatings was investigated by impedance evaluations and potentiodynamic polarization. The results obtained from analyses revealed that the optimum conditions for synthesizing the nHA/CS coating were achieved when applying j = 20 mA/cm2, a duty cycle = 0.4, and a duration of 60 minutes. The coatings' microstructure and Ca/P ratio were influenced by PED factors, which included nano spherical‐shaped and 1.65. Furthermore, the corrosion rate was decreased from 19.603 to 1.492 mm yr−1 after being modified by optimum coating. Moreover, the high hydrophilic properties are assigned to this coating with a WCA of 29.9 compared to other coatings and the Mg scaffold. The biological properties of the optimum coating were assessed through in‐vitro experiments involving MG63 cells, which included the evaluations of cell adhesion and cytotoxicity. The results obtained from the corrosion and in‐vitro experiments indicate that the nanocomposite coating, upon optimization, holds potential as a scaffold for bone tissue engineering.Highlights Using Mg‐2 wt% Zn scaffold as a substrate for nanocomposite coatings. Pulse electrodeposition method for the synthesis of nano‐hydroxyapatite/chitosan (nHA/CS) coatings. Pulse electrodeposition (PED) method to modify morphology, corrosion resistance, and biocompatibility. Optimum PED parameters: j = 20 mA/cm2, duty cycle = 0.4, and t = 60 min.

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Fabrication of polycaprolactone/chitosan/hydroxyapatite structure to improve the mechanical behavior of the hydrogel‐based scaffolds for bone tissue engineering: Biscaffold approach

Cited by 9 publications

References 32 publications

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

Inorganic metal salts as additives in the design of cellulose/arabinogalactan hydrogels for prolonged delivery of simvastatin in simulated bio‐fluids

Nanoscale hydroxyapatite/chitosan polymer coatings: Synthesis and characterization

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