Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells

Abstract

Recently, bio-based electrospun nanofiber mats (ENMs) have gained substantial attention for preparing polymer-based biomaterials intended for use in cell culture. Herein, we prepared poly(ethylene-glycol 1,4-Cyclohexane dimethylene-isosorbide-terephthalate) (PEICT) ENMs using the electrospinning technique. Cell adhesion and cell viability of PEICT ENMs were checked by fibroblast cell culture. Field emission electron microscope (FE-SEM) image showed a randomly interconnected fiber network, smooth morphology, and cell adhesion on PEICT ENM. Fibroblasts were cultured in an adopted cell culturing environment on the surface of PEICT ENMs to confirm their biocompatibility and cell viability. Additionally, the chemical structure of PEICT ENM was checked under Fourier-transform infrared (FTIR) spectroscopy and the results were supported by -ray photoelectron (XPS) spectroscopy. The water contact angle (WCA) test showed the hydrophobic behavior of PEICT ENMs in parallel to good fibroblast cell adhesion. Hence, the results confirmed that PEICT ENMs can be potentially utilized as a biomaterial.

Preparation of a Cage-Type Polyglycolic Acid/Collagen Nanofiber Blend with Improved Surface Wettability and Handling Properties for Potential Biomedical Applications

“…Raman spectrum also confirmed the presence of C=O, N-C=O and increased CH 2 at 1781 cm −1 , 1658 cm −1 and 1425 cm −1 , respectively. As per previous reports, increased number of CH 2 groups on polymeric fiber's surfaces results in increased cell adhesion properties, which indicates the potential for cell culture applications of this optimized cage-type blend with 5% SuPMA content [59].…”

Electrospun Composite Nanofibers for Functional Applications

“…It was found that the PEICT nanofibers possessed excellent biocompatibility. Hence, the results confirmed that PEICT ENMs can potentially be utilized as a biomaterial [ 29 ]. Silver nanoclusters are also considered excellent antibacterial agents; another study in this Special Issue synthesized novel sericin-encapsulated silver nanoclusters (sericin-AgNCs) through a green synthesis route.…”

Electrospun Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)/Olive Leaf Extract Fiber Mesh as Prospective Bio-Based Scaffold for Wound Healing

“…In addition, keratinocytes covered the scaffold surface through cellular layers, which is indicative of epithelization capacity. We have recently disclosed the immunomodulatory activity of other PHA/OLE fibers in vitro using human dermal keratinocytes occurring via the downregulation of the inflammatory pattern, thus proving the potential for skin re-epithelization and regeneration, which are secondary to inflammation resolution [ 45 ].…”