Fabrication of NaYF4:Yb,Er Nanoprobes for Cell Imaging Directly by Using the Method of Hydrion Rivalry Aided by Ultrasonic

Abstract

A novel method of fabricating water-soluble bio-probes with ultra-small size such as NaYF4:Yb,Er (18 nm), NaGdF4:Yb,Er (8 nm), CaF2:Yb,Er (10 nm), PbS (7 nm), and ZnS (12 nm) has been developed to provide for the solubility switch of nanoparticles from oil-soluble to water-soluble in terms of hydrion rivalry aided by ultrasonic. Using NaYF4:Yb,Er (18 nm) as an example, we evaluate the properties of as-prepared water-soluble nanoparticles (NPs) by using thermogravimetric analyses (TGA), Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ) testing, and 1H nuclear magnetic resonance (1HNMR). The measured ζ value shows that the newly prepared hydrophilic NaYF4:Yb,Er NPs are the positively charged particles. Acting as reactive electrophilic moiety, the freshly prepared hydrophilic NaYF4:Yb,Er NPs have carried out the coupling with amino acids and fluorescence labeling and imaging of HeLa cells directly. Experiments indicate that the method of hydrion rivalry aided by ultrasonic provides a simple and novel opportunity to transform hydrophobic NPs into hydrophilic NPs with good reactivity, which can be imaging some specific biological targets directly.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1651-y) contains supplementary material, which is available to authorized users.

Self-assembled pearl-necklace patterned upconverting nanocrystals with highly efficient blue and ultraviolet emission: femtosecond laser based upconversion properties

“…S5 † which conrms the presence of various functional groups including N-H bending vibration at 1558 cm À1 and other oleic acid-capped groups at 2921 cm À1 , 2850 cm À1 appearing with highest intensity attached with the UCN-PNs. 40,41 However, the present analysis gives us a clear indication of aand b-phase formation within UCN-PNs crystals and is further supported by the TEM and XRD results (Fig. 1(f) and S1 †).…”

Fabrication and characterization of up-converting β-NaYF4:Er3+,Yb3+@NaYF4 core–shell nanoparticles for temperature sensing applications

“…The broad bands at about 1635 cm −1 and 1122 cm −1 have been assigned www.nature.com/scientificreports/ www.nature.com/scientificreports/ to the presence of asymmetric and symmetric C = O stretching vibration of carboxylic acid salts on the surface of β-NaYF 4 :Er 3+ ,Yb 3+ CNP and β-NaYF 4 :Er 3+ ,Yb 3+ @NaYF 4 CSNP 31 and the peaks at 547 cm −1 and 515 cm −1 might be a result of a presence of the RE 3+ -F stretching vibrations. Exceptionally, a strong band at 1430 cm −1 related to O-H bending vibration of carboxyl group was observed only in the β-NaYF 4 :Er 3+ ,Yb 3+ @NaYF 4 CSNP 17,30 . Therefore, the presence of carboxylic acid groups in the synthesized β-NaYF 4 :Er 3+ ,Yb 3+ @NaYF 4 CSNP is suspected 30 .…”

“…In recent years, the upconversion luminescence of rare-earth ions (RE 3+ ) doped fluoride nanomaterials has received much attention due to their potential applications in various fields such as bioimaging, biolabeling, photodynamic therapy, as drug carriers, optical temperature sensors, etc. [11][12][13][14][15][16][17][18] . In the case of the optical temperature sensing applications, researchers focused especially on the sodium yttrium fluoride nanoparticles doped or co-doped with rare earth ions (NaYF 4 :RE 3+ nanoparticles) [19][20][21][22][23][24] , among which the NaYF 4 :Er 3+ , Yb 3+ nanoparticles were regarded as one of the most efficient upconversion materials for temperature sensing due to their unique luminescence properties and the large energy gap between two thermally coupled 2 H 11/2 and 4 S 3/2 energy levels [25][26][27] .…”

Design of Hydroxyapatite-Based Multifunctional Nanoparticles for Cell Labelling and Cell Growth Inhibition

“…The cell-labeling techniques have the potential to be improved by the development of cytocompatible photoluminescence probes [13,14]. For example, lanthanide (Ln) iondoped nanomaterials provide several advantages such as lower cytotoxicity, photostability and sharp luminescence bands over the conventional cell-labeling nanomaterials including organic molecules and semiconductor quantum dots [15,16]. From this viewpoint, we describe the importance of photofunctionalization of hydroxyapatite, which is realized by designing the inorganic-organic nanohybrid structures.…”