Fabrication of multifunctional monometallic nanohybrids for reactive oxygen species-mediated cell apoptosis and enhanced fluorescence cell imaging

Hu, Shengqiang; Ye, Baoyu; Tang, Hailin; Wu, Fan; Yi, Xinyao; Yi, Tiantian; Wu, Daohong; Wu, Ling; Wang, Jianxiu

doi:10.1039/c7tb02745a

Cited by 15 publications

(9 citation statements)

References 62 publications

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“…Note that before laser irradiation, the free AuNSs were washed away. For this assay, a live/dead stain was utilized by employing calcein-AM and propidium iodide (PI) dyes, where live and dead cells were stained with green and red, respectively. − From the overlay image (Figure A), exposure of MCF-7 cells to 808 nm laser irradiation or the AuNSs alone did not kill the MCF-7 cells. Laser irradiation of the S2.2-AuNSs killed most of the cells evidenced by the presence of few green fluorescent cells.…”

Section: Resultsmentioning

confidence: 99%

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Tong

Wang

et al. 2019

Anal. Chem.

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Pressure-based signal transduction has attracted recent and extensive attention due to its high sensitivity and simplicity. The most popular way to generate gas pressure relies on catalyst-mediated decomposition of H2O2. Despite its high sensitivity, this method lacks spatial and temporal control of the reaction, and may suffer from variations due to the dead time of mixing. In this work, we report a new reaction using near-infrared (NIR) light to heat hollow porous gold nanospheres (AuNSs) for thermal decomposition of NH4HCO3. Comparisons were made on these two systems especially on controlled pressure production. As an example of application, our light-controlled system was used for the detection of MCF-7 cancer cells by attaching the S2.2 aptamer on the AuNSs. The detection limit was as low as 2 cells/mL. Meanwhile, the heat produced by the AuNSs was used to induce localized hyperthermia at the surface of the cancer cells. This interesting theranostic system provides new insights into pressure-based sensing and may inspire new analytical applications.

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Section: Resultsmentioning

confidence: 99%

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Tong

Wang

et al. 2019

Anal. Chem.

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“…The integration of Cu and Au not only solved the problem of functionalization of Cu nanomaterial, but also greatly improved the photostability of the Au nanomaterials, while the introduction of aptamers enabled the efficient localization and accumulation of the nanoprobes in cancer cells and tissues, resulting in the simultaneous fluorescent molecular imaging and NIR PTT of the target cancer. More recently, Hu et al reported another fluorescent monometallic Ag nanohybrids (Ag NHs) synthesized via polycytosine‐mediated Ag NC biomineralization on Ag NPs. The aptamer‐functionalized Ag NHs served as an efficient therapeutic agent and can induce reactive oxygen species generation leading apoptosis once it is delivered into target cells.…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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“…The functionalization of the nanozymes is thus preferred. Owing to the sequence programmability and specific binding to the target molecules, DNA serves as an ideal alternative for nanomaterial functionalization. − Without any modification, DNA could be assembled on noble metal nanoparticles (e.g., gold) via metal–base interaction, graphene oxide through π–π stacking, , and metal oxides with the aid of a phosphate backbone . The functionalization of nanomaterials could also be performed via covalent modification using thiolated, aminated, and phosphated DNA .…”

Section: Introductionmentioning

confidence: 99%

Bifunctional Diblock DNA-Mediated Synthesis of Nanoflower-Shaped Photothermal Nanozymes for a Highly Sensitive Colorimetric Assay of Cancer Cells

Wei

et al. 2021

ACS Appl. Mater. Interfaces

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The activity of a nanozyme is closely related to its surface area-to-volume ratio and the surrounding temperature. To acquire highly active nanozymes, one-pot metallization-like synthesis of novel nanoflower-shaped photothermal nanostructures was conducted using polyadenine-containing diblock DNA as the scaffold. The nanoflower-shaped structures with a high surface area-to-volume ratio and photothermal performance exhibited excellent peroxidase-mimicking activity, and the biorecognition capability was retained by the capping agent of diblock DNA. The functionalized nanostructures were used for a proof-of-concept colorimetric assay of cancer cells in vitro. Upon incorporation of 808 nm laser irradiation, high sensitivity and selectivity for the cancer cell assay were achieved with the lowest detection level of 10 cells/mL. Relative to spherical gold nanostructures, the nanoflower-shaped photothermal nanozyme exhibited higher assay sensitivity, paving the way for the construction of nanozyme-based colorimetric sensors for point-of-care testing.

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Fabrication of multifunctional monometallic nanohybrids for reactive oxygen species-mediated cell apoptosis and enhanced fluorescence cell imaging

Abstract: Multifunctional monometallic nanohybrids were fabricated for ROS-mediated cell apoptosis and enhanced fluorescence cell imaging.

Cited by 15 publications

References 62 publications

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Bifunctional Diblock DNA-Mediated Synthesis of Nanoflower-Shaped Photothermal Nanozymes for a Highly Sensitive Colorimetric Assay of Cancer Cells

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