Fabrication of mulberry leaf extract (MLE)- and tasar pupal oil (TPO)-loaded silk fibroin (SF) hydrogels and their antimicrobial properties

R, Venkatesh Kumar; Srivastava, Devika; Kumar, Devarsh; Verma, Anshika; Verma, Darshika; Fatima, Eram; Chaudhary, Priyanka; Kumar, Umesh; Kumar, Dinesh

doi:10.1007/s13205-022-03443-5

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…The microphotographs and values of the elemental analyses of tested mulberry lattices were recorded using a scanning electron microscope (JEOL, JSM 6490 LV, Japan) built with an equipped energy-dispersive X-ray analyzer (EDX)(EDS 133, EV Dry Detector (INCA x-act) of the OXFORD instruments, U.K.). For this procedure, selected lattices were coated on aluminum stubs, and the instrument was operated at the voltage of 15 kV followed by examining them under 1000 and 3500× magnifications and recording the elemental trends …”

Section: Materials and Methodsmentioning

confidence: 99%

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Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices

Kumar R,

Gosipatala,

Kumar

et al. 2023

ACS Omega

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In order to find the most advantageous bioactive compounds from mulberry latex for drug development in the near future, this study was conducted to characterize and evaluate antioxidant and antimicrobial properties from four different mulberry lattices (BR-2, S-1, AR-14, and S-146). The characterization of the lattices was performed by scanning electron microscopy with energy-dispersive X-ray spectroscopy, gas chromatography coupled to mass spectroscopy, and Fourier transform infrared spectroscopy. Further, screenings of the antioxidant and antimicrobial potential of selected lattices were performed in vitro using 2,2-diphenyl-1-picrylhydrazyl assay and agar well diffusion methods, respectively. Interestingly, the outcome of the current study revealed that tested mulberry lattices contain a considerable amount of bioactive phytoconstituents, particularly antimicrobial and antioxidant compounds, as revealed by chromatographic analysis. BR-2 latex was found to have significant antioxidant activity (75%) followed by S-146 (64.6%) and AR-14 (52.9%). The maximum antimicrobial activity was found in BR-2 latex compared to other tested latex varieties. The results of this investigation showed that mulberry latex from the BR-2 type may successfully control both bacterial and fungal infections, with the added benefit of having enhanced antioxidant capabilities.

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Section: Materials and Methodsmentioning

confidence: 99%

“…For this procedure, selected lattices were coated on aluminum stubs, and the instrument was operated at the voltage of 15 kV followed by examining them under 1000 and 3500× magnifications and recording the elemental trends. 19 2.4. Gas Chromatography−Mass Spectroscopy (GC− MS) Investigation.…”

Section: Scanning Electronmentioning

confidence: 99%

Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices

Kumar R,

Gosipatala,

Kumar

et al. 2023

ACS Omega

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“…Much work has not been done on tropical Tasar silkworm (Antherea mylitta) pupae when compared to mulberry, eri, and muga pupae. In our preliminary study, we reported that tasar pupal oil is a rich source of alpha-linolenic acid (ALA) (37%), 19 which is an omega-3 essential fatty acid; therefore, It may prove to be a more beneficial substitute for edible oil. For this reason, in the current effort, we have extracted and characterised tasar pupae oil for commercial and therapeutic uses.…”

Section: Introductionmentioning

confidence: 99%

Tasar silkworm pupal oil: An excellent source of edible oil for industrial and therapeutic applications

Srivastava,

Singh,

Ramappa

2023

J. Drug Delivery Ther.

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Polyunsaturated fatty acids are widely recognized due to their remedial properties. In our previous studies, we reported that Tasar silkworm pupal oil (TSPO) is rich in polyunsaturated fatty acids, particularly alpha-linolenic acid (ALA). Further we also extracted ALA from TSPO and reported its significant anticancer activity. In the present study, extraction of TSPO and physico-chemical characterizations including saponification, acid, peroxide, iodine pH values, and colour determination were carried out to investigate the edible properties of TSPO for commercialization as a therapeutically active functional food. Further, the anti-diabetic activity of TSPO were analyzed by α-amylase inhibition assay. The saponification (154.75 ± 0.581mg KOH/g), acid (6.24 ± 0.421 mg KOH/g), peroxide (16.20 ± 0.265 meq/kg), iodine (40.33 ± 0.577g/100 ml), pH (6.17), and colour (14 Y+1.2 R) values were within the permissible range for commercialization. In addition to this, the results of the α-amylase inhibition assay showed that TSPO exhibited an IC50 value of 207339 µg/ml. The results may facilitate the use of TSPO oil in therapeutic and functional food applications Keywords: Characterization; Commercialization; Tasar pupal oil; Silkworm pupae.

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“…However, both mulberry and non-mulberry silkworm pupal oils have been identified as new sources of ALA in recent days 4,5,6 . Among the silkworm pupal oils, Antherea mylitta (Tasar silkworm) (Lepidoptera: Saturniidae) pupal oil has emerged as a rich source of ALA, as it revealed 37% of ALA in it 7 .…”

Section: Introductionmentioning

confidence: 99%

Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

Srivastava

Kumar

2023

J. Drug Delivery Ther.

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Previously, we reported that tasar silkworm pupal oil (TPO) is a rich source of alpha-linolenic acid (ALA) (37%). In this study, we have used three chromatographic techniques, namely preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), and gas chromatography flame ionization detection (GC-FID), to purify, determine, and quantify alpha-linolenic acid (ALA), respectively, from TPO. Additionally, to determine the potential of the purified ALA as an anticancer drug, the sulforhodamine B (SRB) assay was utilised for in vitro investigation. Results revealed that ALA was successfully purified in a fraction of TPO by preparatory HPLC. It was discovered that only ALA was present in the purified fraction of ALA when it underwent GC-MS analysis, further confirming its purity. In addition, the quantification analysis using GC-FID showed that the concentration of ALA was 11760 ppm. Furthermore, anticancer analysis revealed purified ALA significantly inhibited the growth of a colon cancer cell line, (COLO-205) with a GI 50 ≤20. This is the initial investigation into isolating and purifying ALA from Tasar pupal oil. Keywords: Alpha-linolenic acid; GC-FID; HPLC; Omega-3Preparative, Tasar pupal oil.

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Fabrication of mulberry leaf extract (MLE)- and tasar pupal oil (TPO)-loaded silk fibroin (SF) hydrogels and their antimicrobial properties

Cited by 5 publications

References 39 publications

Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices

Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices

Tasar silkworm pupal oil: An excellent source of edible oil for industrial and therapeutic applications

Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

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