Fabrication of micropatterns of nanoarrays on a polymeric gel surface

Abstract

Micro-nano patterns of gold on the surface of poly(ethylene glycol) (PEG) hydrogels were prepared. The approach combines the technique of conventional photolithography (a top-down method for micropatterns), block copolymer micelle nanolithography (a bottom-up method for gold nanopatterns), and a linker-assistant technique to transfer a pattern on a hard surface to a polymeric surface. Hybrid micro-nano patterns on hydrogels were characterized using scanning electron microscopy, atomic force microscopy and X-ray photoelectron spectroscopy. The patterned Au nanoparticles were further modified by a peptide containing arginine-glycine-aspatate (RGD). The cell-adhesion contrast of the patterned hydrogel surface was confirmed by preliminary cell experiments.

Large Array of Sub‐10‐nm Single‐Grain Au Nanodots for use in Nanotechnology

“…Extensive and perfectly ordered arrays of close‐to‐identical nanoparticles (NPs) have attracted much attention for their numerous potential applications in several nanotechnology fields, including materials science,1, 2 electronics,3, 4 biology,5 and information technology 6, 7. Gold nanoparticles a few nanometers across and distributed in a liquid solution are already commercially available.…”

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

“…The resulting gold patterns on PEG hydrogel were further functionalized with cell adhesive biomolecules. Using the same strategy micro and nanopatterns on hydrogels were prepared and used for cellular patterning and the controlling of cell adhesion by the density and distance of bioactive molecules on those gold patterns . In another approach, surface chemistry and topographical contrast were used to influence adhesion, migration, differentiation, and guidance of neurons.…”

Reconstructing the Differentiation Niche of Embryonic Stem Cells Using Biomaterials

“…2D patterned cues have been fabricated in the nanoscale regime, and have demonstrated a profound effect on cellular response, including adhesion,134, 135 motility,136 and stem cell function 137. Although there have been recent advances in nanopatterning techniques and complexities,138, 139 these designs have yet to be utilized to study stem cell differentiation. Culturing human ES cells on fibronectin‐coated PDMS substrates with nanogratings enhanced such morphological changes to ES cells as elongation and alignment, demonstrating distinct cytoskeletal reorganization via contact guidance while also reducing ES cell proliferation 127…”