Fabrication of micro-cages and caged tumor spheroids for microfluidic chip-based assays

He, Yuqing; Huang, Boxin; Rofaani, Elrade; Hu, Jie; Liu, Yuanhui; Pitingolo, Gabriele; Wang, Li; Shi, Jian; Aimé, Carole; Chen, Yong

doi:10.1016/j.mee.2020.111256

Cited by 11 publications

(6 citation statements)

References 25 publications

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“…As a result, most of the current researches in the field elaborate protocols to grow cells in 3D constructs [112]. These techniques involve the seeding of cells on non-adherent substrates such as bovine serum albumin (BSA) [113,114], polyHEMA [95,115], agarose [65,116,117], or Pluronic F127 [118,119] or the use of EOC cell lines known to spontaneously aggregate in given conditions [46,120]. Alternatively, spheroids can be produced by using hydrogels [79,81,[121][122][123][124], hanging drop culture [125,126], or rotating wall culture [94,127,128] that have all proven their efficiency in the past ten years for the generation of reproducible and stable organoids [129,130].…”

Section: Spheroid Modelsmentioning

confidence: 99%

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

et al. 2023

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Ovarian cancer (OC) is a disease of major concern with a survival rate of about 40% at five years. This is attributed to the lack of visible and reliable symptoms during the onset of the disease, which leads over 80% of patients to be diagnosed at advanced stages. This implies that metastatic activity has advanced to the peritoneal cavity. It is associated with both genetic and phenotypic heterogeneity, which considerably increase the risks of relapse and reduce the survival rate. To understand ovarian cancer pathophysiology and strengthen the ability for drug screening, further development of relevant in vitro models that recapitulate the complexity of OC microenvironment and dynamics of OC cell population is required. In this line, the recent advances of tridimensional (3D) cell culture and microfluidics have allowed the development of highly innovative models that could bridge the gap between pathophysiology and mechanistic models for clinical research. This review first describes the pathophysiology of OC before detailing the engineering strategies developed to recapitulate those main biological features.

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Section: Spheroid Modelsmentioning

confidence: 99%

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

et al. 2023

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“…Thus, although the culture media flow at a constant rate from the outside region of the structures, the static environment created within the structures promotes cell fusion and the formation of spheroids . Although there are not as many literature examples as the hanging-drop method and multiwell-based approach, there are promising examples using microstructures, which can be practically applied using photolithographic and molding approaches. − ,, For example, LCC6 breast cancer cells encapsulated with alginate beads having a size of approximately 250 μm were trapped via a U-shaped microstructure for spheroid formation. The effect of doxorubicin on the produced spheroids was also studied on the same chip .…”

Section: Spheroid Engineering In Microfluidic Systemsmentioning

confidence: 99%

Spheroid Engineering in Microfluidic Devices

et al. 2023

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cell culture techniques are commonly employed to investigate biophysical and biochemical cellular responses. However, these culture methods, having monolayer cells, lack cell−cell and cell−extracellular matrix interactions, mimicking the cell microenvironment and multicellular organization. Three-dimensional (3D) cell culture methods enable equal transportation of nutrients, gas, and growth factors among cells and their microenvironment. Therefore, 3D cultures show similar cell proliferation, apoptosis, and differentiation properties to in vivo. A spheroid is defined as self-assembled 3D cell aggregates, and it closely mimics a cell microenvironment in vitro thanks to cell−cell/matrix interactions, which enables its use in several important applications in medical and clinical research. To fabricate a spheroid, conventional methods such as liquid overlay, hanging drop, and so forth are available. However, these labor-intensive methods result in low-throughput fabrication and uncontrollable spheroid sizes. On the other hand, microfluidic methods enable inexpensive and rapid fabrication of spheroids with high precision. Furthermore, fabricated spheroids can also be cultured in microfluidic devices for controllable cell perfusion, simulation of fluid shear effects, and mimicking of the microenvironment-like in vivo conditions. This review focuses on recent microfluidic spheroid fabrication techniques and also organ-on-a-chip applications of spheroids, which are used in different disease modeling and drug development studies.

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“…Two independent studies printed tumour spheroids seeded on tumour cell-specific microfluidic devices. A 3D printed cage device and another device with valve control for controlled delivery of nutrients and drugs are designed for future applications in drug screening (77,78).…”

Section: Tumour-on-chip Modelsmentioning

confidence: 99%

3D Printed Personalized Medicine for Cancer: Applications for Betterment of Diagnosis, Prognosis and Treatment

2021

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Cancer treatment is challenging due to the tumour heterogeneity that makes personalized medicine a suitable technique for providing better cancer treatment. Personalized medicine analyses patient-related factors like genetic make-up and lifestyle and designs treatments that offer the benefits of reduced side effects and efficient drug delivery. Personalized medicine aims to provide a holistic way for prevention, diagnosis and treatment. The customization desired in personalized medicine is produced accurately by 3D printing which is an established technique known for its precision. Different 3D printing techniques exhibit their capability in producing cancer-specific medications for breast, liver, thyroid and kidney tumours. Three-dimensional printing displays major influence on cancer modelling and studies using cancer models in treatment and diagnosis. Three-dimensional printed personalized tumour models like physical 3D models, bioprinted models and tumour-onchip models demonstrate better in vitro and in vivo correlation in drug screening, cancer metastasis and prognosis studies. Three-dimensional printing helps in cancer modelling; moreover, it has also changed the facet of cancer treatment. Improved treatment via custommade 3D printed devices, implants and dosage forms ensures the delivery of anticancer agents efficiently. This review covers recent applications of 3D printed personalized medicine in various cancer types and comments on the possible future directions like application of 4D printing and regularization of 3D printed personalized medicine in healthcare.Keywords personalized medicine • Tumour-on-chip • 3D bioprinting • 3D printing • Cancer modelling

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Fabrication of micro-cages and caged tumor spheroids for microfluidic chip-based assays

Cited by 11 publications

References 25 publications

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

Spheroid Engineering in Microfluidic Devices

3D Printed Personalized Medicine for Cancer: Applications for Betterment of Diagnosis, Prognosis and Treatment

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