Fabrication of metallic microchannel mold using X-ray LIGA for microfluidic applications

Phatthanakun, Rungrueang; Yunphuttha, Chumphol; Pantong, C.; Sriphung, C.; Chomnawang, Nimit; Viravathana, Pinsuda

doi:10.1109/ecticon.2013.6559467

Cited by 4 publications

(1 citation statement)

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“…The microwell layer was created by standard PDMS soft lithography using the nickel mold which was fabricated using X-ray LIGA technique 31 (Fig. 1(c)).…”

Section: Methodsmentioning

confidence: 99%

An electrostatic microwell–based biochip for phytoplanktonic cell trapping

et al. 2014

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A simple microwell-based microfluidic chip for microalgal cells trapping was fabricated. An electrostatic cell trapping mechanism, enabled by a positively charged glass surface, was used. The chip was capable of capturing multiple algal cell types. In the case of filamentous Spirulina platensis, we observed single filament occupancy of up to $30% available wells, as high as some previously proposed methods. Captured filaments were not of any preferential size, suggesting well randomized cell trapping. It was found that the electrostatic attraction did not affect the cell growth. Total replacement of liquid inside the wells could be achieved by pumping new solutions via the inlet, making single cell experiments in controlled chemical conditions possible. After the top layer of the chip was removed, cells in the wells could be simply transferred using a micropipette, turning the chip into a platform for strain selection. V C 2014 AIP Publishing LLC. [http://dx

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“…The microwell layer was created by standard PDMS soft lithography using the nickel mold which was fabricated using X-ray LIGA technique 31 (Fig. 1(c)).…”

Section: Methodsmentioning

confidence: 99%

An electrostatic microwell–based biochip for phytoplanktonic cell trapping

et al. 2014

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A facile method for generating polypyrrole microcapsules and their application in electrochemical sensing

et al. 2022

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A facile and rapid strategy to generate polypyrrole microcapsules is reported. The strategy is compatible with a vortex mixer and with a microfluidic chip for droplet generation, allowing a > 100-fold reduction in particle size. The sub-micron particle sizes obtained can also be tuned to some extent based on the chip geometry. The capsules can be kept stably in solution and can be transferred onto electrochemical devices. As an application example, we casted the polypyrrole capsules generated onto screen-printed electrodes, leading to a significant increase in their electroactive surface area and capacitance. The electrodes were further modified with glucose dehydrogenase (GDH) to fabricate glucose biosensors. The introduction of polypyrrole microcapsules increased the dynamic range of the glucose sensor to ca. 300% compared with that of the electrode without polypyrrole microcapsules. The resulting glucose sensor is operated at a constant applied potential of 0.20 V vs. Ag/AgCl (3 M KCl) in an air-equilibrated electrolyte. At this potential, the sensor showed a linear range from 1.0 to 9.0 mM glucose with a sensitivity of 3.23 µA cm−2 mM−1 (R2 = 0.993). The limit of detection obtained was 0.09 mM, and the reproducibility was 3.6%. The method allows generating polypyrrole microcapsules without surfactants or organic solvents and may enable new opportunities in the design of biosensors, electronic devices, and molecular delivery. Graphical abstract

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