Fabrication of homogeneously cross-linked, functional alginate microcapsules validated by NMR-, CLSM- and AFM-imaging

Zimmermann, Heiko; Hillgärtner, Markus; Manz, B.; Feilen, Peter J.; Brunnenmeier, Frank; Leinfelder, Ulrich; Weber, M.; Cramer, Hubert; Schneider, Stephan; Hendrich, Christian; Volke, F.; Zimmermann, U.

doi:10.1016/s0142-9612(02)00639-7

Cited by 86 publications

(62 citation statements)

References 24 publications

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“…Isolation of rat islets was performed according to the protocol previously described (17,18). Briefly, SD rats (Central Animal Facility, University of Mainz) age 8 weeks and weighing 280Ϫ330 g were used as islet donors.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, Omer et al (15) found that Ba 2ϩ Ϫcross-linked alginate microcapsules protect neonatal pancreatic cell clusters (NPCCs) when transplanted in the streptozotocininduced diabetic Balb/c mouse model for 20 weeks. We have recently shown that using highly purified and ultrahigh-iscosity alginates when cross-linked internally and externally with Ba (2) results in microcapsules of extremely high stability, particularly when proteins (e.g., human serum albumin [HSA]) are simultaneously incorporated (16,17). A recent study has shown that rat islets encapsulated in these novel microcapsules exhibit a wellpreserved insulin secretion in tissue culture over 3 weeks (16).…”

mentioning

confidence: 99%

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Long-Term Graft Function of Adult Rat and Human Islets Encapsulated in Novel Alginate-Based Microcapsules After Transplantation in Immunocompetent Diabetic Mice

Schneider¹,

Feilen²,

et al. 2005

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We describe the results of the first study to show that adult rat and human islets can be protected against xenogenic rejection in immunocompetent diabetic mice by encapsulating them in a novel alginate-based microcapsule system with no additional permselective membrane. Nonencapsulated islets lost function within 4 -8 days after being transplanted into diabetic Balb/c mice, whereas transplanted encapsulated adult rat or human islets resulted in normoglycemia for >7 months. When rat islet grafts were removed 10 and 36 weeks after transplantation, the mice became immediately hyperglycemic, thus demonstrating the efficacy of the encapsulated islets. The explanted capsules showed only a mild cellular reaction on their surface and a viability of >85%, and responded to a glucose stimulus with a 10-fold increase in insulin secretion. Furthermore, transplanted mice showed a slight decrease in the glucose clearance rate in response to intraperitoneal glucose tolerance tests 3-16 weeks after transplantation; after 16 weeks, the rate remained stable. Similar results were obtained for encapsulated human islets. Thus we provide the first evidence of successful transplantation of microencapsulated human islets. In conclusion, we have developed a novel microcapsule system that enables survival and function of adult rat and human islets in immunocompetent mice without immunosuppression for >7 months. Diabetes 54:687-693, 2005

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Long-Term Graft Function of Adult Rat and Human Islets Encapsulated in Novel Alginate-Based Microcapsules After Transplantation in Immunocompetent Diabetic Mice

Schneider¹,

Feilen²,

et al. 2005

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“…In case of a homogenous solid mass, where the entrapped cells are closely interacting with the biomaterials, the biomaterials should have good biocompatibility enabling cellular attachment and growth. Nevertheless, the commonly used encapsulating materials such as alginate and agarose have limited ability to support cell attachment growth and differentiation, resulting in low cell viability and growth [42,83,131]. In many cases, a biomaterial with better biocompatibility such as collagen must be supplemented for improvement in cell viability [10,42].…”

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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“…Many naturally derived biomaterials have been used for encapsulation, such as sodium alginate [9] and agarose [10]. However, these biomaterials have limited ability to support cell attachment, growth and differentiation, resulting in low cell viability and growth [11]. Carboxymethyl cellulose (CMC) is a water-soluble, biodegradable and biocompatible derivative of cellulose.…”

Section: Introductionmentioning

confidence: 99%

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

Ke¹,

Liu²,

Wang

et al. 2014

Express Polym. Lett.

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Abstract. Biocompatible and biodegradable carboxymethyl cellulose (CMC) has been modified with 4-hydroxybenzylamine (CMC-Ph) in order to prepare CMC-based microgels through the horseradish peroxidise/hydrogen peroxide enzymatic reaction. CMC-Ph was identified as a blend, and the amount of the grafted 4-hydroxybenzylamine per 100 units of CMC was between 17 and 23 according to the molecular weight of CMC. Through a special designed co-flowing microfluidic device, CMC-Ph microgels were prepared with the radius from 100 to 500 µm via adjusting the flow rates of the disperse phase and the continuous phase, respectively. The chondrocytic cell line ATDC5 was encapsulated in the CMC-Ph microgels. The cell-laden microgels were cultured for up to 40 days, illustrating the biocompatibility of CMC-Ph and the microfluidic approach through the enzymatic crosslinking reaction primarily. CMC-Ph showed a great promise to encapsulate the cells for further fabrication of the injectable scaffolds.

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Fabrication of homogeneously cross-linked, functional alginate microcapsules validated by NMR-, CLSM- and AFM-imaging

Cited by 86 publications

References 24 publications

Long-Term Graft Function of Adult Rat and Human Islets Encapsulated in Novel Alginate-Based Microcapsules After Transplantation in Immunocompetent Diabetic Mice

Long-Term Graft Function of Adult Rat and Human Islets Encapsulated in Novel Alginate-Based Microcapsules After Transplantation in Immunocompetent Diabetic Mice

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

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