Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization

Li, Shu; Sun, Yan; Shi, Qinghong

doi:10.1016/j.bej.2015.07.010

Cited by 17 publications

(2 citation statements)

References 43 publications

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“…Similar phenomena were observed in Figure b for FF‐D100‐DexD50, and FF‐DexD160 (IC, 154 ± 5 mmol/L), as well as FF‐D60‐DexD160 and FF‐DexD200 (IC, 212 ± 13 mmol/L). Particularly, the D e / D 0 value of FF‐D60‐DexD160 was as high as 2.52 ± 0.05, which is much higher than the reported polymer functionalized resins . The above results suggest that the surface ligand assisted the grafting ligands in facilitating protein uptake kinetics.…”

Section: Resultsmentioning

confidence: 71%

Surface DEAE groups facilitate protein transport on polymer chains in DEAE‐modified‐and‐DEAE‐dextran‐grafted resins

Gong

Bai

et al. 2016

AIChE Journal

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in Wiley Online Library (wileyonlinelibrary.com)Sepharose FF was modified with diethylaminoethyl-dextran (DEAE-dextran, DexD) and/or DEAE (D) to fabricate three types of ion exchangers FF-DexD (grafting-ligand resin), FF-D (surface-ligand resin), and FF-D-DexD (mixed-ligand resin), for protein adsorption equilibria and kinetics study. It was found that both adsorption capacity and uptake rate (effective diffusivity, D e ) were significantly enhanced by grafting DEAE-dextran. Notably, the D e values on FF-DexD and FF-D-DexD (D e /D 0 > 1.4) were six times greater than those on FF-D (D e /D 0 < 0.3). More importantly, the increase of surface-ligand density greatly enhanced uptake kinetics on FF-D-DexD. The results indicate that the surface ligands assisted the transport of bound proteins on polymer chains in the mixed-ligand resins. That is, surface ligands worked as "transfer stations" between two neighboring chains, resulting in enhanced transport of bound proteins on chains. The research thus disclosed the unique role of surface ligands in facilitating protein uptake kinetics onto polymergrafted ion-exchangers.The static adsorption equilibrium experiments of BSA on the resins were carried out in 20 mmol/L Tris-HCl (pH 8.0) as described earlier. 24 The adsorption density of protein (q) was calculated by mass balance with the free protein concentration in equilibrium (c), and the Langmuir model was used to describe the adsorption isotherms,The black lines represent agarose matrix, the blue curves represent dextran chains, the green lines represent the spacers, the red circles represent ion-exchange groups, and the pink and purple circles represent bound proteins. The dashed blue curves and faded circles represent the grafted polymer chains with adsorbed proteins after swing, and the dashed black boxes highlight the sites for the "relay"/delivery of proteins. The quantities of the dextran chains, spacers and the ion-exchange groups in this figure do not represent their real densities in the resins. In (a) it shows that the upper protein (pink) on the chain is delivered while the lower one (purple) is not. In (b) it shows that chain delivery occurs for the lower protein (purple), which was mediated by the surface DEAE ligands. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

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Section: Resultsmentioning

confidence: 71%

Surface DEAE groups facilitate protein transport on polymer chains in DEAE‐modified‐and‐DEAE‐dextran‐grafted resins

Gong

Bai

et al. 2016

AIChE Journal

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“…Recently, surface‐initiated atom transfer radical polymerization (ATRP) has been developed to control grafting density and grafting length independently on the matrix surface by adjusting initiator and monomer addition . More recently, surface‐initiated ATRP has also been used to prepare polymer‐grafted IEC resins pGMA‐M‐DMCH, which showed higher adsorption capacity for γ‐globulin than non‐grafted ion‐exchange resins . However, surface‐initiated ATRP requires a strict oxygen‐free environment to initiate the polymer grafting onto the matrix surface.…”

Section: Introductionmentioning

confidence: 99%

Poly(glycidyl methacrylate)‐grafted hydrophobic charge‐induction agarose resins with 5‐aminobenzimidazole as a functional ligand

Liu

Lin

Wang

et al. 2016

J of Separation Science

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Hydrophobic charge-induction chromatography is a new technology for antibody purification. To improve antibody adsorption capacity of hydrophobic charge-induction resins, new poly(glycidyl methacrylate)-grafted hydrophobic charge-induction resins with 5-aminobenzimidazole as a functional ligand were prepared. Adsorption isotherms, kinetics, and dynamic binding behaviors of the poly(glycidyl methacrylate)-grafted resins prepared were investigated using human immunoglobulin G as a model protein, and the effects of ligand density were discussed. At the moderate ligand density of 330 μmol/g, the saturated adsorption capacity and equilibrium constant reached the maximum of 140 mg/g and 25 mL/mg, respectively, which were both much higher than that of non-grafted resin with same ligand. In addition, effective pore diffusivity and dynamic binding capacity of human immunoglobulin G onto the poly(glycidyl methacrylate)-grafted resins also reached the maximum at the moderate ligand density of 330 μmol/g. Dynamic binding capacity at 10% breakthrough was as high as 76.3 mg/g when the linear velocity was 300 cm/h. The results indicated that the suitable polymer grafting combined with the control of ligand density would be a powerful tool to improve protein adsorption of resins, and new poly(glycidyl methacrylate)-grafted hydrophobic charge-induction resins have a promising potential for antibody purification applications.

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Ion-exchange separations of biomacromolecules on grafted and surface-modified polymers

2024

Ion-Exchange Chromatography and Related Techniques

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Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization

Cited by 17 publications

References 43 publications

Surface DEAE groups facilitate protein transport on polymer chains in DEAE‐modified‐and‐DEAE‐dextran‐grafted resins

Surface DEAE groups facilitate protein transport on polymer chains in DEAE‐modified‐and‐DEAE‐dextran‐grafted resins

Poly(glycidyl methacrylate)‐grafted hydrophobic charge‐induction agarose resins with 5‐aminobenzimidazole as a functional ligand

Ion-exchange separations of biomacromolecules on grafted and surface-modified polymers

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