Fabrication of fluorescent labeled ginseng polysaccharide nanoparticles for bioimaging and their immunomodulatory activity on macrophage cell lines

Akhter, Kazi Farida; Mumin, Abdul; Lui, Edmund M.K.; Charpentier, Paul A.

doi:10.1016/j.ijbiomac.2017.12.050

Cited by 36 publications

(26 citation statements)

References 43 publications

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“…Different types of chemical modifications have been performed to increase the versatility of alginate as biomaterials, taking advantage of its native OH or COOH functional groups, using alternative reaction routes, like oxidations (Liu et al, 2018 ), thiolations (Maleki et al, 2015 ), reductive-aminations (Rinaudo, 2011 ; Akhter et al, 2018 ), sulfations (Kerschenmeyer et al, 2017 ; Yu et al, 2017 ), esterifications (Ye et al, 2017 ), and amidations (Heo et al, 2017 ; Kondaveeti et al, 2018 ). Such approaches have been used either as intermediate steps to introduce specific reactive groups for further chemical modifications (Maleki et al, 2015 ; Liu et al, 2018 ; Pei et al, 2018 ), or to directly incorporate new moieties on alginate molecules.…”

Section: Biofunctionalization Of Alginate Hydrogels With Cell Instrucmentioning

confidence: 99%

“…Such approaches have been used either as intermediate steps to introduce specific reactive groups for further chemical modifications (Maleki et al, 2015 ; Liu et al, 2018 ; Pei et al, 2018 ), or to directly incorporate new moieties on alginate molecules. This allows to tune different features of alginate polymers and create derivatives with improved properties, such as bioactivity (Kerschenmeyer et al, 2017 ), mechanical properties (Liu et al, 2018 ), degradation rate (Fonseca et al, 2014a ), fluorescence (Akhter et al, 2018 ; Araujo et al, 2020 ), drug loading capacity or release profile (Ye et al, 2017 ), and antimicrobial activity (Kondaveeti et al, 2018 ), amongst others. For the design of artificial 3D microenvironments, the incorporation of moieties that specifically modulate cell-material interactions is pivotal.…”

Section: Biofunctionalization Of Alginate Hydrogels With Cell Instrucmentioning

confidence: 99%

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Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Neves

Moroni

Barrias

2020

Front. Bioeng. Biotechnol.

131

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The rational choice and design of biomaterials for biomedical applications is crucial for successful in vitro and in vivo strategies, ultimately dictating their performance and potential clinical applications. Alginate, a marine-derived polysaccharide obtained from seaweeds, is one of the most widely used polymers in the biomedical field, particularly to build three dimensional (3D) systems for in vitro culture and in vivo delivery of cells. Despite their biocompatibility, alginate hydrogels often require modifications to improve their biological activity, namely via inclusion of mammalian cell-interactive domains and fine-tuning of mechanical properties. These modifications enable the addition of new features for greater versatility and control over alginate-based systems, extending the plethora of applications and procedures where they can be used. Additionally, hybrid systems based on alginate combination with other components can also be explored to improve the mimicry of extracellular microenvironments and their dynamics. This review provides an overview on alginate properties and current clinical applications, along with different strategies that have been reported to improve alginate hydrogels performance as 3D matrices and 4D dynamic systems.

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Section: Biofunctionalization Of Alginate Hydrogels With Cell Instrucmentioning

confidence: 99%

Section: Biofunctionalization Of Alginate Hydrogels With Cell Instrucmentioning

confidence: 99%

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Neves

Moroni

Barrias

2020

Front. Bioeng. Biotechnol.

131

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“…Specifically, ginseng restores D-gal-induced memory deficits by enhancing hippocampal LPA1 receptor expression, LTP, and neurogenesis [12]. Ginseng polysaccharides and ginsenosides, which are the main active ingredients of the DST prescription, exert favorable curative effects on AD and other aging-related diseases [13][14][15]. However, the human body is a complicated system that exhibits dynamic metabolic changes and is affected by interactions between the intestinal flora and the host.…”

Section: Introductionmentioning

confidence: 99%

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Wang

Guo

et al. 2021

BioMed Research International

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Aging affects the brain function in elderly individuals, and Dushen Tang (DST) is widely used for the treatment of senile diseases. In this study, the protective effect of DST against memory impairment was evaluated through the Morris water maze (MWM) test and transmission electron microscopy (TEM). A joint analysis was also performed using LC-MS metabolomics and the microbiome. The MWM test showed that DST could significantly improve the spatial memory and learning abilities of rats with memory impairment, and the TEM analysis showed that DST could reduce neuronal damage in the hippocampus of rats with memory impairment. Ten potential biomarkers involving pyruvate metabolism, the synthesis and degradation of ketone bodies, and other metabolic pathways were identified by the metabolomic analysis, and it was found that 3-hydroxybutyric acid and lactic acid were involved in the activation of cAMP signaling pathways. The 16S rDNA sequencing results showed that DST could regulate the structure of the gut microbiota in rats with memory impairment, and these effects were manifested as changes in energy metabolism. These findings suggest that DST exerts a good therapeutic effect on rats with memory impairment and that this effect might be mainly achieved by improving energy metabolism. These findings might lead to the potential development of DST as a drug for the treatment of rats with memory impairment.

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“…Other alternatives include addition of biological and functional additives to the diet, such as moringa, mulberry, Chinese herbal medicine, tea polyphenol, dietary fiber, and ginseng polysaccharides. Among them, ginseng polysaccharides, due to their high content of polysaccharides, peptides, saponins, and other active substances, can be used as functional feed additives to promote food intake [8], improve immunity [9], and decrease the amount of abdominal fat and serum cholesterol [10] in broilers. Therefore, if ginseng polysaccharide is mixed with microbially fermented feed, it may have a positive impact on the development of new alternative antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken

et al. 2020

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Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine. Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development. Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides. The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum. In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected. Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens. A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla. At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups. Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio. At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance. Moreover, bacterial abundance was closely related to the jejunum histomorphology. Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio. Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt

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Fabrication of fluorescent labeled ginseng polysaccharide nanoparticles for bioimaging and their immunomodulatory activity on macrophage cell lines

Cited by 36 publications

References 43 publications

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken

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