Fabrication of Electrospun Scaffolds Incorporating an Amazonian Therapeutic Oil from the &lt;I&gt;Copaifera&lt;/I&gt; &lt;I&gt;Species&lt;/I&gt; for Wound Care Applications

Millás, Ana Luiza Garcia; McKean, Robert; Stevens, Robert S.; Yusuf, Mohammed; Silveira, João Vinícios Wirbitzki da; Puzzi, Maria Beatriz; Bittencourt, Edison

doi:10.1166/jbt.2014.1162

Cited by 11 publications

(10 citation statements)

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“…PLGA scaffolds were prepared as described previously (8). Briefly, PLGA (Purasorb, 5004; Purac Biomaterials, Gorinchem, The Netherlands) had a monomer ratio of 50:50 lactide to glycolide, a weight-averaged molecular weight of 40,00075,000.…”

Section: Methodsmentioning

confidence: 99%

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Platinum Blue Staining of Cells Grown in Electrospun Scaffolds

et al. 2014

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Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished from the scaffold fibers. Here we optimized the required concentration of platinum blue for imaging cells grown in scaffolds and show that a higher concentration causes platinum aggregation. Overall, platinum blue is a useful stain for imaging cells because of its enhanced contrast using scanning electron microscopy (SEM). In the future it would be useful to investigate cell growth and morphology using three-dimensional imaging methods.

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Section: Methodsmentioning

confidence: 99%

“…Contrast from the samples is achieved using heavy metals that scatter electrons well and adsorb to biological matter easily. Sputter coating the sample with heavy elements such as gold (8) is generally used. An alternative method is to stain the sample using heavy metal compounds such as lead citrate or phosphotungstic acid.…”

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Platinum Blue Staining of Cells Grown in Electrospun Scaffolds

et al. 2014

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“…Diâmetros de filamentos, espessura, análises de morfologia e porosidade dos scaffolds foram realizadas por microscopia eletrônica de varredura (MEV). Para análise dos ensaios in vitro foi utilizada microscopia eletrônica de varredura (MEV) segundo métodos propostos por Millás et al (2014) e Yusuf et al, (2014) (YUSUF et al, 2014;MILLÁS, 2016). As amostras dos ensaios in vivo foram coradas pelo método de hematoxilina-eosina (HE) e observadas em microscopia óptica (MO).…”

Section: Análise E Caracterização Do Materialsunclassified

Desenvolvimento De Arcabouços De Plga E Pldla Com Poros Interconectados Direcionados Para Ensaios De Cultura De Células Ósseas

Silva¹,

Melero²,

Domingues³

et al. 2019

A Aplicação Do Conhecimento Científico Nas Engenharias

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“…[1,2] Copaiba oil (CO) is a natural and biocompatible compound approved by the FDA, which has antibacterial, antimicrobial, antifungal, antiinflammatory, and analgesic properties. [3][4][5] Magnetic nanoparticles (MNPs) are nanostructured systems capable of performing therapeutic and diagnosis functions being capable of delivering drugs to precise targets and be used in hyperthermia treatments in the presence of an external magnetic field. [6,7] These characteristics of MNPs makes it a great potential alternative to decrease drug dosage administration.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of Magnetic Nanoparticles and Copaíba Oil in Poly(methyl methacrylate) Nanoparticles via Miniemulsion Polymerization for Biomedical Application

Cordeiro

Feuser

Araújo

et al. 2020

Macromolecular Symposia

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Polymeric nanoparticles (NPs) are used for drug delivery, mainly due to their ability to increase the selectivity and therapeutic efficacy of the encapsulated molecules. Alternative therapeutic compounds like magnetic nanoparticles (MNPs) and copaiba oil are alternatives for the development of more efficient and less aggressive therapeutic systems. The objective of the present work is to synthesize poly(methyl methacrylate) (PMMA) nanoparticles containing MNPs and copaiba oil (CO) via miniemulsion polymerization. The nanoparticles presented a mean diameter of ≈98 ± 1 nm with narrow particle size dispersion (0.1) and high colloidal stability with zeta potential below −50 ± −3 mV. The miniemulsion polymerization process allowed to incorporate the MNPs and the copaiba oil into the polymer matrix and the nanoparticles presented a saturation magnetization higher than ≈49 emu/g of MNPs and superparamagnetic properties. The evaluated PMMA‐MNPs‐CO concentrations, up to 200 µg mL−1 of MNPs, did not affect fibroblast cells viability.

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Fabrication of Electrospun Scaffolds Incorporating an Amazonian Therapeutic Oil from the <I>Copaifera</I> <I>Species</I> for Wound Care Applications

Cited by 11 publications

References 0 publications

Platinum Blue Staining of Cells Grown in Electrospun Scaffolds

Platinum Blue Staining of Cells Grown in Electrospun Scaffolds

Desenvolvimento De Arcabouços De Plga E Pldla Com Poros Interconectados Direcionados Para Ensaios De Cultura De Células Ósseas

Encapsulation of Magnetic Nanoparticles and Copaíba Oil in Poly(methyl methacrylate) Nanoparticles via Miniemulsion Polymerization for Biomedical Application

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