Fabrication of DNA Microarrays with Poly(<scp>l</scp>-glutamic acid) Monolayers on Gold Substrates for SPR Imaging Measurements

Chen, Yulin; Nguyen, Anh H.; Niu, Lifang; Corn, Robert M.

doi:10.1021/la804021t

Cited by 52 publications

(89 citation statements)

References 30 publications

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“…SPR imaging techniques have been used to study the sequence-specific adsorption of response regulator proteins to DNA [103,104], bioaffinity interactions in peptide arrays [105], proteins [106], and carbohydrate-protein interactions [107]. A very important feature in SPR imaging is the spatial resolution of the image that is determined by the propagation length of the SP at the interface between the metal and the analyte, which is dependent on the dielectric constant of the metal film, the refractive index of the analyte, and the frequency of the incident light (see Eq.…”

Section: Spr Imaging Techniques For Sensingmentioning

confidence: 99%

Sensitivity‐enhancement methods for surface plasmon sensors

Shalabney

Abdulhalim

2011

Laser & Photonics Reviews

443

254

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Surface plasmon resonance (SPR) sensors have been a mature technology for more than two decades now, however, recent investigations show continuous enhancement of their sensitivity and their lower detection limit. Together with the recent investigations in localized SPR phenomena, extraordinary optical transmission through nanoapertures in metals, and surface-enhanced spectroscopies, drastic developments are expected to revolutionize the field of optical biosensing. Sensitivity-enhancement (SE) techniques are reviewed focusing both on the physical transduction mechanisms and the system performance. In the majority of cases the SE is associated with the enhancement of the electromagnetic field overlap integral describing the interaction energy within the analyte. Other important mechanisms are the interaction between plasmons and excitons and between the analyte molecules and the metal surface. The lower detection limit can be reduced significantly if systems with high signal-to-noise ratio are used such as common-path interferometry, ellipsometry or polarimetry systems.

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Section: Spr Imaging Techniques For Sensingmentioning

confidence: 99%

Sensitivity‐enhancement methods for surface plasmon sensors

Shalabney

Abdulhalim

2011

Laser & Photonics Reviews

443

254

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“…To detect even lower concentrations of biomolecules with SPRI, a number of nanoparticle-based and surface enzyme-based signal amplification schemes have been implemented. 17-25 For example, we have previously shown that the method of RNase H-amplified SPRI can be used to detect ssDNA down to a concentration of 10 fM. 26,27 However, all of our previous studies have been performed with milliliter samples in a circulating flow cell format that creates a steady state concentration gradient from solution to the surface.…”

Section: Introductionmentioning

confidence: 99%

“…A combination of chemical attachment and surface enzymatic attachment reactions are used to create single-stranded DNA and RNA (ssDNA and ssRNA respectively) microarrays. 17,29 After array fabrication, the microchannels are flushed out with buffer solution, and then four separate 3 μL target volumes are introduced into the microchambers of the biochip. Real-time SPRI measurements are used to monitor the specific adsorption of ssDNA and two proteins, single stranded DNA binding protein and thrombin, onto the microarray elements from quiescent nanomolar solutions.…”

Section: Introductionmentioning

confidence: 99%

Rapid Microarray Detection of DNA and Proteins in Microliter Volumes with Surface Plasmon Resonance Imaging Measurements

2011

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A four chamber microfluidic biochip is fabricated for the rapid detection of multiple proteins and nucleic acids from microliter volume samples with the technique of surface plasmon resonance imaging (SPRI). The 18 mm × 18 mm biochip consists of four 3 μL microfluidic chambers attached to an SF10 glass substrate, each of which contains three individually addressable SPRI gold thin film microarray elements. The twelve element (4 × 3) SPRI microarray consists of gold thin film spots (1 mm2 area; 45 nm thickness) each in individually addressable 0.5 μL volume microchannels. Microarrays of single-stranded DNA and RNA (ssDNA and ssRNA respectively) are fabricated by either chemical and/or enzymatic attachment reactions in these microchannels; the SPRI microarrays are then used to detect femtomole amounts (nanomolar concentrations) of DNA and proteins (single stranded DNA binding protein and thrombin via aptamer-protein bioaffinity interactions). Microarrays of ssRNA microarray elements were also used for the ultrasensitive detection of zeptomole amounts (femtomolar concentrations) of DNA via the technique of RNase H-amplified SPRI. Enzymatic removal of ssRNA from the surface due to the hybridization adsorption of target ssDNA is detected as a reflectivity decrease in the SPR imaging measurements. The observed reflectivity loss was proportional to the log of the target ssDNA concentration with a detection limit of 10 fM or 30 zeptomoles (18,000 molecules). This enzymatic amplified ssDNA detection method is not limited by diffusion of ssDNA to the interface, and thus is extremely fast, requiring only 200 seconds in the microliter volume format.

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“…Maleimide-functionalized NTA was attached to the gold thin film microarray elements using a SATP strategy shown in Figure 4 that has been described and characterized previously 22 (Figure 4). The SPRI chips also include a thin hydrophobic Sigmacote film on the glass areas surrounding the gold microarray elements to prevent crosstalk during the fabrication process 19 . The NTA-functionalized chips were exposed to an aqueous 40 mM Cu II solution for 15 minutes and then rinsed with nanopure water.…”

Section: Resultsmentioning

confidence: 99%

A Microwell–Printing Fabrication Strategy for the On-Chip Templated Biosynthesis of Protein Microarrays for Surface Plasmon Resonance Imaging

2016

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A two-step templated, ribosomal biosynthesis/printing method for the fabrication of protein microarrays for surface plasmon resonance imaging (SPRI) measurements is demonstrated. In the first step, a sixteen component microarray of proteins is created in microwells by cell free on chip protein synthesis; each microwell contains both an in vitro transcription and translation (IVTT) solution and 350 femtomoles of a specific DNA template sequence that together are used to create approximately 40 picomoles of a specific hexahistidine-tagged protein. In the second step, the protein microwell array is used to contact print one or more protein microarrays onto nitrilotriacetic acid (NTA)-functionalized gold thin film SPRI chips for real-time SPRI surface bioaffinity adsorption measurements. Even though each microwell array element only contains approximately 40 picomoles of protein, the concentration is sufficiently high for the efficient bioaffinity adsorption and capture of the approximately 100 femtomoles of hexahistidine-tagged protein required to create each SPRI microarray element. As a first example, the protein biosynthesis process is verified with fluorescence imaging measurements of a microwell array containing His-tagged green fluorescent protein (GFP), yellow fluorescent protein (YFP) and mCherry (RFP), and then the fidelity of SPRI chips printed from this protein microwell array is ascertained by measuring the real-time adsorption of various antibodies specific to these three structurally related proteins. This greatly simplified two-step synthesis/printing fabrication methodology eliminates most of the handling, purification and processing steps normally required in the synthesis of multiple protein probes, and enables the rapid fabrication of SPRI protein microarrays from DNA templates for the study of protein-protein bioaffinity interactions.

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Fabrication of DNA Microarrays with Poly(l-glutamic acid) Monolayers on Gold Substrates for SPR Imaging Measurements

Cited by 52 publications

References 30 publications

Sensitivity‐enhancement methods for surface plasmon sensors

Sensitivity‐enhancement methods for surface plasmon sensors

Rapid Microarray Detection of DNA and Proteins in Microliter Volumes with Surface Plasmon Resonance Imaging Measurements

A Microwell–Printing Fabrication Strategy for the On-Chip Templated Biosynthesis of Protein Microarrays for Surface Plasmon Resonance Imaging

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