Fabrication of cylindrical bone graft substitute supported by reduced graphene-oxide and nanocurcumin to promotes the bone tissue development

Senthil, R.; Çakır, Sinem Yeygel; Kavukcu, Serdar Batıkan; Aruni, Wilson

doi:10.1016/j.matlet.2022.132475

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Moreover, Runx2 has been shown to affect the transcription of both the OCN and ALP genes. Runx2 also enhances the differentiation of multipotential mesenchymal ROB-C26 cells into mature osteoblasts via modulating OCN and ALP gene expression, as reported previously [20]. We therefore speculate that scaffolds may upregulate Runx2 levels at the site of bone injury, thereby causing OCN and ALP expression.…”

Section: Administration Of Polymer-gol-av Scaffolds Did Not Cause Hep...supporting

confidence: 81%

“…Some fractures feature bone loss; this is found in many pathological fractures resulting from bone disease (e.g., osteosarcoma), in fracture complications such as delayed union or nonunion, and in fractures due to gunshot wounds that lead to bone loss. Such fractures may require treatment using different forms of grafting and/or implantation treatment [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Hassan¹,

Mohammed²,

HAMEED³

2023

Nano Biomedicine and Engineering

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Osteoblasts have an essential role in the process of bone formation, and polymer-collagen-Aloe vera (AV) is known to stimulate osteoblast proliferation and maturation. In this translational study, the effects of scaffolds on bone healing and the potential mechanisms responsible were investigated using an animal model of bone defects. Here, following surgical introduction of a bone defect in the proximal femurs of male rabbits, the left femur was implanted with scaffolds for 21 days, then compared to the right femur, which served as a control. According to histological analyses, the use of scaffolds did not result in hepatotoxicity or nephrotoxicity. In contrast to the control group, imaging using X-ray transmission and microcomputed tomography revealed that scaffold implantation boosted the bone repair. In addition, microcomputed tomographic and bone histomorphometric assays in the scaffold-treated group exposed an expansion in the formation of new trabecular bone. Furthermore, scaffold implantation resulted in a considerable increase in trabecular bone thickness but a decrease in the trabecular parameter factor. Following scaffold implantation, the quantities of alkaline phosphatase and osteocalcin, biomarkers capable of simulating bone development, were found to have gradually increased. Overall, this translational study found that scaffolds can improve bone repair by increasing trabecular bone creation via upregulation of Runx2-mediated alkaline phosphatase and osteocalcin gene expression. Our findings therefore suggest that scaffolds can be used to treat bone problems such as deformities and fractures.

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Section: Administration Of Polymer-gol-av Scaffolds Did Not Cause Hep...supporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Hassan¹,

Mohammed²,

HAMEED³

2023

Nano Biomedicine and Engineering

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“…Bioceramic was prepared and used to design the implant dimensions according to the method used in our previous work. 10 In brief, DI were prepared by blending PVA glue, mineralized collagen, and Si-NPs with the help of using rotary mixer (MIXYVAC S, Italy), and the resultant mixture was loaded into the injection molding system (AV-YD Denture Injection system, Aixin Medical Equipment Co., Ltd. China) and pulled out with an appropriate glass rod. The produced DI was dried at 55°C at 70°C for 12 h. DI had a length of 2 to 4 cm, with an average diameter of 2–10 mm.…”

Section: Methodsmentioning

confidence: 99%

Mineralized Collagen Fiber-based Dental Implant: Novel Perspectives

Senthil,

Anitha,

Lakshmi

2023

Journal of Advanced Oral Research

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Aim: One of the problems that dental bone defects commonly face is less biocompatibility. Therefore, it is important to find effective natural dental materials to increase the rate of cell viability. In the present work, a blend of mineralized collagen fiber (MCF)/silica nanoparticles (Si-NPs) is used to develop a dental implant (DI), for their possible application in dental bone repair. Materials and Methods: This research study was to develop a technique for the fabrication of DI using natural materials. Accordingly, the present work provides DI, essentially by PVA (6g): MCF (1.5g): Si-NPs (0.8g): CaCO3 (1.0g) slurry into DI with other conventional implants. The DI was characterized by its mechanical, physicochemical, and biocompatibility study. The mechanical analysis was statistically different in all three time periods ( p < .05). Surface characterization of DI was carried out before and after immersion in the SBF. Results: The DI was excellent mechanical properties like compressive strength (24.22 ± 0.32 MPa) and elongation at break (16.51% ± 0.71%). The morphology of the DI showed a good pore size observed. Bioactivity test was observed on the Calcium/Phosphate of the DI. The biocompatibility of the study MTT (3-(4,5-dimethyl) thiazol-2-yl-2,5-dimethyl tetrazolium bromide) assay using the MG63 (human osteoblast cell line) has proven to more viable cell on the DI. Conclusion: The study has devised a process for using fish waste in the preparation of DI. The DI with the required strength, biocompatibility, and bone mineralization properties may be tried as a DI in large animals after obtaining the necessary approval.

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“…Graphene oxide (GO) with hydroxyl and carboxyl groups disperses well in solvents, and it opens up new possibilities for the manufacturing of GO-based hybrid composites. 11,12 Composite materials have a high surface/volume ratio, and strong adsorption capacity, and allow for less reaction of charge carriers at the point of contact involving the metal-containing oxides and the reduced graphene oxide. 13,14 Reduced graphene oxide (R-GO)-based nano biomaterials are an important topic of study in materials and have already been shown to have interesting applications in a wide range of medical fields.…”

Section: Introductionmentioning

confidence: 99%

Bone implant substitutes from synthetic polymer and reduced graphene oxide: Current perspective

Senthil

2023

Int J Artif Organs

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In the present work, bone implant materials (BIM) were produced, in sheet form which comprises epoxy resin (synthetic polymer) (ER), calcium carbonate (CaCO3), and reduced graphene oxide (R-GO), by open mold method, for the possibility uses in bone tissue engineering. The developed BIM was analyzed for its physico-chemical, mechanical, bioactivity test, antimicrobial study, and biocompatibility. The BIM had excellent mechanical properties such as tensile strength (194.44 + 0.21 MPa), flexural strength (278.76 + 0.41 MPa), and water absorption (02.61 + 0.24%). A pore size distribution study using the HR-SEM has proved the 180 and 255 μm average pore was observed in the BIM structure. The Bioactivity test of BIM was examined after being immersed in a simulated body fluids (SBF) solution. The result of BIM formed an excellent deposition of bone tube apatite crystals. High-resolution scanning electron microscopy (HR-SEM) morphology of the bone tube apatite crystals revealed the diameter size in the range from 100 ± 159 to 210 ± 188 nm. BIM has excellent antimicrobial characteristics against E. coli (8.75 + 0.06 mm) and S. aureus (9.82 + 0.08 mm). The biocompatibility of the study MTT (3-(4, 5-dimethyl) thiazol-2-yl-2, 5-dimethyl tetrazolium bromide) assay using the MG-63 (human osteoblast cell line) has proven to be the 78% viable cell presence in BIM. After receiving the necessary approval, the scaffold with the required strength and biocompatibility could be tested as a bone implant material in large animals.

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Fabrication of cylindrical bone graft substitute supported by reduced graphene-oxide and nanocurcumin to promotes the bone tissue development

Cited by 5 publications

References 9 publications

Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Mineralized Collagen Fiber-based Dental Implant: Novel Perspectives

Bone implant substitutes from synthetic polymer and reduced graphene oxide: Current perspective

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